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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
Data corrente: |
08/10/2013 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SIQUEIRA, L. G. B.; AREAS, V. S.; GHETTI, A. M.; FONSECA, J. F. da; PALHAO, M. P.; FERNANDES, C. A. C.; VIANA, J. H. M. |
Afiliação: |
LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; V. S. AREAS, UFES; A. M. GHETTI, UFF; JEFERSON FERREIRA DA FONSECA, CNPC; M. P. PALHAO, UJRV, Alfenas, MG; C. A. C. FERNANDES, UJRV, Alfenas MG; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
Título: |
Color Doppler flow imaging for the early detection of nonpregnant cattle at 20 days after timed artificial insemination. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Journal of Dairy Science, v. 96, n. 10, p. 6461-6472, 2013. |
DOI: |
https://doi.org/10.3168/jds.2013-6814 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The objective was to determine the accuracy of a pregnancy test for predicting nonpregnant cattle based on the evaluation of corpus luteum (CL) blood flow at 20 d (CLBF-d20) after timed artificial insemination (TAI). Crossbred Holstein-Gir dairy heifers (n = 209) and lactating cows (n = 317) were synchronized for TAI using the following protocol: intravaginal implant (1.0 g of progesterone) and 2 mg of estradiol benzoate i.m. on d −10, implant removal and 0.526 mg of sodium cloprostenol i.m. on d −2, 1 mg of estradiol benzoate i.m. on d −1, and TAI on d 0. On d 20, animals underwent grayscale ultrasonography (US) to locate the CL and color flow Doppler to evaluate CLBF-d20 using a portable ultrasound equipped with a 7.5-MHz rectal transducer. Based only on a visual, subjective CLBF evaluation, the animals were classified as pregnant or not pregnant. On d 30 to 35, blinded from results of the previous diagnosis, the same operator performed a final pregnancy diagnosis using US to visualize the fetal heartbeat (gold standard; US-d30). A second evaluator also analyzed the CLBF-d20 in the same animals by watching 7-s recorded videos. Blood samples were collected from a subset of 171 females to determine, by RIA, plasma progesterone (P4) concentrations, which indicate CL function. The final pregnancy outcome (US-d30) was retrospectively compared with the CLBFd20 diagnoses and then classified either as correct or incorrect. The number of true positive, true negative, false positive, and false negative decisions were inserted into a 2 × 2 decision matrix. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the CLBF-d20 test were calculated using specific equations. Binomial variables (pregnancy rate and proportions) were analyzed using Fisher?s exact test for the effect of parity and to compare between evaluators and tests (CLBF-d20 vs. plasma P4). The kappa values were calculated to quantify the agreement between CLBF-d20 and the gold standard (US-d30) and between evaluators. The performance parameters of CLBF-d20 test were as follows: sensitivity = 99.0%, specificity = 53.7%, positive predictive value = 65.1%, negative predictive value = 98.5%, and accuracy = 74.8%. False negatives represented only 0.4% of the exams. No differences existed in these parameters between evaluators (no. 1 vs. no. 2) and tests (CLBF-d20 vs. plasma P4). Moreover, a high level of agreement was observed between evaluators (0.91). In conclusion, visual evaluation of CLBF-d20 represents a quick, reliable, and consistent diagnostic test that enables the early detection of nonpregnant cattle. MenosAbstract: The objective was to determine the accuracy of a pregnancy test for predicting nonpregnant cattle based on the evaluation of corpus luteum (CL) blood flow at 20 d (CLBF-d20) after timed artificial insemination (TAI). Crossbred Holstein-Gir dairy heifers (n = 209) and lactating cows (n = 317) were synchronized for TAI using the following protocol: intravaginal implant (1.0 g of progesterone) and 2 mg of estradiol benzoate i.m. on d −10, implant removal and 0.526 mg of sodium cloprostenol i.m. on d −2, 1 mg of estradiol benzoate i.m. on d −1, and TAI on d 0. On d 20, animals underwent grayscale ultrasonography (US) to locate the CL and color flow Doppler to evaluate CLBF-d20 using a portable ultrasound equipped with a 7.5-MHz rectal transducer. Based only on a visual, subjective CLBF evaluation, the animals were classified as pregnant or not pregnant. On d 30 to 35, blinded from results of the previous diagnosis, the same operator performed a final pregnancy diagnosis using US to visualize the fetal heartbeat (gold standard; US-d30). A second evaluator also analyzed the CLBF-d20 in the same animals by watching 7-s recorded videos. Blood samples were collected from a subset of 171 females to determine, by RIA, plasma progesterone (P4) concentrations, which indicate CL function. The final pregnancy outcome (US-d30) was retrospectively compared with the CLBFd20 diagnoses and then classified either as correct or incorrect. The number of true positive, t... Mostrar Tudo |
Palavras-Chave: |
Bovines; Doppler; Luteólise; Ultra-Som; Ultrassonografia. |
Thesagro: |
Bovino; Circulação Sangüínea; Corpo lúteo; Diagnóstico; Gado; Inseminação artificial; Prenhez; Reprodução animal; Vaca. |
Thesaurus Nal: |
Animal reproduction; Blood circulation; blood flow; Cattle; corpus luteum; Luteolysis; Pregnancy. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03964naa a2200457 a 4500 001 1980679 005 2024-02-05 008 2013 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3168/jds.2013-6814$2DOI 100 1 $aSIQUEIRA, L. G. B. 245 $aColor Doppler flow imaging for the early detection of nonpregnant cattle at 20 days after timed artificial insemination.$h[electronic resource] 260 $c2013 520 $aAbstract: The objective was to determine the accuracy of a pregnancy test for predicting nonpregnant cattle based on the evaluation of corpus luteum (CL) blood flow at 20 d (CLBF-d20) after timed artificial insemination (TAI). Crossbred Holstein-Gir dairy heifers (n = 209) and lactating cows (n = 317) were synchronized for TAI using the following protocol: intravaginal implant (1.0 g of progesterone) and 2 mg of estradiol benzoate i.m. on d −10, implant removal and 0.526 mg of sodium cloprostenol i.m. on d −2, 1 mg of estradiol benzoate i.m. on d −1, and TAI on d 0. On d 20, animals underwent grayscale ultrasonography (US) to locate the CL and color flow Doppler to evaluate CLBF-d20 using a portable ultrasound equipped with a 7.5-MHz rectal transducer. Based only on a visual, subjective CLBF evaluation, the animals were classified as pregnant or not pregnant. On d 30 to 35, blinded from results of the previous diagnosis, the same operator performed a final pregnancy diagnosis using US to visualize the fetal heartbeat (gold standard; US-d30). A second evaluator also analyzed the CLBF-d20 in the same animals by watching 7-s recorded videos. Blood samples were collected from a subset of 171 females to determine, by RIA, plasma progesterone (P4) concentrations, which indicate CL function. The final pregnancy outcome (US-d30) was retrospectively compared with the CLBFd20 diagnoses and then classified either as correct or incorrect. The number of true positive, true negative, false positive, and false negative decisions were inserted into a 2 × 2 decision matrix. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the CLBF-d20 test were calculated using specific equations. Binomial variables (pregnancy rate and proportions) were analyzed using Fisher?s exact test for the effect of parity and to compare between evaluators and tests (CLBF-d20 vs. plasma P4). The kappa values were calculated to quantify the agreement between CLBF-d20 and the gold standard (US-d30) and between evaluators. The performance parameters of CLBF-d20 test were as follows: sensitivity = 99.0%, specificity = 53.7%, positive predictive value = 65.1%, negative predictive value = 98.5%, and accuracy = 74.8%. False negatives represented only 0.4% of the exams. No differences existed in these parameters between evaluators (no. 1 vs. no. 2) and tests (CLBF-d20 vs. plasma P4). Moreover, a high level of agreement was observed between evaluators (0.91). In conclusion, visual evaluation of CLBF-d20 represents a quick, reliable, and consistent diagnostic test that enables the early detection of nonpregnant cattle. 650 $aAnimal reproduction 650 $aBlood circulation 650 $ablood flow 650 $aCattle 650 $acorpus luteum 650 $aLuteolysis 650 $aPregnancy 650 $aBovino 650 $aCirculação Sangüínea 650 $aCorpo lúteo 650 $aDiagnóstico 650 $aGado 650 $aInseminação artificial 650 $aPrenhez 650 $aReprodução animal 650 $aVaca 653 $aBovines 653 $aDoppler 653 $aLuteólise 653 $aUltra-Som 653 $aUltrassonografia 700 1 $aAREAS, V. S. 700 1 $aGHETTI, A. M. 700 1 $aFONSECA, J. F. da 700 1 $aPALHAO, M. P. 700 1 $aFERNANDES, C. A. C. 700 1 $aVIANA, J. H. M. 773 $tJournal of Dairy Science$gv. 96, n. 10, p. 6461-6472, 2013.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
27/06/1996 |
Data da última atualização: |
04/05/2017 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
ALBUQUERQUE, T. C. S. de; ALBUQUERQUE, J. A. S. de. |
Afiliação: |
TERESINHA COSTA SILVEIRA DE ALBUQUERQUE, CPATSA; JOÃO ANTONIO SILVA DE ALBUQUERQUE, CPATSA. |
Título: |
Enxertia de mesa em videira no Submédio São Francisco. |
Ano de publicação: |
1984 |
Fonte/Imprenta: |
Petrolina: EMBRAPA-CPATSA, 1984. |
Páginas: |
6 p. |
Descrição Física: |
il. |
Série: |
(EMBRAPA-CPATSA. Comunicado técnico, 12). |
Idioma: |
Português |
Conteúdo: |
Constatou-se excelentes resultados com a enxertia de mesa, realizada no Campo Experimental de Mandacaru. Este método de enxertia apresenta uma série de vantagens. São apresentados os seguintes ítens, para a realização deste método: preparo dos enxertos; preparo dos porta-enxertos; enxertia; plantio da estaca enxertada e transplantio da muda para o campo. |
Palavras-Chave: |
Brasil; Graft; Grape; Nordeste; Northeast; Submédio São Francisco. |
Thesagro: |
Enxerto; Uva. |
Thesaurus NAL: |
Brazil; grapes. |
Categoria do assunto: |
-- A Sistemas de Cultivo |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA/6650/1/COT12.pdf
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Marc: |
LEADER 01039nam a2200265 a 4500 001 1131410 005 2017-05-04 008 1984 bl uuuu u0uu1 u #d 100 1 $aALBUQUERQUE, T. C. S. de 245 $aEnxertia de mesa em videira no Submédio São Francisco. 260 $aPetrolina: EMBRAPA-CPATSA$c1984 300 $a6 p.$cil. 490 $a(EMBRAPA-CPATSA. Comunicado técnico, 12). 520 $aConstatou-se excelentes resultados com a enxertia de mesa, realizada no Campo Experimental de Mandacaru. Este método de enxertia apresenta uma série de vantagens. São apresentados os seguintes ítens, para a realização deste método: preparo dos enxertos; preparo dos porta-enxertos; enxertia; plantio da estaca enxertada e transplantio da muda para o campo. 650 $aBrazil 650 $agrapes 650 $aEnxerto 650 $aUva 653 $aBrasil 653 $aGraft 653 $aGrape 653 $aNordeste 653 $aNortheast 653 $aSubmédio São Francisco 700 1 $aALBUQUERQUE, J. A. S. de
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