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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
10/03/2005 |
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Data da última atualização: |
10/03/2005 |
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Autoria: |
FORATO, L. A.; YUSHMANOV, V. E.; COLNAGO, L. A. |
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Título: |
Interaction of two prolamins with 1- 13C oleic acid by 13C NMR. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Biochemistry, v. 43, p. 7121-7126, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: In this paper, we analyzed the interaction of Z 19 prolamin from a BR451 maize variety and pennisetin from a BRS1501 pearl millet variety with 1-13C-enriched oleic acid (OA) by 13C NMR in solution. In both proteins, we identified the presence of free fatty acids by NMR in solid state and solution. The interactions were analyzed at the protein/OA molar ratios of 1:1 and 1:4. In the Zl9/0A 1:1 mixture in 70% ethanol and 30% D2O, the chemical shift of OA CI was 182.9 ppm, about 3 ppm above that of the pure OA in the same solvent. In contrast, upon addition of OA to the pennisetin (1: 1), the chemical- shift values slightly decreased by less than 1 ppm. The chemical-shift titration curve of OA C1 in an apparent pH range of 5.5-7.3 shifted by approximately 0.3 pH units toward higher pH values in the pennisetin/OA 1:1 complex relative to the pure OA. The results obtained for the pennisetin/OA 1:4 mixture were similar to the complexes at a 1:1 molar ratio. A significant difference was observed between the I: 1 and 1:4 curves for Z19. The titration curve for Z19/0A 1:1 suggested specific binding at the sites with electrostatic interaction.
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Palavras-Chave: |
Prolamina. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01576naa a2200157 a 4500
001 1028841
005 2005-03-10
008 2004 bl --- 0-- u #d
100 1 $aFORATO, L. A.
245 $aInteraction of two prolamins with 1- 13C oleic acid by 13C NMR.
260 $c2004
520 $aABSTRACT: In this paper, we analyzed the interaction of Z 19 prolamin from a BR451 maize variety and pennisetin from a BRS1501 pearl millet variety with 1-13C-enriched oleic acid (OA) by 13C NMR in solution. In both proteins, we identified the presence of free fatty acids by NMR in solid state and solution. The interactions were analyzed at the protein/OA molar ratios of 1:1 and 1:4. In the Zl9/0A 1:1 mixture in 70% ethanol and 30% D2O, the chemical shift of OA CI was 182.9 ppm, about 3 ppm above that of the pure OA in the same solvent. In contrast, upon addition of OA to the pennisetin (1: 1), the chemical- shift values slightly decreased by less than 1 ppm. The chemical-shift titration curve of OA C1 in an apparent pH range of 5.5-7.3 shifted by approximately 0.3 pH units toward higher pH values in the pennisetin/OA 1:1 complex relative to the pure OA. The results obtained for the pennisetin/OA 1:4 mixture were similar to the complexes at a 1:1 molar ratio. A significant difference was observed between the I: 1 and 1:4 curves for Z19. The titration curve for Z19/0A 1:1 suggested specific binding at the sites with electrostatic interaction.
653 $aProlamina
700 1 $aYUSHMANOV, V. E.
700 1 $aCOLNAGO, L. A.
773 $tBiochemistry$gv. 43, p. 7121-7126, 2004.
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Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 219 | |
| 1. |  | FORATO, L. A. Biopolymers for edible food coatings. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE – SIRGEALC, 11., 2009, Pucón, Chile. Proceeding... Pucón, Chile: INIA, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 3. | | MIRANDA, M. M. P.; FORATO, L. A. Desenvolvimento de sonda de RMN com bobinas acopladas indutivamente. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 6., 2014, São Carlos, SP Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2014. p. 24. Editores técnicos: João de Mendonça Naime, Caue Ribeiro, Maria Alice Martins, Elaine Cristina Paris, Paulino Ribeiro Villas Boas, Ladislau Marcelino Rabello. (Embrapa Instrumentação. Documentos, 57).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | ASSIS, O. B. G.; FORATO, L. A. Biopolymers for edible food coatings. In: ENCONTRO DA SOCIEDADE BRASILEIRA DE PESQUISA EM MATERIAIS - SBPMat, 7., 2008, Guarujá; BRAZILIAN MRS MEETING, 7., 2008, Guarujá. Abstracts... Rio de Janeiro: SBPMat, 2008. não paginado. 1CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 219 | |
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