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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/06/1997 |
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Data da última atualização: |
04/06/1997 |
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Autoria: |
TAYLOR, P. W. J.; GEIJSKES, J. R.; KO, H. L; FRASER, T. A. |
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Afiliação: |
Bureau of Sugar Experiment Stations, Indooroopilly, Queensland-Australia. Universidad of Queensland-Australia. |
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Título: |
Sensitivity of random amplified polymorphic DNA analysis to detedt genetic change in sugarcane during tissue culture |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Theor appl genet, v. 90, p. 1169-1173, 1995 |
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Idioma: |
Inglês |
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Conteúdo: |
Random amplified polymorfic DNA (RAPD) analysis using 10-mer oligonucleotide primers efficiently differentiare sugarcane cultivars and proved suitable for detecting gross genetic change such as that which can occur in sugarcane subjeted to prolonged tissue culture, for example in protoplast-derived calls. However, RAPD analysis was not sufficiently sensitive to detect smaller genetic changes that occur during suarcane genetic transformation. The length of DNA scored for polymorphism per primer averaged 13.2 kb, or 0.0001% of the typical sugarcane genome size of 1.2 x 10 elevado a sete kb (2C). RAPD analysis of sugarcane plants regenerated from embryogenic callus revealed very few polymorphisms, indicating that gross genetic change is infrequent during this tissue culture procedure, although epigenetic effects result in transient morphological changes in regenerated plants. More sensitive variations on the RAPD technique may increase the practicality of DNA-based screening of regenerated plant lines to reveal somaclonal variants. |
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Categoria do assunto: |
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Marc: |
LEADER 01517naa a2200157 a 4500
001 1647891
005 1997-06-04
008 1995 bl --- 0-- u #d
100 1 $aTAYLOR, P. W. J.
245 $aSensitivity of random amplified polymorphic DNA analysis to detedt genetic change in sugarcane during tissue culture
260 $c1995
520 $aRandom amplified polymorfic DNA (RAPD) analysis using 10-mer oligonucleotide primers efficiently differentiare sugarcane cultivars and proved suitable for detecting gross genetic change such as that which can occur in sugarcane subjeted to prolonged tissue culture, for example in protoplast-derived calls. However, RAPD analysis was not sufficiently sensitive to detect smaller genetic changes that occur during suarcane genetic transformation. The length of DNA scored for polymorphism per primer averaged 13.2 kb, or 0.0001% of the typical sugarcane genome size of 1.2 x 10 elevado a sete kb (2C). RAPD analysis of sugarcane plants regenerated from embryogenic callus revealed very few polymorphisms, indicating that gross genetic change is infrequent during this tissue culture procedure, although epigenetic effects result in transient morphological changes in regenerated plants. More sensitive variations on the RAPD technique may increase the practicality of DNA-based screening of regenerated plant lines to reveal somaclonal variants.
700 1 $aGEIJSKES, J. R.
700 1 $aKO, H. L
700 1 $aFRASER, T. A.
773 $tTheor appl genet$gv. 90, p. 1169-1173, 1995
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Embrapa Mandioca e Fruticultura (CNPMF) |
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