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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
12/01/2011 |
Data da última atualização: |
19/01/2011 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA, M. F. e; MIROLI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; FREITAS ASTUA, J. de; MACHADO, M. A. |
Afiliação: |
Marina França e Silva, CNPDIA; Débora Marcondes Bastos Pereira Miroli, CNPDIA; Marcelo C. B. Cardinali, CNPDIA; Yvonne P. Mascarenhas, USP; Polyanna kelly Martins, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Marcos Antonio Machado, APTA. |
Título: |
Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
Idioma: |
Inglês |
Notas: |
120_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
Conteúdo: |
Several diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early citrus greening detection. MenosSeveral diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early ci... Mostrar Tudo |
Palavras-Chave: |
Candidatus Liberibater asiaticus; Plant disease. |
Thesagro: |
Bactéria; Doença de Planta; Fruta Cítrica; Greening. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02492nam a2200265 a 4500 001 1872725 005 2011-01-19 008 2010 bl uuuu u00u1 u #d 100 1 $aSILVA, M. F. e 245 $aApplication of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. 260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010 500 $a120_PS2 Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 520 $aSeveral diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early citrus greening detection. 650 $aBactéria 650 $aDoença de Planta 650 $aFruta Cítrica 650 $aGreening 653 $aCandidatus Liberibater asiaticus 653 $aPlant disease 700 1 $aMIROLI, D. M. B. P. 700 1 $aCARDINALI, M. C. B. 700 1 $aMASCARENHAS, Y. P. 700 1 $aMARTINS, P. K. 700 1 $aFREITAS ASTUA, J. de 700 1 $aMACHADO, M. A.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registros recuperados : 10 | |
1. | | BASTIANEL, M.; NOVELLI, V. M.; FREITAS-ASTUA, J. de. Field assessments reveal different levels of susceptibility among mandarins and hybrids to Citrus Leprosis Virus c. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 129.PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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2. | | RODRIGUES, A. S.; BARBOSA, C. de J.; SOARES FILHO, W. dos S.; FREITAS-ASTUA, J. de. Citrus rootstock hybrids tolerants to citrus tristeza virus and Bahia bark scaling disease. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 106_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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3. | | KUBO, K. S.; ARENA, G. D.; KITAJIMA, E. W; MACHADO, M. A.; FREITAS-ASTUA, J. de. Transmission of citrus leprosis virus c (CiLV-C) to Arabidopsis thaliana by Brevipalpus phoenicis mites. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 085 PS1
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
4. | | CUNHA, B. A.; NUNES, M. A.; FREITAS-ASTUA, J. de; BERGAMINI, M. P.; BASTIANEL, M.; NOVELLI, V. M. Different symptomatic tissues as sources of inoculum to citrus leprosis virus c (CILV-C). In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 128-PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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7. | | SILVA, M. F. e; MIROLI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; FREITAS ASTUA, J. de; MACHADO, M. A. Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 120_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
8. | | FREITAS ASTUA, J. de; KUBO, K. S.; KITAJIMA, E. W.; BASTIANEL, M.; COSTA, F. M.; NOVELLI, V. M.; MACHADO, M. A. The leprosis pathosystem: interactions between host, virus and vector. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS,18., CAMPINAS, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 009 PSO
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
9. | | LOCALI-FABRIS, E. C.; MAFRA, V. S.; MARTINS, P. K.; RIBEIRO- ALVES, M.; FRANCISCO, C. S.; FREITAS ASTUA, J. de; KISHI, L. T.; MACHADO, M. A. Gene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 0058 PSO
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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10. | | CÁCERES, S.; AGUIRRE, A.; COSTA, N.; SEGAÑA, L. G.; FARIÑA, N.; FREITAS ASTUA, J. de; MORAES, G. J.; TASSI, A. D.; KITAJIMA, E. W. Present situation of the citrus leprosis in Argentina and Paraguay. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 054 PSO.
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 10 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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