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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
31/03/2017 |
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Data da última atualização: |
13/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. de; DEGENHARDT-GOLDBACH, J.; BETTENCOURT, G. M. de F.; AMANO, E.; FRANCISCON, L.; QUOIRIN, M. |
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Afiliação: |
CASSIANA DE OLIVEIRA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; GISELA MANUELA DE FRANÇA BETTENCOURT; ERIKA AMANO, UFPR; LUZIANE FRANCISCON, CNPF; MARGUERITE QUOIRIN, UFPR. |
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Título: |
Micropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Forestry Research, v. 28, n. 1, p. 29-39, Jan. 2017. |
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DOI: |
10.1007/s11676-016-0282-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Genetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %. MenosGenetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, a... Mostrar Tudo |
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Palavras-Chave: |
Callogenesis; Eucalyptus urograndis; WPM culture medium. |
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Thesagro: |
Micropropagação; Organogênese; Propagação vegetativa. |
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Thesaurus Nal: |
Callus formation; Micropropagation; Organogenesis; Plant propagation. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02512naa a2200313 a 4500
001 2067974
005 2017-12-13
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s11676-016-0282-6$2DOI
100 1 $aOLIVEIRA, C. de
245 $aMicropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone.$h[electronic resource]
260 $c2017
520 $aGenetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %.
650 $aCallus formation
650 $aMicropropagation
650 $aOrganogenesis
650 $aPlant propagation
650 $aMicropropagação
650 $aOrganogênese
650 $aPropagação vegetativa
653 $aCallogenesis
653 $aEucalyptus urograndis
653 $aWPM culture medium
700 1 $aDEGENHARDT-GOLDBACH, J.
700 1 $aBETTENCOURT, G. M. de F.
700 1 $aAMANO, E.
700 1 $aFRANCISCON, L.
700 1 $aQUOIRIN, M.
773 $tJournal of Forestry Research$gv. 28, n. 1, p. 29-39, Jan. 2017.
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Embrapa Florestas (CNPF) |
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| Registros recuperados : 103 | |
| 47. |  | MORÉS, R.; CESTONARO, T.; PRÁ, M. C. de; ABREU, P. G. de; FRANCISCON, L.; MAZZUCO, H. Efeito da qualidade da casca e total de poros de ovos em relação ao posicionamento da ave no aviário. In: CONGRESSO DE PRODUÇÃO, COMERCIALIZAÇÃO E CONSUMO DE OVOS, 7., 2009, São Pedro. Avicultura de postura: uma janela para o futuro: anais. São Paulo: APA, 2009. p. 257-259. Projeto/Plano de Ação: 03.06.62.000-03.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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| 51. | | ZANELLA, L. B.; FRANCISCON, L.; GRUNENNVALDT, R. L.; TOMASI, J. de C.; DEGENHARDT-GOLDBACH, J. Micropropagation of Pinus tecunumanii. Ciência Florestal, Santa Maria, v. 28, n. 2, p. 651-660, abr./jun. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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| 52. | | MAZZUCO, H.; FRANCISCON, L.; COLDEBELLA, A.; MORES, R.; AVILA, V. S. de. Molting hens using soy hulls: 1. physiological response through blood metabolites In: ANNUAL MEETING [OF THE] POULTRY SCIENCE ASSOCIATION, 98., 2009, Raleigh. Abstracts. Raleigh: Poultry Science Association, 2009. 1 CD-ROM. Suplemento 1 da revista Poultry Science, v. 99. Projeto/Plano de Ação: 03.06.62.000-01| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 54. | | FRANCISCON, L.; RIBEIRO JUNIOR, P. J.; KRAINSKI, E. T.; BASSANEZI, R. B.; CZERMAINSKI, A. B. C. Modelo autologístico espaço-temporal com aplicação à análise de padrões espaciais da Leprose-dos-citros. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 12, p. 1677-1682, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
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| 55. | | MAEDA, S.; BOGNOLA, I. A.; KURIHARA, C. H.; FRANCISCON, L.; OLIVEIRA, E. B. de. Obtenção de valores de referência do DRIS para Pinus taeda. In: INAMASU, R. Y.; NAIME, J. de M.; RESENDE, A. V. de; BASSOI, L. H.; BERNARDI, A. C. de C. (Ed.). Agricultura de precisão: um novo olhar. São Carlos: Embrapa Instrumentação, 2011. p. 232-234.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Florestas. |
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| 58. | | BOGNOLA, I. A.; CLASEN, L. A.; FRANCISCON, L.; GAVA, J. L.; DEDECEK, R. A.; SILVA, F. M. da. Aplicação de silicatos de cálcio e de potássio e o crescimento de mudas de Eucalyptus grandis. Pesquisa Florestal Brasileira, Colombo, v. 31, n. 66, p. 83-92, abr./jun. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Florestas. |
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| 59. | | ABREU, P. G. D.; SABINO, L. A.; ABREU, V. M. N.; FRANCISCON, L.; COREZZOLA, R. R.; DALLE LASTE, R. J. Análise espacial de instalaçoes de suínos em fase reprodutiva In: CONGRESO ARGENTINO DE INGENIERÍA RURAL, 10.; MERCOSUR, 2., 2009, Rosário . Actas: el compromisso de la ingeniería rural con el desarrollo territorial. Rosário: Editorial de la Universidad Nacional de Rosario, 2009 Projeto/Plano de Ação: 03.07.94.900-02| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 103 | |
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