Data/hora: 24/06/2024 08:21:03

Biblioteca(s): 	Embrapa Pecuária Sudeste.	
Data corrente: 	16/02/2005	
Data da última atualização: 	11/05/2022	
Autoria: 	FUNES-HUACCA, M.; REGITANO, L. C. de A.; MURLLER, O.; CARILLHO, E.	
Afiliação: 	MARIBEL FUNES-HUACCA, USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; ODILO MUELLER, Agilent Technologies, Waldbronn, Germany; EMANUEL CARRILHO, USP.	
Título: 	Semiquantitative determination of Alicyclobacillus acidoterrestris in orange juice by reverse-transcriptase polymerase chain reaction and capillary electrophoresis: laser induced fluorescence using microchip technology	
Ano de publicação: 	2004	
Fonte/Imprenta: 	Electrophoresis, v.25, n.21-22, p.3860-3864, 2004.	
DOI: 	https://doi.org/10.1002/elps.200406105	
Idioma: 	Inglês	
Conteúdo: 	The semiquantitative detection ofAlicyclobacillus acidoterrrestrisin orange juice byreverse-transcriptase polymerase chain reaction (RT-PCR) with a linear dynamic rangeof 26105– 2 colony forming units (CFU)/mL in terms of cell count is described.Separation, detection, and quantification of the RT-PCR products were accomplishedusing the Agilent 2100 bioanalyzer in conjunction with the DNA 1000 LabChip kit. After0 and 12 h of enrichment, it was possible to generate a linear standard curve betweenthe amount of cells and amplicon concentration of RT-PCR and PCR products. Usingthis method, cell diminution was verified in samples of orange juice treated with a nat-ural inhibitor (Sapindus saponaria), determining the persistence of viable cells. Semi-quantitative RT-PCR using the Agilent 2100 bioanalyzer is a potentially useful approachfor rapidin vitrodetermination ofA. acidoterrestrisand monitoring of inhibitor sus-ceptibility for the orange juice-producing industry.	
Palavras-Chave: 	Miniaturization; Reversetranscriptase.	
Thesaurus Nal: 	Alicyclobacillus; Alicyclobacillus acidoterrestris; Microchip technology; Orange juice; Reverse transcriptase polymerase chain reaction.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Pecuária Sudeste (CPPSE)	
Exemplares: 	CPPSE - (15443-1) - UPC - AP - DD - PROCI-2004.00169 - HUA - 2004.00169 - DIS