Data/hora: 18/06/2024 04:52:36

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 09/11/2017
Data da última atualização: | 23/09/2019
Autoria: | MONTEIRO, J. P.; SANTOS, J. M. L. dos; ANDRÉ, W. P. P.; VASCONCELOS, J. F.; FROTA, G. A.; RIBEIRO, W. L. C.; FREITAS, E. P. de; MACEDO, I. T. F.; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L.
Afiliação: | JOMAR PATRICIO MONTEIRO, CNPC; Jessica Maria Leite dos Santos, Pós-graduação - Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; Weibson Paz Pinheiro Andre, UECe - Fortaleza, CE, Brazil; Janaelia Ferreira Vasconcelos, Universidade Estadual do Vale do Acaraú (UVA) - Sobral, CE, Brazil; Gracielle Araújo Frota, UVA - Sobral, CE, Brazil; Wesley Lyeverton Correia Ribeiro, UECe - Fortaleza, CE, Brazil; Edilson Pereira de Freitas; Iara Tersia Freitas Macedo; MARCEL TEIXEIRA, CNPC; LUIZ DA SILVA VIEIRA, CNPC; Claudia Maria Leal Bevilaqua, UECe - Fortaleza, CE, Brazil.
Título: | Molecular diagnostic for levamisol resistance in Haemonchus contortus.
Ano de publicação: | 2017
Fonte/Imprenta: | In: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017. p. 381. Abstract 4592.
Idioma: | Inglês
Notas: | In conjunction with 53rd MSPTM Annual Conference.
Conteúdo: | Abstract: In tropical areas, Haemonchus contortus is the leading cause of production losses in small ruminant herds and its control is traditionally done through the utilization of synthetic anthelmintics. Levamisole, an imidazothiazole derivative, is widely used in Brazil and the occurrence of resistance is not uncommon. The genetic base for levamisole resistance in H. contortus is still under investigation, but it has been recently associated with a 63 bp deletion in the Hco-acr-8 gene, which codes for one of the subunits of a levamisole-sensistive acetylcholine receptor. Here we describe a real time PCR test for the detection and quantification of the presence and absence of this deletion. Reactions contained 12.5ul 2 × Fast Start Universal SYBR Green Master Mix (Roche, West Sussex, UK), 0.3 pmol/ul of each primer (forward and reverse), 50 ng of DNA and water for a total volume of 25?l. Amplification conditions for were: 95 oC for 10 min and 35 cycles at 95 oC for 15 s and at 56 ºC for 30 s. We tested the assay in two known H. contortus isolates, one resistant (Kokstad isolate - KOK) and another susceptible (Inbred-susceptible-Edinburgh - ISE). We also used the test to characterize a local H. contortus population previously exposed to levamisole. Preliminay results are in agreement with previously reported data as only the resistant allele was detected in the KOK isolate and both alleles were detected in the ISE isolate suggesting that this test may be useful in the fast detection of levamisole resistance in H. contortus.
Palavras-Chave: | Resistance to anthelmintics.
Thesagro: | Anti-Helmíntico; Caprino; Haemonchus Contortus; Ovino; Resistência a produtos químicos.
Thesaurus Nal: | Anthelmintics; Brazil; Drug resistance; Goats; Levamisole; Sheep; Small ruminants.
Categoria do assunto: | H Saúde e Patologia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/166470/1/cnpc-2017-Molecular-diagnostic.pdf
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (35621-1) - UPC - RA - DD -  -  -  - DIS