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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
03/02/2022 |
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Data da última atualização: |
12/09/2022 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
BOARI, A. de J.; CORDOVIL, G. A.; KAUFFMANN, C. M.; GAVINHO, B. E. S.; QUADROS, A. F. F.; PANTOJA, K. F. C.; GOMES JUNIOR, R. A.; FREIRE FILHO, F. R.; OLIVEIRA, R. P. de; KITAJIMA, E. W. |
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Afiliação: |
ALESSANDRA DE JESUS BOARI, CPATU; GABRIELA A. CORDOVIL, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; CATERYNNE M. KAUFFMANN, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; BRENDA E. S. GAVINHO, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; AYANE F. F. QUADROS, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; KÉSSIA F. C. PANTOJA, UNIVERSIDADE ESTADUAL PAULISTA; RUI ALBERTO GOMES JUNIOR, CPATU; FRANCISCO RODRIGUES FREIRE FILHO, CPATU; RAIMUNDO PARENTE DE OLIVEIRA, CPATU; ELLIOT W. KITAJIMA, ESCOLA SUPERIOR DE AGRICULTURA "LUIZ DE QUEIROZ". |
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Título: |
Bean common mosaic virus infecting manteiguinha cowpea (Vigna unguiculata): seed transmission, evaluation of yield loss, and genetic resistance. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 47, n. 3, p. 450-455, June 2022. |
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DOI: |
https://doi.org/10.1007/s40858-021-00490-w |
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Idioma: |
Inglês |
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Conteúdo: |
The manteiguinha type cowpea has extra-small grains with a creamy coloration and constitutes an important component for the culinary in the state of Pará, Brazil, with potential for acceptance in the whole country. A high incidence of plants presenting symptoms of viral infection, such as mosaic, leaf rolling, leaf epinasty, and vein necrosis, was observed in a genetic improvement assay of this cowpea cultivar in the experimental field of Embrapa/Amazônia Oriental in Belém, PA. After negative results for detection of Cucumber mosaic virus, Cowpea aphid-borne mosaic virus, and Cowpea severe mosaic virus by ELISA, the potential agent of the disease was identified as bean common mosaic virus (BCMV). For that, an RT-PCR assay was conducted using primers MJ1 and MJ2, universal for potyviruses, followed by sequencing of the amplified fragment, part of the coat protein gene. The sequences of two isolates (Br:Para:1 ? access MK431152 and Br:Para:2 ? access MK431153) showed 99% nucleotide and 100% aa identity with BCMV isolates from Taiwan, Thailand, the USA, and China. Cytopathology revealed the presence of Edwardson?s type I cylindrical inclusions, typical of potyvirus infection, in the cytoplasm of symptomatic leaf cells. Assays performed to estimate seed transmission of BCMV-Br:Para:1 isolate by infected manteiguinha cowpea indicated a transmission rate ca. 35%. Yield loss evaluation assays of genotype TUC 170 of manteiguinha cowpea indicated a 53% reduction in grain production and 47% fewer pods/plant. Genotype TUC 178, registered as cultivar BRS Natalina, revealed to be immune and may serve in BCMV resistance breeding programs MenosThe manteiguinha type cowpea has extra-small grains with a creamy coloration and constitutes an important component for the culinary in the state of Pará, Brazil, with potential for acceptance in the whole country. A high incidence of plants presenting symptoms of viral infection, such as mosaic, leaf rolling, leaf epinasty, and vein necrosis, was observed in a genetic improvement assay of this cowpea cultivar in the experimental field of Embrapa/Amazônia Oriental in Belém, PA. After negative results for detection of Cucumber mosaic virus, Cowpea aphid-borne mosaic virus, and Cowpea severe mosaic virus by ELISA, the potential agent of the disease was identified as bean common mosaic virus (BCMV). For that, an RT-PCR assay was conducted using primers MJ1 and MJ2, universal for potyviruses, followed by sequencing of the amplified fragment, part of the coat protein gene. The sequences of two isolates (Br:Para:1 ? access MK431152 and Br:Para:2 ? access MK431153) showed 99% nucleotide and 100% aa identity with BCMV isolates from Taiwan, Thailand, the USA, and China. Cytopathology revealed the presence of Edwardson?s type I cylindrical inclusions, typical of potyvirus infection, in the cytoplasm of symptomatic leaf cells. Assays performed to estimate seed transmission of BCMV-Br:Para:1 isolate by infected manteiguinha cowpea indicated a transmission rate ca. 35%. Yield loss evaluation assays of genotype TUC 170 of manteiguinha cowpea indicated a 53% reduction in grain production a... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Feijão; Vigna Unguiculata. |
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Thesaurus Nal: |
Potyvirus. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02596naa a2200289 a 4500
001 2139611
005 2022-09-12
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-021-00490-w$2DOI
100 1 $aBOARI, A. de J.
245 $aBean common mosaic virus infecting manteiguinha cowpea (Vigna unguiculata)$bseed transmission, evaluation of yield loss, and genetic resistance.$h[electronic resource]
260 $c2022
520 $aThe manteiguinha type cowpea has extra-small grains with a creamy coloration and constitutes an important component for the culinary in the state of Pará, Brazil, with potential for acceptance in the whole country. A high incidence of plants presenting symptoms of viral infection, such as mosaic, leaf rolling, leaf epinasty, and vein necrosis, was observed in a genetic improvement assay of this cowpea cultivar in the experimental field of Embrapa/Amazônia Oriental in Belém, PA. After negative results for detection of Cucumber mosaic virus, Cowpea aphid-borne mosaic virus, and Cowpea severe mosaic virus by ELISA, the potential agent of the disease was identified as bean common mosaic virus (BCMV). For that, an RT-PCR assay was conducted using primers MJ1 and MJ2, universal for potyviruses, followed by sequencing of the amplified fragment, part of the coat protein gene. The sequences of two isolates (Br:Para:1 ? access MK431152 and Br:Para:2 ? access MK431153) showed 99% nucleotide and 100% aa identity with BCMV isolates from Taiwan, Thailand, the USA, and China. Cytopathology revealed the presence of Edwardson?s type I cylindrical inclusions, typical of potyvirus infection, in the cytoplasm of symptomatic leaf cells. Assays performed to estimate seed transmission of BCMV-Br:Para:1 isolate by infected manteiguinha cowpea indicated a transmission rate ca. 35%. Yield loss evaluation assays of genotype TUC 170 of manteiguinha cowpea indicated a 53% reduction in grain production and 47% fewer pods/plant. Genotype TUC 178, registered as cultivar BRS Natalina, revealed to be immune and may serve in BCMV resistance breeding programs
650 $aPotyvirus
650 $aDoença de Planta
650 $aFeijão
650 $aVigna Unguiculata
700 1 $aCORDOVIL, G. A.
700 1 $aKAUFFMANN, C. M.
700 1 $aGAVINHO, B. E. S.
700 1 $aQUADROS, A. F. F.
700 1 $aPANTOJA, K. F. C.
700 1 $aGOMES JUNIOR, R. A.
700 1 $aFREIRE FILHO, F. R.
700 1 $aOLIVEIRA, R. P. de
700 1 $aKITAJIMA, E. W.
773 $tTropical Plant Pathology$gv. 47, n. 3, p. 450-455, June 2022.
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| 9. |  | FREIRE FILHO, F. R. Melhoramento de feijão-caupi no Brasil. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. p. 235.| Tipo: Resumo em Anais de Congresso |
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