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Registro Completo |
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
04/01/2018 |
Data da última atualização: |
30/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MALABARBA, J.; BUFFON, V.; MARIATH, J. E. A.; GAETA, M. L.; DORNELAS, M. C.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. |
Afiliação: |
Jaiana Malabarba, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Porto Alegre, RS, 91501-970, Brazil; VANESSA BUFFON, CNPUV; Jorge E. A. Mariath, Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Porto Alegre, RS, 91501- 970, Brazil; Marcos L. Gaeta, Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Porto Alegre, RS, 91501- 970, Brazil; Marcelo C. Dornelas, Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083-862, Brazil; Márcia Margis-Pinheiro, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Porto Alegre, RS, 91501-970, Brazil; Giancarlo Pasquali, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Porto Alegre, RS, 91501-970, Brazil; LUIS FERNANDO REVERS, CNPUV. |
Título: |
The MADS-box gene Agamous-like 11 is essential for seed morphogenesis in grapevine. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Journal of Experimental Botany, v. 68, n. 7, p. 1493-1506, mar. 2017. |
Idioma: |
Inglês |
Conteúdo: |
Despite the wide appreciation of seedless grapes, little is known about the molecular mechanisms that drive the stenospermocarpic seedless-type phenotype in grapevine. In order to address the molecular mechanisms that control seedlessness in grapevine, our study aimed to characterize VviAGL11, a class D MADS-box transcription factor gene that has been proposed as the major candidate gene involved in Vitis vinifera seed morphogenesis. VviAGL11 allelic variations in seeded and seedless grapevine cultivars were determined, and its correlations with allele-specific steady-state mRNA levels were investigated. VviAGL11 relative expression was significantly higher in seeds at 2, 4, and 6 weeks after fruit set, whereas in the seedless grape its transcript levels were extremely low in all stages analyzed. In situ hybridization revealed transcript accumulation specifically in the dual endotesta layer of the seeds, which is responsible for elongation and an increase of cell number, a necessary step to determine the lignification and the final seed size. No hybridization signals were visible in the seedless grapevine tissues, and a morphoanatomical analysis showed an apparent loss of identity of the endotesta layer of the seed traces. Ectopic expression of VviAGL11 in the Arabidopsis SEEDSTICK mutant background restored the wild-type phenotype and confirmed the direct role of VviAGL11 in seed morphogenesis, suggesting that depletion of its expression is responsible for the erroneous development of a highly essential seed layer, therefore culminating in the typical apirenic phenotype. Key words: Apireny, grapevine, in situ hybridization, seedlessness, Sultanine, VviAGL11. MenosDespite the wide appreciation of seedless grapes, little is known about the molecular mechanisms that drive the stenospermocarpic seedless-type phenotype in grapevine. In order to address the molecular mechanisms that control seedlessness in grapevine, our study aimed to characterize VviAGL11, a class D MADS-box transcription factor gene that has been proposed as the major candidate gene involved in Vitis vinifera seed morphogenesis. VviAGL11 allelic variations in seeded and seedless grapevine cultivars were determined, and its correlations with allele-specific steady-state mRNA levels were investigated. VviAGL11 relative expression was significantly higher in seeds at 2, 4, and 6 weeks after fruit set, whereas in the seedless grape its transcript levels were extremely low in all stages analyzed. In situ hybridization revealed transcript accumulation specifically in the dual endotesta layer of the seeds, which is responsible for elongation and an increase of cell number, a necessary step to determine the lignification and the final seed size. No hybridization signals were visible in the seedless grapevine tissues, and a morphoanatomical analysis showed an apparent loss of identity of the endotesta layer of the seed traces. Ectopic expression of VviAGL11 in the Arabidopsis SEEDSTICK mutant background restored the wild-type phenotype and confirmed the direct role of VviAGL11 in seed morphogenesis, suggesting that depletion of its expression is responsible for the erroneous dev... Mostrar Tudo |
Palavras-Chave: |
Apireny; Grapevine; Seed morphogenesis; Seedlessness; Sultanine; VviAGL11. |
Thesaurus Nal: |
Grapes; In situ hybridization. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170337/1/Malabarba-VviAGL11-1.pdf
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Marc: |
LEADER 02529naa a2200301 a 4500 001 2084258 005 2019-04-30 008 2017 bl uuuu u00u1 u #d 100 1 $aMALABARBA, J. 245 $aThe MADS-box gene Agamous-like 11 is essential for seed morphogenesis in grapevine.$h[electronic resource] 260 $c2017 520 $aDespite the wide appreciation of seedless grapes, little is known about the molecular mechanisms that drive the stenospermocarpic seedless-type phenotype in grapevine. In order to address the molecular mechanisms that control seedlessness in grapevine, our study aimed to characterize VviAGL11, a class D MADS-box transcription factor gene that has been proposed as the major candidate gene involved in Vitis vinifera seed morphogenesis. VviAGL11 allelic variations in seeded and seedless grapevine cultivars were determined, and its correlations with allele-specific steady-state mRNA levels were investigated. VviAGL11 relative expression was significantly higher in seeds at 2, 4, and 6 weeks after fruit set, whereas in the seedless grape its transcript levels were extremely low in all stages analyzed. In situ hybridization revealed transcript accumulation specifically in the dual endotesta layer of the seeds, which is responsible for elongation and an increase of cell number, a necessary step to determine the lignification and the final seed size. No hybridization signals were visible in the seedless grapevine tissues, and a morphoanatomical analysis showed an apparent loss of identity of the endotesta layer of the seed traces. Ectopic expression of VviAGL11 in the Arabidopsis SEEDSTICK mutant background restored the wild-type phenotype and confirmed the direct role of VviAGL11 in seed morphogenesis, suggesting that depletion of its expression is responsible for the erroneous development of a highly essential seed layer, therefore culminating in the typical apirenic phenotype. Key words: Apireny, grapevine, in situ hybridization, seedlessness, Sultanine, VviAGL11. 650 $aGrapes 650 $aIn situ hybridization 653 $aApireny 653 $aGrapevine 653 $aSeed morphogenesis 653 $aSeedlessness 653 $aSultanine 653 $aVviAGL11 700 1 $aBUFFON, V. 700 1 $aMARIATH, J. E. A. 700 1 $aGAETA, M. L. 700 1 $aDORNELAS, M. C. 700 1 $aMARGIS-PINHEIRO, M. 700 1 $aPASQUALI, G. 700 1 $aREVERS, L. F. 773 $tJournal of Experimental Botany$gv. 68, n. 7, p. 1493-1506, mar. 2017.
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Embrapa Uva e Vinho (CNPUV) |
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Registros recuperados : 5 | |
1. | | MALABARBA, J.; BUFFON, V.; WAIRICH, A.; GAETA, M. L.; MARIATH, J. E. A.; DORNELAS, M. C.; REVERS, L. F. Gene expression analysis of VVAG3 in grapevine reproductive tissues by in situ hybridization. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 3. Resumo.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Uva e Vinho. |
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2. | | CINTRA, L. A.; SOUZA, T. B. de; PARTEKA, L. M.; BARRETO, L. M.; PEREIRA, L. F. P.; GAETA, M. L.; GUYOT, R.; VANZELA, A. L. L. An 82 bp tandem repeat family typical of 3' non-coding end of Gypsy/TAT LTR retrotransposons is conserved in Coffea spp. pericentromeres. Genome, v.65, n. 3, p. 137-181, 2021. Date of Electronic Publication: 2021 Nov 02.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Café. |
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3. | | MALABARBA, J.; BUFFON, V.; MARIATH, J. E. A.; GAETA, M. L.; DORNELAS, M. C.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. The MADS-box gene Agamous-like 11 is essential for seed morphogenesis in grapevine. Journal of Experimental Botany, v. 68, n. 7, p. 1493-1506, mar. 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Uva e Vinho. |
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4. | | MALABARBA, J.; BUFFON, V.; WAIRICH, A.; GAETA, M. L.; MARIATH, J. E. A.; DORNELAS, M. C.; PASQUALI, G.; REVERS, L. F. Padrão de expressão espaço-temporal do gene VvAGL11 em tecidos reprodutivos de videira por hibridização in situ. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 11.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 7., Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2013. p. 18. Resumo.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Uva e Vinho. |
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5. | | MALABARBA, J.; BUFFON, V.; FRÂNCIO, L.; MARIATH, J. E. A.; GAETA, M. L.; DORNELAS, M. C.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. The transcription of agamous-like 11 is essential for seed morphogenesis in grapevine. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 27.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Uva e Vinho. |
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Registros recuperados : 5 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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