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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
07/04/2004 |
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Autoria: |
SANCHEZ, O; RESTREPO, S; DUQUE, M. C; FREGENE, M; BONIERBALE, M; VERDIER, V. |
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Título: |
Application of AFLP for genetic characterization of cassava. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v.17, p.43, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cassava Bacterial Blight (CB-B), major disease in Latin America and Africa, is caused by Xanthomonas axonopodis pv. Manihot (Xam). Resistance is thought to be polygenic and additively inherited. 93 -varieties of Manihot esculenta (Crantz) from the cassava core collection CIAT went analyzed by AFLPs (Amplified Fragment Length polymorphism), and for ressistance to cassava bacterial blight. AFLP analysis was performed on all plant material using two primer combinations. A level of 79,2% polymorphism was found. The phenogram obtained shows betwe 74% and 96% of genetic similarity among all cassava accessions analyzed, The analysis permitted unique identification of each individual indicating that the sample used does not contain genetic duplicates. Variation in the reaction to Xam strain (strains CIAT 1177 and OrstX27) was observed among the 93 varieties tested for resistance to CBB. The correlation coefficient between resistance to Xam strains under controlled conditions was 0.53 suggesting the independence of resistance to each strain. Multiple correspondence analysis showed a random distribution of the resistance/susceptible response, with respect to overall genetic diversity measured by AFLP. A total heterozygosity index was calculated to find the diversity within clusters as among them. Our results demonstrate that AFLP anaysis is an effective means of providing quantitative estimates of genetic similarities among cassava accessions. We also demonstrate that resistance to CCB is broadly distributed in cassava germplasm. MenosAbstract: Cassava Bacterial Blight (CB-B), major disease in Latin America and Africa, is caused by Xanthomonas axonopodis pv. Manihot (Xam). Resistance is thought to be polygenic and additively inherited. 93 -varieties of Manihot esculenta (Crantz) from the cassava core collection CIAT went analyzed by AFLPs (Amplified Fragment Length polymorphism), and for ressistance to cassava bacterial blight. AFLP analysis was performed on all plant material using two primer combinations. A level of 79,2% polymorphism was found. The phenogram obtained shows betwe 74% and 96% of genetic similarity among all cassava accessions analyzed, The analysis permitted unique identification of each individual indicating that the sample used does not contain genetic duplicates. Variation in the reaction to Xam strain (strains CIAT 1177 and OrstX27) was observed among the 93 varieties tested for resistance to CBB. The correlation coefficient between resistance to Xam strains under controlled conditions was 0.53 suggesting the independence of resistance to each strain. Multiple correspondence analysis showed a random distribution of the resistance/susceptible response, with respect to overall genetic diversity measured by AFLP. A total heterozygosity index was calculated to find the diversity within clusters as among them. Our results demonstrate that AFLP anaysis is an effective means of providing quantitative estimates of genetic similarities among cassava accessions. We also demonstrate that resi... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02062naa a2200181 a 4500
001 1651816
005 2004-04-07
008 1998 bl uuuu u00u1 u #d
100 1 $aSANCHEZ, O
245 $aApplication of AFLP for genetic characterization of cassava.
260 $c1998
520 $aAbstract: Cassava Bacterial Blight (CB-B), major disease in Latin America and Africa, is caused by Xanthomonas axonopodis pv. Manihot (Xam). Resistance is thought to be polygenic and additively inherited. 93 -varieties of Manihot esculenta (Crantz) from the cassava core collection CIAT went analyzed by AFLPs (Amplified Fragment Length polymorphism), and for ressistance to cassava bacterial blight. AFLP analysis was performed on all plant material using two primer combinations. A level of 79,2% polymorphism was found. The phenogram obtained shows betwe 74% and 96% of genetic similarity among all cassava accessions analyzed, The analysis permitted unique identification of each individual indicating that the sample used does not contain genetic duplicates. Variation in the reaction to Xam strain (strains CIAT 1177 and OrstX27) was observed among the 93 varieties tested for resistance to CBB. The correlation coefficient between resistance to Xam strains under controlled conditions was 0.53 suggesting the independence of resistance to each strain. Multiple correspondence analysis showed a random distribution of the resistance/susceptible response, with respect to overall genetic diversity measured by AFLP. A total heterozygosity index was calculated to find the diversity within clusters as among them. Our results demonstrate that AFLP anaysis is an effective means of providing quantitative estimates of genetic similarities among cassava accessions. We also demonstrate that resistance to CCB is broadly distributed in cassava germplasm.
700 1 $aRESTREPO, S
700 1 $aDUQUE, M. C
700 1 $aFREGENE, M
700 1 $aBONIERBALE, M
700 1 $aVERDIER, V.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv.17, p.43, nov., 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 19 | |
| 4. |  | FARIAS, A. R. N.; BELLOTTI, A. C.; FREGENE, M.; SILVA, A. F.; ALVES, A. A. C. Avaliação de híbridos interespecíficos de mandioca para resistência a artrópodes-praga em condições semi-áridas. In: CONGRESSO BRASILEIRO DA MANDIOCA, 12., 2007, Paranavaí. Mandioca: bioenergia, alimento e renda. Botucatu: CERAT- UNESP, [2007].| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|    |
| 5. | | BELLOTTI, A.; FREGENE, M.; ALVES, A.; OKOGBENIN, E.; EGESI, C.; OKAI, E.; BAGUMA, Y.; PARIYO, A. Development of low-cost technologies for pyramiding useful genes from wild relatives of cassava into elite progenitors. In: CGIAR GENERATION CHALLENGE PROGRAMME ANNUAL RESEARCH MEETING, 2008, Bangkok, Thailand. Project abstracts... Mexico, DF: Generation Challenge Programme, 2008. p. 36-37.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | ALVES, A.; FREGENE, M.; SETTER, T.; DUQUE, L.; FERGUSON, M.; MKAMILO, G. Identifying the physiological and genetic traits related to drought tolerance mechanisms in cassava. In: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent. Cassava: meeting the challenges of the new millennium. Ghent:: IPBO, 2008. p. 142. S10-6.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | ALVES, A. A. C.; MENDES, R. A.; FREGENE, M.; BELLOTTI, A. Experiências em pré-melhoramento de mandioca : utilização do potencial de espécies silvestres de mandioca como fonte de resistência a estresses bióticos e abióticos. In: LOPES, M. A.; FÁVERO, A. P. FERREIRA, M. A. J. da; FALEIRO, F. G. (Org.). Curso internacional de pré-melhoramento de plantas. Brasília, DF : Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 143-146. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 185).| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | ALVES, A.; SETTER, T.; FREGENE, M.; FERGUSON, M.; DUQUE, L.; MUTEGI, R.; MKAMILO, G. Traits related to drought tolerance mechanisms in cassava. In: CGIAR GENERATION CHALLENGE PROGRAMME ANNUAL RESEARCH MEETING, 2008, Bangkok, Thailand. Poster abstracts... Mexico, DF: Generation Challenge Programme, 2008. p. 53.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | DITA, M. A.; JESUS, J. de; SILVA, A. F.; BELLOTTI, A.; FREGENE, M.; ALVES, A. A. C. Avaliação de híbridos interespecíficos de mandioca para a resistência a doenças. In: CONGRESSO BRASILEIRO DA MANDIOCA, 12., 2007, Paranavaí. Mandioca: bioenergia, alimento e renda. Botucatu: CERAT- UNESP, [2007].| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 12. | | MUTEGI, R.; FERGUSON, M.; MAASS, B. L.; MKAMILO, G.; KAMAU, J.; ADJEBENG-DANQUAH, J.; ALVES, A.; SETTER, T.; FREGENE, M. Field evaluation of cassava varieties under drought stress in Kenia, Tanzania and Ghana. In: CGIAR GENERATION CHALLENGE PROGRAMME ANNUAL RESEARCH MEETING, 2008, Bangkok, Thailand. Poster abstracts... Mexico, DF: Generation Challenge Programme, 2008. p. 54.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | MUTEGI, R.; FERGUSON, M.; MAASS, B. L.; MKAMILO, G.; KAMAU, J.; ADJEBENG-DANQUAH, J.; ALVES, A.; SETTER, T.; FREGENE, M. Field evaluation of cassava varieties under drought stress in Kenya, Tanzania and Ghana. In: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent. Cassava: meeting the challenges of the new millennium. Ghent:: IPBO, 2008. p. 148. SP10-07.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | ALVES, A. A. C.; RODRIGUEZ, M. A. D.; FARIAS, A. R. N.; SILVA, A. F.; BELLOTTI, A.; FREGENE, M. Pré-melhoramento de mandioca: utilização de espécies silvestres de Manihot como fonte de resistência a estresses bióticos e abióticos. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 78.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | ALVES, A. A. C.; RODRIGUEZ, M. A. D.; FARIAS, A. R. N.; SILVA, A. F.; BELLOTTI, A.; FREGENE, M. Pré-melhoramento de mandioca: utilização de espécies silvestres de Manihot como fonte de resistência a estresses bióticos e abióticos. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 78.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
|  |
| 17. | | ALVES, A.; MENDES, R.; CARVALHO, P. C. de; COSTA, I.; DITA, M.; FARIAS, A.; SILVA, A.; TAVARES FILHO, L.; LEDO, C.; FREGENE, M. Cassava wild relatives utilisation at Embrapa. In: CGIAR GENERATION CHALLENGE PROGRAMME ANNUAL RESEARCH MEETING, 2008, Bangkok, Thailand. Poster abstracts... México, DF: Generation Challenge Programme, 2008. p. 52.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 18. | | ALVES, A. A. C.; FREGENE, M.; FERGUSON, M.; SETTER, T.; CEBALLOS, H.; MKAMILO, G.; KANJU, E.; OSEI, C.; SOUZA, A.; RODRÍGUEZ, M. A. D.; SILVA, A. Identifying the physiological and genetic traits that make cassava one of the most drought tolerant crops. In: GENERATION CHALLENGE PROGRAMME. Project mid-year and final reports: competitive and commissioned projects: 2007. Mexico, DF: Generation Challenge Programme, 2007. p. 47-49.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | ALVES, A.; DITA, M.; SOUZA, A.; SILVA, A. F.; FREGENE, M.; CEBALLOS, H.; SETTER, T.; DUQUE, L.; FERGUSON, M.; MUTEGI, R.; MKAMILO, G.; ADJEBENG-DANQUAH, J.; KAMAU, J. Identifying the physiological and genetic traits that make cassava one of the most drought tolerant crops. In: CGIAR GENERATION CHALLENGE PROGRAMME ANNUAL RESEARCH MEETING, 2008, Bangkok, Thailand. Project abstracts... Mexico, DF: Generation Challenge Programme, 2008. p. 30-31.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 19 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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