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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente; Embrapa Pecuária Sudeste. |
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Data corrente: |
10/10/2014 |
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Data da última atualização: |
29/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FIORENTINI, G.; CARVALHO, I. P. C.; MESSANA, J. D.; CASTAGNINO, P. S.; BERNDT, A.; CANESIN, R. C.; FRIGHETTO, R. T. S.; BERCHIELLI, T. T. |
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Afiliação: |
G. FIORENTINI, São Paulo State University; I. P. C. CARVALHO, São Paulo State University; J. D. MESSANA, São Paulo State University; P. S. CASTAGNINO, São Paulo State University; ALEXANDRE BERNDT, CPPSE; R. C. CANESIN, São Paulo State University; ROSA TOYOKO SHIRAISHI FRIGHETTO, CNPMA; T. T. BERCHIELLI, São Paulo State University. |
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Título: |
Effect of lipid sources with different fatty acid profiles on the intake, performance, and methane emissions of feedlot Nellore steers. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Animal Science, v. 92, n. 4, p. 1613-1620, apr. 2014. |
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DOI: |
10.2527/jas.2013-6868 |
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Idioma: |
Inglês |
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Conteúdo: |
The present study was conducted to evaluate the effect of lipids with different fatty acid profiles on the intake, performance, and enteric CH4 emission of Nellore steers. A total of 45 Nellore animals with an average initial BW of 419 ± 11 kg (at 15 ± 2 mo) were distributed in a completely randomized design consisting of 5 treatments and 9 replicates. The roughage feed was maize silage (600 g/kg on a DM basis) plus concentrate (400 g/kg on a DM basis). The dietary treatments were as follows: without additional fat (WF), palm oil (PO), linseed oil (LO), protected fat (PF; Lactoplus), and whole soybeans (WS). The lipid source significantly affected (P < 0.05) nutrient intake. The greatest intakes of DM, OM, and CP were observed in the animals that were fed the WF or PF diets, and the lowest intakes were observed in the animals that were fed the PO diet. Intake of NDF decreased (P < 0.05) with the addition of PO. Enteric methane emission (g/kg DMI) was reduced by an average of 30% when the animals were fed diets containing WS, LO, and PO (P < 0.05), and these diets caused a larger reduction in the energy loss in the form of methane compared to those without added fat and with added PF (3.3 vs. 4.7%). The different fatty acid profiles did not affect the backfat thickness or the loin eye area of the animals (P > 0.05). However, animals fed PO displayed lower daily weight gain (0.36 kg/d), feed efficiency (0.08 kg ADG/kg DM), HCW (245 kg), and hot yield percentage (52.6%) compared to animals that were fed the other diets. Therefore, PO compared to the other lipid sources used in this study reduces intake, performance, feed efficiency, and carcass yield. Therefore, PO is not suggested for feedlot-finished animals. MenosThe present study was conducted to evaluate the effect of lipids with different fatty acid profiles on the intake, performance, and enteric CH4 emission of Nellore steers. A total of 45 Nellore animals with an average initial BW of 419 ± 11 kg (at 15 ± 2 mo) were distributed in a completely randomized design consisting of 5 treatments and 9 replicates. The roughage feed was maize silage (600 g/kg on a DM basis) plus concentrate (400 g/kg on a DM basis). The dietary treatments were as follows: without additional fat (WF), palm oil (PO), linseed oil (LO), protected fat (PF; Lactoplus), and whole soybeans (WS). The lipid source significantly affected (P < 0.05) nutrient intake. The greatest intakes of DM, OM, and CP were observed in the animals that were fed the WF or PF diets, and the lowest intakes were observed in the animals that were fed the PO diet. Intake of NDF decreased (P < 0.05) with the addition of PO. Enteric methane emission (g/kg DMI) was reduced by an average of 30% when the animals were fed diets containing WS, LO, and PO (P < 0.05), and these diets caused a larger reduction in the energy loss in the form of methane compared to those without added fat and with added PF (3.3 vs. 4.7%). The different fatty acid profiles did not affect the backfat thickness or the loin eye area of the animals (P > 0.05). However, animals fed PO displayed lower daily weight gain (0.36 kg/d), feed efficiency (0.08 kg ADG/kg DM), HCW (245 kg), and hot yield percentage (52.6%) compare... Mostrar Tudo |
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Palavras-Chave: |
Emissão de metano; Fat whole soybeans; Intake; Oil palm; Oil protected. |
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Thesagro: |
Gado nelore; Linhaça; Metano; Novilho; Nutrição animal; Óleo vegetal. |
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Thesaurus Nal: |
Fatty acids; linseed; Linseed oil; Methane; Palm oils; Protected fat; Soybeans. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02962naa a2200433 a 4500
001 2003775
005 2023-03-29
008 2014 bl uuuu u00u1 u #d
024 7 $a10.2527/jas.2013-6868$2DOI
100 1 $aFIORENTINI, G.
245 $aEffect of lipid sources with different fatty acid profiles on the intake, performance, and methane emissions of feedlot Nellore steers.$h[electronic resource]
260 $c2014
520 $aThe present study was conducted to evaluate the effect of lipids with different fatty acid profiles on the intake, performance, and enteric CH4 emission of Nellore steers. A total of 45 Nellore animals with an average initial BW of 419 ± 11 kg (at 15 ± 2 mo) were distributed in a completely randomized design consisting of 5 treatments and 9 replicates. The roughage feed was maize silage (600 g/kg on a DM basis) plus concentrate (400 g/kg on a DM basis). The dietary treatments were as follows: without additional fat (WF), palm oil (PO), linseed oil (LO), protected fat (PF; Lactoplus), and whole soybeans (WS). The lipid source significantly affected (P < 0.05) nutrient intake. The greatest intakes of DM, OM, and CP were observed in the animals that were fed the WF or PF diets, and the lowest intakes were observed in the animals that were fed the PO diet. Intake of NDF decreased (P < 0.05) with the addition of PO. Enteric methane emission (g/kg DMI) was reduced by an average of 30% when the animals were fed diets containing WS, LO, and PO (P < 0.05), and these diets caused a larger reduction in the energy loss in the form of methane compared to those without added fat and with added PF (3.3 vs. 4.7%). The different fatty acid profiles did not affect the backfat thickness or the loin eye area of the animals (P > 0.05). However, animals fed PO displayed lower daily weight gain (0.36 kg/d), feed efficiency (0.08 kg ADG/kg DM), HCW (245 kg), and hot yield percentage (52.6%) compared to animals that were fed the other diets. Therefore, PO compared to the other lipid sources used in this study reduces intake, performance, feed efficiency, and carcass yield. Therefore, PO is not suggested for feedlot-finished animals.
650 $aFatty acids
650 $alinseed
650 $aLinseed oil
650 $aMethane
650 $aPalm oils
650 $aProtected fat
650 $aSoybeans
650 $aGado nelore
650 $aLinhaça
650 $aMetano
650 $aNovilho
650 $aNutrição animal
650 $aÓleo vegetal
653 $aEmissão de metano
653 $aFat whole soybeans
653 $aIntake
653 $aOil palm
653 $aOil protected
700 1 $aCARVALHO, I. P. C.
700 1 $aMESSANA, J. D.
700 1 $aCASTAGNINO, P. S.
700 1 $aBERNDT, A.
700 1 $aCANESIN, R. C.
700 1 $aFRIGHETTO, R. T. S.
700 1 $aBERCHIELLI, T. T.
773 $tJournal of Animal Science$gv. 92, n. 4, p. 1613-1620, apr. 2014.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
18/09/2015 |
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Data da última atualização: |
01/03/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, C. G.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; MARTINS, C. F.; BÁO, S. N. |
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Afiliação: |
HEIDI CHRISTINA BESSLER CUMPA, CPAC; ALVARO MORAES DA FONSECA NETO, CPAC; CARLOS FREDERICO MARTINS, CPAC; UNB. |
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Título: |
Effect of trichostatin a in cloned cattle embryo production by nuclear transfer with mesenchymal stem cells. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Animal Reproduction, v. 12, n. 3, p. 812, Jul./Sept. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Acetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and further for 16 or 21 h in medium cultivation. Subsequently, the embryos continued in culture in synthetic oviductal fluid (SOF) medium without TSA and the parthenogenetic control was performed in every manipulation. Five NT procedures were performed for each treatment (20 h, 25 h and without TSA). Fusion rates, cleavage and blastocyst production were compared by Tukey test (p <0.05). The cleavage rate of parthenogenetic embryos (93.45 ± 7.97) was higher than the cleavage rate of embryos without treatment (82.45 ± 5.59) but was statistically similar to embryos treated with 20 and 25 h (87.25 ± 8.41 and 85.54 ± 3.88, respectively). Still, there was no difference in cleavage rate between treated and untreated embryos. The blastocyst production rate on the seventh day of culture was superior to the parthenogenetic control (59.24 ± 11.75) compared to treatments for 20 h (36.22 ± 16.80), 25 h (33.66 ± 12.84) and without the use of TSA (32.70 ± 9.11), which did not differ. It can be concluded that the use of TSA had no significant effect in improving the rates of cleavage and development of bovine embryos by nuclear transfer with MSCs from adipose tissue. However, additional studies to evaluate the quality and embryos of the methylation pattern should be conducted to better understand the effects of TSA in embryos cloned with this cell type. MenosAcetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and... Mostrar Tudo |
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Palavras-Chave: |
Cloning; Epigenetic. |
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Thesagro: |
Clonagem. |
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Thesaurus NAL: |
histone deacetylase. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129960/1/34712.pdf
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Marc: |
LEADER 03483nam a2200205 a 4500
001 2024379
005 2016-03-01
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, C. G.
245 $aEffect of trichostatin a in cloned cattle embryo production by nuclear transfer with mesenchymal stem cells.
260 $aAnimal Reproduction, v. 12, n. 3, p. 812, Jul./Sept. 2015.$c2015
520 $aAcetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and further for 16 or 21 h in medium cultivation. Subsequently, the embryos continued in culture in synthetic oviductal fluid (SOF) medium without TSA and the parthenogenetic control was performed in every manipulation. Five NT procedures were performed for each treatment (20 h, 25 h and without TSA). Fusion rates, cleavage and blastocyst production were compared by Tukey test (p <0.05). The cleavage rate of parthenogenetic embryos (93.45 ± 7.97) was higher than the cleavage rate of embryos without treatment (82.45 ± 5.59) but was statistically similar to embryos treated with 20 and 25 h (87.25 ± 8.41 and 85.54 ± 3.88, respectively). Still, there was no difference in cleavage rate between treated and untreated embryos. The blastocyst production rate on the seventh day of culture was superior to the parthenogenetic control (59.24 ± 11.75) compared to treatments for 20 h (36.22 ± 16.80), 25 h (33.66 ± 12.84) and without the use of TSA (32.70 ± 9.11), which did not differ. It can be concluded that the use of TSA had no significant effect in improving the rates of cleavage and development of bovine embryos by nuclear transfer with MSCs from adipose tissue. However, additional studies to evaluate the quality and embryos of the methylation pattern should be conducted to better understand the effects of TSA in embryos cloned with this cell type.
650 $ahistone deacetylase
650 $aClonagem
653 $aCloning
653 $aEpigenetic
700 1 $aCUMPA, H. C. B.
700 1 $aFONSECA NETO, A. M. da
700 1 $aMARTINS, C. F.
700 1 $aBÁO, S. N.
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