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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
09/08/1999 |
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Data da última atualização: |
20/06/2018 |
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Autoria: |
FREITAS, A. L. F. |
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Título: |
Substratos endogenos na determinacao da atividade proteolitica em cotiledones de Vigna unguiculata (L.) Walp. cv. serido. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
Fortaleza: UFC, 1982. |
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Páginas: |
55p. |
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Idioma: |
Português |
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Notas: |
Tese Mestrado. |
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Conteúdo: |
A atividade proteolitica em cotiledones de Vigna unguiculata (L.) Walp. cv. serido foi determinada usando-se como substrato proteinas diazotizadas de sementes quiescentes e como fonte de enzima extrato de cotiledones de sementes quiescentes e germinantes. Os resultados sugerem que o sistema enzimatico responsavel pela hidrolise das azoalbuminas e azoglobulinas e diferente daqueles responsaveis pela hidrolise de caseina, hemoglobina, a-N-Benzoil-DL-arginina-p-nitroanilida e L-Leucina-p-nitroanilida. |
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Palavras-Chave: |
Caupi; Feijao macassar. |
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Thesagro: |
Atividade Enzimática; Vigna Unguiculata. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01029nam a2200181 a 4500
001 1056046
005 2018-06-20
008 1982 bl uuuu m 00u1 u #d
100 1 $aFREITAS, A. L. F.
245 $aSubstratos endogenos na determinacao da atividade proteolitica em cotiledones de Vigna unguiculata (L.) Walp. cv. serido.
260 $aFortaleza: UFC$c1982
300 $a55p.
500 $aTese Mestrado.
520 $aA atividade proteolitica em cotiledones de Vigna unguiculata (L.) Walp. cv. serido foi determinada usando-se como substrato proteinas diazotizadas de sementes quiescentes e como fonte de enzima extrato de cotiledones de sementes quiescentes e germinantes. Os resultados sugerem que o sistema enzimatico responsavel pela hidrolise das azoalbuminas e azoglobulinas e diferente daqueles responsaveis pela hidrolise de caseina, hemoglobina, a-N-Benzoil-DL-arginina-p-nitroanilida e L-Leucina-p-nitroanilida.
650 $aAtividade Enzimática
650 $aVigna Unguiculata
653 $aCaupi
653 $aFeijao macassar
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Embrapa Meio-Norte (CPAMN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
07/11/2005 |
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Data da última atualização: |
07/11/2005 |
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Autoria: |
CHEN, W. -M.; FARIA, S. M. de; STRALIOTTO, R.; PITARD, R. M.; SIMÕES-ARAÚJO, J. L.; CHOU, J. -H.; CHOU, Y. -J.; BARRIOS, E.; PRESCOTT, A. R.; ELLIOTT, G. N.; SPRENT, J. I.; YOUNG, J. P. W.; JAMES, E. K. |
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Título: |
Proof that Burkholderia strains form effective symbioses with legumes> a study of novel Mimosa-nodulating strains from south America. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 71, n. 11, p. 7461-7471, nov. 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other ß-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known ß-rhizobial, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N2-fixing nodules on their original host, Mimosa bimucronata (Br3461) and Mimosa priga (MAP3-5), and hence this confirms strongly that Burkholderia strains can from effective symbioses with legumes. MenosTwenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other ß-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known ß-rhizobial, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N2-fixing nodules on their original host, Mimosa bimucronata (... Mostrar Tudo |
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Palavras-Chave: |
Biological nitrogen fixation; BNF; Burkholderia caribensis; Burkholderia phymatum; Burkholderia tuberum; FBN; Fixação biológica de mitrogênio; Mimosa bumucronata. |
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Thesagro: |
Ácido Nucléico; DNA; Simbiose. |
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Thesaurus NAL: |
Mimosa pigra; Mimosa pudica; nucleic acids; symbiosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02937naa a2200445 a 4500
001 1597242
005 2005-11-07
008 2005 bl --- 0-- u #d
100 1 $aCHEN, W. -M.
245 $aProof that Burkholderia strains form effective symbioses with legumes> a study of novel Mimosa-nodulating strains from south America.
260 $c2005
520 $aTwenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other ß-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known ß-rhizobial, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N2-fixing nodules on their original host, Mimosa bimucronata (Br3461) and Mimosa priga (MAP3-5), and hence this confirms strongly that Burkholderia strains can from effective symbioses with legumes.
650 $aMimosa pigra
650 $aMimosa pudica
650 $anucleic acids
650 $asymbiosis
650 $aÁcido Nucléico
650 $aDNA
650 $aSimbiose
653 $aBiological nitrogen fixation
653 $aBNF
653 $aBurkholderia caribensis
653 $aBurkholderia phymatum
653 $aBurkholderia tuberum
653 $aFBN
653 $aFixação biológica de mitrogênio
653 $aMimosa bumucronata
700 1 $aFARIA, S. M. de
700 1 $aSTRALIOTTO, R.
700 1 $aPITARD, R. M.
700 1 $aSIMÕES-ARAÚJO, J. L.
700 1 $aCHOU, J. -H.
700 1 $aCHOU, Y. -J.
700 1 $aBARRIOS, E.
700 1 $aPRESCOTT, A. R.
700 1 $aELLIOTT, G. N.
700 1 $aSPRENT, J. I.
700 1 $aYOUNG, J. P. W.
700 1 $aJAMES, E. K.
773 $tApplied and Environmental Microbiology, Washington$gv. 71, n. 11, p. 7461-7471, nov. 2005.
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