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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
10/12/2019 |
Data da última atualização: |
10/12/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OLIVEIRA, M. E. F.; FONSECA, J. F. da; VICENTE, W. R. R.; RODRIGUES, N. N.; VERGANI, G. B.; SOUZA-FABJAN, J. M. G.; JAMIESON, M.; CRISTESCU, A.; MURAWSKI, M.; BARTLEWSKI, P. M. |
Afiliação: |
MARIA EMILIA FRANCO OLIVEIRA; JEFERSON FERREIRA DA FONSECA, CNPC; WILTER RICARDO RUSSIANO VICENTE; NAIARA NANTES RODRIGUES; GABRIEL BRUN VERGANI; JOANNA MARIA GONÇALVES SOUZA-FABJAN; MARK JAMIESON; ADA CRISTESCU; MACIEJ MURAWSKI; PAWEL MIECZYSLAW BARTLEWSKI. |
Título: |
Are the spectral Doppler indices of ovarian arteries indicative of antral follicular development and predictive of ovulatory responses and embryo yields in superovulated ewes?. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Reproductive Biology, v. 19, p. 394-403, 2019. |
DOI: |
10.1016/j.repbio.2019.11.004 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Nineteen ewes received 200 mg of pFSH administered in eight decreasing doses from Days 1 to 4, starting three days before CIDR® device removal. Ten ewes received an injection of 350 ?g of estradiol benzoate at CIDR® device insertion (Group E) and nine animals served as controls (Group C). B-mode and spectral Doppler ultrasonographic examinations were performed daily throughout superovulatory treatment to enumerate ovarian antral follicles and to determine ovarian blood flow indices, respectively. There were no differences (P > 0.05) in superovulatory responses between left and right ovaries/uterine horns or the two groups of animals. End-diastolic velocity (EDV) and mean velocity (Vm) values were greater (P < 0.05) on Days 1 and 2, and peak systolic velocity (SVp) was greater (P < 0.05) on Day 3 in Group C than in Group E. In Group E 15 correlations was recorded among indices (SVp, Vm, EDV, flow velocity integral-FVI, and pulsatility index-PI) and follicles numbers in different size classes on Days 1, 2 and 4, and seven correlations among indices (SVp, EDV, Vm, and vascular resistance index-RI) and superovulatory/embryo results (numbers of regressing corpora lutea, numbers/percentages of degenerated embryos and viability rates) on Days 1, 2 and 3. In Group C, there were three correlations among EDV and RI and medium-sized/large follicle numbers on Days 1 and 3, and five correlations among indices (EDV, RI and PI) and superovulatory/embryo results (numbers of luteinized unovulated follicles, degenerated embryos and unfertilized eggs) on Days 2 or 4. There was a lack of consistency in the velocimetric correlates of antral follicle numbers and superovulatory responses between the left and right side. Therefore, the usefulness of ovarian arterial indices to predict ovine superovulatory outcomes remains equivocal and requires further confirmatory studies. MenosAbstract: Nineteen ewes received 200 mg of pFSH administered in eight decreasing doses from Days 1 to 4, starting three days before CIDR® device removal. Ten ewes received an injection of 350 ?g of estradiol benzoate at CIDR® device insertion (Group E) and nine animals served as controls (Group C). B-mode and spectral Doppler ultrasonographic examinations were performed daily throughout superovulatory treatment to enumerate ovarian antral follicles and to determine ovarian blood flow indices, respectively. There were no differences (P > 0.05) in superovulatory responses between left and right ovaries/uterine horns or the two groups of animals. End-diastolic velocity (EDV) and mean velocity (Vm) values were greater (P < 0.05) on Days 1 and 2, and peak systolic velocity (SVp) was greater (P < 0.05) on Day 3 in Group C than in Group E. In Group E 15 correlations was recorded among indices (SVp, Vm, EDV, flow velocity integral-FVI, and pulsatility index-PI) and follicles numbers in different size classes on Days 1, 2 and 4, and seven correlations among indices (SVp, EDV, Vm, and vascular resistance index-RI) and superovulatory/embryo results (numbers of regressing corpora lutea, numbers/percentages of degenerated embryos and viability rates) on Days 1, 2 and 3. In Group C, there were three correlations among EDV and RI and medium-sized/large follicle numbers on Days 1 and 3, and five correlations among indices (EDV, RI and PI) and superovulatory/embryo results (numbers of lutein... Mostrar Tudo |
Palavras-Chave: |
Doppler ultrasonography; Estradiol benzoate; Ovarian artery. |
Thesaurus Nal: |
Sheep; Superovulation. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02876naa a2200301 a 4500 001 2116547 005 2019-12-10 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1016/j.repbio.2019.11.004$2DOI 100 1 $aOLIVEIRA, M. E. F. 245 $aAre the spectral Doppler indices of ovarian arteries indicative of antral follicular development and predictive of ovulatory responses and embryo yields in superovulated ewes?.$h[electronic resource] 260 $c2019 520 $aAbstract: Nineteen ewes received 200 mg of pFSH administered in eight decreasing doses from Days 1 to 4, starting three days before CIDR® device removal. Ten ewes received an injection of 350 ?g of estradiol benzoate at CIDR® device insertion (Group E) and nine animals served as controls (Group C). B-mode and spectral Doppler ultrasonographic examinations were performed daily throughout superovulatory treatment to enumerate ovarian antral follicles and to determine ovarian blood flow indices, respectively. There were no differences (P > 0.05) in superovulatory responses between left and right ovaries/uterine horns or the two groups of animals. End-diastolic velocity (EDV) and mean velocity (Vm) values were greater (P < 0.05) on Days 1 and 2, and peak systolic velocity (SVp) was greater (P < 0.05) on Day 3 in Group C than in Group E. In Group E 15 correlations was recorded among indices (SVp, Vm, EDV, flow velocity integral-FVI, and pulsatility index-PI) and follicles numbers in different size classes on Days 1, 2 and 4, and seven correlations among indices (SVp, EDV, Vm, and vascular resistance index-RI) and superovulatory/embryo results (numbers of regressing corpora lutea, numbers/percentages of degenerated embryos and viability rates) on Days 1, 2 and 3. In Group C, there were three correlations among EDV and RI and medium-sized/large follicle numbers on Days 1 and 3, and five correlations among indices (EDV, RI and PI) and superovulatory/embryo results (numbers of luteinized unovulated follicles, degenerated embryos and unfertilized eggs) on Days 2 or 4. There was a lack of consistency in the velocimetric correlates of antral follicle numbers and superovulatory responses between the left and right side. Therefore, the usefulness of ovarian arterial indices to predict ovine superovulatory outcomes remains equivocal and requires further confirmatory studies. 650 $aSheep 650 $aSuperovulation 653 $aDoppler ultrasonography 653 $aEstradiol benzoate 653 $aOvarian artery 700 1 $aFONSECA, J. F. da 700 1 $aVICENTE, W. R. R. 700 1 $aRODRIGUES, N. N. 700 1 $aVERGANI, G. B. 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aJAMIESON, M. 700 1 $aCRISTESCU, A. 700 1 $aMURAWSKI, M. 700 1 $aBARTLEWSKI, P. M. 773 $tReproductive Biology$gv. 19, p. 394-403, 2019.
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Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
30/06/2010 |
Data da última atualização: |
18/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. |
Afiliação: |
CHRISTINA RAMIRES FERREIRA, UNESP-JABOTICABAL; JORG PATRICK BURGSTALLER, UNIVERSTIY OF VETERINARY MEDICINE - VIENNA; FELIPE PERECIN, UNESP - JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP - JABOTICABAL; MARCOS ROBERTO CHIARATTI, USP - PIRASSUNUNGA; SIMONE CRISTINA MÉO, CPPSE; MATHIAS MULLER, UNIVERSITY OF VETERINARY MEDICINE - VIENNA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL - QUEBEC; FLÁVIO VIEIRA MEIRELLES, USP - PIRASSUNUNGA; RALF STEINBORN, UNIVERSITY OF VETERINARY MEDICINE - VIENNA. |
Título: |
Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010. |
DOI: |
https://doi.org/10.1095/biolreprod.109.080564 |
Idioma: |
Inglês |
Conteúdo: |
Ooplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype. |
Palavras-Chave: |
Bovine; Developmental biology; Embryo; Gamete biology; Ooplasmic. |
Thesagro: |
DNA. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/28805/1/PROCISCMN2010.00002.pdf
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Marc: |
LEADER 02370naa a2200313 a 4500 001 1856388 005 2022-11-18 008 2010 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1095/biolreprod.109.080564$2DOI 100 1 $aFERREIRA, C. R. 245 $aPronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line.$h[electronic resource] 260 $c2010 520 $aOoplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype. 650 $aDNA 653 $aBovine 653 $aDevelopmental biology 653 $aEmbryo 653 $aGamete biology 653 $aOoplasmic 700 1 $aBURGSTALLER, J. P. 700 1 $aPERECIN, F. 700 1 $aGARCIA, J. M. 700 1 $aCHIARATTI, M. R. 700 1 $aMÉO, S. C. 700 1 $aMULLER, M. 700 1 $aSMITH, L. C. 700 1 $aMEIRELLES, F. V. 700 1 $aSTEINBORN, R. 773 $tBiology of Reproduction$gv. 82, n. 03, p. 563-571, mar. 2010.
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