| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
14/01/2020 |
|
Data da última atualização: |
06/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. |
|
Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; Ribrio Ivan Tavares Pereira Batista; Juliane Teramachi Trevizan; Joanna Maria Gonçalves Souza-Fabjan; Felipe Zandonadi Brandão; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
|
Título: |
Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019. |
|
DOI: |
10.5433/1679-0359.2019v40n6Supl3p3789 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation. MenosAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that trans... Mostrar Tudo |
|
Palavras-Chave: |
Bovine oocytes; COCs; Ex-situ maturation; In vitro maturation; Nonsurgical embryo transfer technique. |
|
Thesaurus Nal: |
Goats. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208736/1/Goat-incubator-can-bovine-oocytes.pdf
|
|
Marc: |
LEADER 02452naa a2200265 a 4500
001 2118709
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2019v40n6Supl3p3789$2DOI
100 1 $aFONSECA, J. F. da
245 $aGoat incubator$bcan bovine oocytes be matured in the uterine horn of a goat?$h[electronic resource]
260 $c2019
520 $aAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation.
650 $aGoats
653 $aBovine oocytes
653 $aCOCs
653 $aEx-situ maturation
653 $aIn vitro maturation
653 $aNonsurgical embryo transfer technique
700 1 $aBATISTA, R. I. T. P.
700 1 $aTREVIZAN, J. T.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aBRANDÃO, F. Z.
700 1 $aCAMARGO, L. S. de A.
773 $tSemina: Ciências Agrárias, Londrina$gv. 40, n. 6, supp. 3, p. 3789-3796, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 533 | |
| 141. |  | MAFFILI, V. V.; TORRES, C. A. A.; FONSECA, J. F. da; BRUSCHI, J. H.; VIANA, J. H. M.; PROSPERI, C. P.; MORAES, E. A.; PONTES, R. A. M. Eficiência do CIDR novo e reutilizado em protocolo de sincronização do estro de curta duração em cabras da raça Toggenburg. Acta Scientiae Veterinariae, v. 33, supl. 1, p. s252, 2005. Edição dos resumos da 19a. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Angra dos Reis, RJ, ago., 2005.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
|    |
| 142. | | FONSECA, J. F. da; ZAMBRINI, F. N.; ALVIM, G. P.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; VIANA, J. H. M. Embryo production and recovery in goats by non-surgical transcervical technique. Small Ruminant Research, v. 111, n. 1/3, p. 96-99, Apr. 2013.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
|  |
| 143. | | FONSECA, J. F. da; SILVA, A. L. S.; NOGUEIRA, L. A. G.; CAVALCANTI, A. S.; BARTZ, C.; MOTA, M. F.; BRANDÃO, F. Z. Efeitos do uso de Dinoprost sobre a sincronização do estro e concentração plasmática de progesterona (P4) em ovelhas entre o quarto e sexto dia do ciclo estral em ovelhas. Acta Scientiae Veterinariae, Porto Alegre, v. 35, p. s1281, 2007. Suplemento 3. Edição dos anais da 21o. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões - SBTE, Salvador, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 144. | | VENTURI, S. S.; FONSECA, J. F. da; RIBEIRO, A. C. S.; ROGERIO, M. C. P.; OLIVEIRA, J.; ARAÚJO, A. R.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z. Energy supplementation in Santa Inês sheep subjected to estrus induction treatment. Semina: Ciências Agrárias, Londrina, v. 37, n. 1, p. 145-154, jan./fev. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 145. | | MAIA, A. L. R. S.; SILVA, M. R.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; FARIA, L. S.; CÔRTES, L. R.; FACO, O.; FONSECA, J. F. da. Epidemiological survey and risk factors associated with hydrometra in dairy goat herds. Small Ruminant Research, v. 178, p. 79-84, Sept. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
| |
| 146. | | CARNEIRO, C.; SOUZA, J. M. G.; TORRES, C. A. C.; SILVA, W. J.; DENADAI, R.; BRUSCHI, J. H.; FONSECA, J. F. da. Effects of bypass lipid supplementation in the transition period on reproductive parameters in dairy goats after parturition. Reproduction, Fertility and Development, v. 22, n. 1, p. 377?378, 2010. Edição dos Proceedings of the Annual Conference of the International Embryo Transfer Society, Córdoba, Argentina, 9?12 January 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 147. | | CARNEIRO, C.; SOUZA, J. M. G.; TORRES, C. A. A.; SILVA, W. J.; DENADAI, R.; BRUSCHI, J. H.; FONSECA, J. F. da. Effects of bypass lipid supplementation in the transition period on reproductive parameters in dairy goats after parturition. Reproduction, Fertility and Development, v. 22, n. 1, 2010. Edição dos proceedings do Annual Conference of the International Embryo, 2010, Cordoba.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 148. | | CARVALHO-DE-PAULA, C. J.; SOUZA-FABJAN, J. M. G.; GONÇALVES, J. D.; DIAS, J. H.; SOUZA, G. N. de; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Effect of a 12-h increment in the short-term treatment regimen on ovarian status, estrus synchrony, and pregnancy rate in artificially inseminated dairy goats. Animal Reproduction Science, v. 221, e106571, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
| |
| 149. | | AMORIM, L. S.; TORRES, C. A. A.; AMORIM, E. A. M.; GUIMARÃES, J. D.; FONSECA, J. F. da; SIQUEIRA, L. G. B.; SEIDEL, G. E. Effect of dietary urea on embryonic viability and development of Toggenburg goats. Reproduction, Fertility and Development, v.21, n. 1, p. 151, 2009. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, San Diego, California, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 150. | | ANDRADE, A. B. P. de; MORAIS, M. C. C.; RANGELM P. S. C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Effect of eCG in a short-term synchronization treatment on ovarian status, estrus synchrony, and ovulation in dairy goats managed under tropical conditions. Tropical Animal Health and Production, v. 53, n. 246, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 151. | | MACIEL, G.; RODRIGUEZ, M.; MARONEZ, M.; CAMPOS, G.; SILVA, P.; TREVIZAN, J.; USCATEGUI, R.; FONSECA, J. F. da; FELICIANO, M.; VICENTE, V.; OLIVEIRA, M. E. Effect of the FSH dose on superovulatory response in Santa Inês ewes. Reproduction and Domestic Animal, v. 51, n. S2, p. 112. 2016. Abstract P144. Proceedings of the 20th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR) and the 13th Conference of the Spanish Association for Animal Reproduction (AERA), Lisbon, Portugal, 27?29 October 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 152. | | OLIVEIRA, M. E. F.; SANTOS, I. C. C.; VICENTE, W. W. R.; CORDEIRO, M. F.; FONSECA, J. F. da; SOUZA, S. F. Effects of GnRH in synchronization protocol on ovarian responses in Santa Inês ewes. Reproduction in Domestic Animal, v. 43, supl. 5, 2008. Abstract P73. Edição dos Abstracts do Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR), 12., 2008, Gent. Doi: 10.1111/j.1439-0531.2008.01256.x| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 153. | | FONSECA, J. F. da; MAFFILI, V. V.; RODRIGUES, M. T.; SANTOS, A. D. F.; ROVAY, H.; PINTO NETO, A.; BRANDÃO, F. Z.; TORES, C. A. A. Effects of hCG on progesterone concentrations and fertility in cyclic, lactating Alpine goats. Animal Reproduction, v. 3, n. 4, p. 410-414, Oct./Dec. 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 154. | | FONSECA, J. F. da; MAFFILI, V. V.; SANTOS, A. D. F.; FÜRST, R.; PROSPERI, C. P.; ROVAY, H.; TORRES, C. A. A. Effects of prostaglandin administration 10 days apart on estrus, ovulation and pregnancy in nulliparaous dairy goats. In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23., 2008, Querétaro, México. Sustainable goat production: challenges and opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008. p. 239. ref. 340| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 155. | | FONSECA, J. F. da; MAFFILI, V. V.; SANTOS, A. D. F.; FÜRST, R.; PROSPERI, C. P.; SOUZA, J. M. G.; TORRES, C. A. A. Effects of prostaglandin administration 10 days apart on reproductive parameters of cyclic dairy nulliparous goats. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 64, n. 2, p. 349-358, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 156. | | TEIXEIRA, T. A.; FONSECA, J. F. da; SOUZA-FABJAN, J. M. G. de; CARVALHEIRA, L. de R.; FERNANDES, D. A. de M.; BRANDÃO, F. Z. Efficiency of different hormonal treatments for estrus synchronization in tropical Santa Inês sheep. Tropical Animal Health and Production, Heidelberg, v. 48, n. 3, p. 545-551, Mar. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 157. | | FIGUEIRA, L.; SOUZA-FABJAN, J.; BATISTA, R.; SOUZA, L.; DIÓGENES, Y.; FILGUEIRAS, M.; SOUZA, G.; ALVES, N.; FONSECA, J. F. da. Efficient transcervical embryo collection in synchronous estrus-induced Lacaune ewes. Animal Reproduction, v. 15, p. 1121. 2018. Suplemento 1. Abstract 107. Edição dos Proceedings of the 10th International Ruminant Reproduction Symposium (IRRS), Foz do Iguaçu, PR, Brazil, September 16th to 20th, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 158. | | FONSECA, J. F. da; SOUZA, J. M. G. de; RIBEIRO, A. C.; RIBEIRO, S. D. A.; VIANA, J. H. M.; FACO, O. Estrus and fertility of anestrous Anglo-Nubian goats submitted to different synchronous protocols and given hCG five days after artificial insemination. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 18., 2009, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2009.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 159. | | FONSECA, J. F. da; SOUZA, J. M. G.; RIBEIRO, A. C.; RIBEIRO, S. D. A.; VIANA, J. H. M.; FACO, O. Estrus and fertility of anestrous Anglo-Nubian goats submitted to different synchronous protocols and given hCG five days after artificial insemination. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 18., 2009, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2009. p. 408. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 160. | | MAIA, A. L. R. e S.; SOUZA-FABJAN, J. M. G. de; RANGEL, P. S. C.; CÔRTES, L. R.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Estrus induction in the non-breeding season is not associated with hydrometra in dairy goats. Research, Society and Development, v. 10, n. 11, e21101119162, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| Registros recuperados : 533 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
