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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
29/12/2015 |
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Data da última atualização: |
25/01/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUES, A. C. F.; MONERÓ, T. de O.; FRIGHETTO, R. T. S.; ALMEIDA, E. A. de. |
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Afiliação: |
ALINE CRISTINA FERREIRA RODRIGUES, IBILCE-UNESP; TATIANA DE OLIVEIRA MONERÓ, IBILCE-UNESP; ROSA TOYOKO SHIRAISHI FRIGHETTO, CNPMA; EDUARDO ALVES DE ALMEIDA, IBILCE-UNESP. |
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Título: |
E2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Environmental Science & Pollution Research, Berlin, v. 22, n. 22, p. 17367-17374, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
In the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia. MenosIn the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolate... Mostrar Tudo |
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Palavras-Chave: |
17b-Estradiol; Biotransformation enzymes; Hidrocarboneto aromático policíclico. |
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Thesagro: |
Oreochromis niloticus; Poluição da água; Substância tóxica; Tilápia nilótica. |
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Thesaurus Nal: |
Benzopyrenes; Polycyclic aromatic hydrocarbons; Water pollution. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02768naa a2200277 a 4500
001 2032529
005 2016-01-25
008 2015 bl uuuu u00u1 u #d
100 1 $aRODRIGUES, A. C. F.
245 $aE2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations.$h[electronic resource]
260 $c2015
520 $aIn the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17b-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia ( Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L) and E2 (5 μg L). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia.
650 $aBenzopyrenes
650 $aPolycyclic aromatic hydrocarbons
650 $aWater pollution
650 $aOreochromis niloticus
650 $aPoluição da água
650 $aSubstância tóxica
650 $aTilápia nilótica
653 $a17b-Estradiol
653 $aBiotransformation enzymes
653 $aHidrocarboneto aromático policíclico
700 1 $aMONERÓ, T. de O.
700 1 $aFRIGHETTO, R. T. S.
700 1 $aALMEIDA, E. A. de
773 $tEnvironmental Science & Pollution Research, Berlin$gv. 22, n. 22, p. 17367-17374, 2015.
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Embrapa Meio Ambiente (CNPMA) |
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| 44. |  | RODRIGUES, I. A.; FRIGHETTO, R. T. S.; RAMOS FILHO, L. O.; CAMPANHOLA, C.; RODRIGUES, G. S. Gestão participativa e desempenho ambiental de estabelecimentos de agroturismo na região de Itu(SP). In: PORTUGUEZ, A. P.; TAMANINI, E.; SANTIL, J. A. dos S.; CORRÊA, M. C. L.; FERRETTI, O., NIEHUES, V. D. Turismo no espaço rural: enfoque e perspectivas. São Paulo: Roca, 2006. p. 212-221.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 55. | | VALARINI, P. J.; FRIGHETTO, R. T. S.; SOUZA, M. D. de; SHIAVINATO, R. J. Desarrollo de nuevos indicadores para evaluación integrada de la capacidad productiva del suelo. CONGRESSO SEAE, 6.; CONGRESSO IBEROAMERICANO DE AGROECOLOGIA, 2.; ENCUENTRO DE ESTUDIANTES DE AGROECOLOGIA Y AGRICULTURA ECOLÓGICA, 1., 2004, Almería. Agroecologia referent e para la transición de los sistemas agrarios. Almeria: SEAE, 2004. p. 1267-1277.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 56. | | FRIGHETTO, R. T. S.; VALARINI, P. J.; RIZZO, LUIZ T. B.; CASTRO, S. S. distribuição espacial e temporal do teor de carbono em polissacarídeos, no horizonte de solos agrícolas: estudos de caso. In: ENCONTRO BRASILEIRO SOBRE SUBSTÂNCIAS HÚMICAS, 3., 1999, Santa Maria, RS. Anais...Matéria Orgânica e qualidade Ambiente:Universidade Federal de Santa Maria, Santa Maria, 1999. p.352-354| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 57. | | FERRACINI, V. L.; WATANABE, M. A.; FRIGHETTO, R. T. S.; SILOTO, R. C. Efeito repelente de extratos vegetais sobre a traca do tomateiro (Scrobipalpuloides absoluta, Lepidoptera, Gelechiidae). In: WORKSHOP SOBRE PRODUTOS NATURAIS NO CONTROLE DE PRAGAS, DOENCAS E PLANTAS DANINHAS, 1., 1990, Jaguariuna. Anais. Jaguariuna: EMBRAPA-CNPDA, 1990. p.49 (EMBRAPA-CNPDA. Documentos, 16).| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 203 | |
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