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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Data corrente: |
08/02/2019 |
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Data da última atualização: |
04/02/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, J. F. da; ZAMBRINI, F. N.; GUIMARÃES, J. D.; SILVA, M. R.; OLIVEIRA, M. E. F.; BRANDÃO, F. Z.; BARTLEWSKI, P. M.; SOUZA-FABJAN, J. M. G. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; FABIANA NUNES ZAMBRINI, Universidade Federal de Viçosa (UFV) - Viçosa, MG, Brazil; JOSÉ DOMINGOS GUIMARÃES, Universidade Federal de Viçosa (UFV) - Viçosa, MG, Brazil; MARCIO ROBERTO SILVA, CNPGL; MARIA EMILIA FRANCO OLIVEIRA, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brazil; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; PAWEL MIECZYSLAW BARTLEWSKI, University of Guelph, Guelph, Ontario, Canada; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
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Título: |
Combined treatment with oestradiol benzoate, d-cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 54, n. 1, p. 118-125, Sept. 2019. |
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DOI: |
http://dx.doi.org/10.1111/rda.13318 |
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Idioma: |
Inglês |
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Conteúdo: |
Contents This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7‐day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d‐cloprostenol by latero‐vulvar route 7 days apart. After the second injection of d‐cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d‐cloprostenol (latero‐vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d‐cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous‐synchronized Santa Inês ewes pretreated with d‐cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route. MenosContents This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7‐day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d‐cloprostenol by latero‐vulvar route 7 days apart. After the second injection of d‐cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d‐cloprostenol (latero‐vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d‐cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., tr... Mostrar Tudo |
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Palavras-Chave: |
Cervical dilation; Drugs effects; Oestradiol benzoate; Synchronized females; Transcervical embryo collection. |
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Thesagro: |
Estradiol; Hormônio; Ovino; Reprodução Animal; Transferência de Embrião. |
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Thesaurus Nal: |
Cloprostenol; Oxytocin; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02929naa a2200373 a 4500
001 2105726
005 2020-02-04
008 2019 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1111/rda.13318$2DOI
100 1 $aFONSECA, J. F. da
245 $aCombined treatment with oestradiol benzoate, d-cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes.$h[electronic resource]
260 $c2019
520 $aContents This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7‐day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d‐cloprostenol by latero‐vulvar route 7 days apart. After the second injection of d‐cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d‐cloprostenol (latero‐vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d‐cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous‐synchronized Santa Inês ewes pretreated with d‐cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route.
650 $aCloprostenol
650 $aOxytocin
650 $aSheep
650 $aEstradiol
650 $aHormônio
650 $aOvino
650 $aReprodução Animal
650 $aTransferência de Embrião
653 $aCervical dilation
653 $aDrugs effects
653 $aOestradiol benzoate
653 $aSynchronized females
653 $aTranscervical embryo collection
700 1 $aZAMBRINI, F. N.
700 1 $aGUIMARÃES, J. D.
700 1 $aSILVA, M. R.
700 1 $aOLIVEIRA, M. E. F.
700 1 $aBRANDÃO, F. Z.
700 1 $aBARTLEWSKI, P. M.
700 1 $aSOUZA-FABJAN, J. M. G.
773 $tReproduction in Domestic Animals$gv. 54, n. 1, p. 118-125, Sept. 2019.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
18/11/2004 |
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Data da última atualização: |
18/11/2004 |
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Autoria: |
SILVA, C. S.; NICOLAU PINTO, M.; SILVA, J. B. C. da. |
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Afiliação: |
Embrapa Hortaliças, Brasília, DF. |
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Título: |
Uniformidade e eficácia da desinsfestação de substratos utilizando vapor quente. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Horticultura Brasileira, Brasília, v. 22, n. 2, jul. 2004. Suplemento 2. |
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Idioma: |
Português |
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Notas: |
Trabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 401, jul. 2004. Suplemento 1. |
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Palavras-Chave: |
Substrato. |
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Thesagro: |
Bactéria; Cyperus Rotundus; Desinfestação; Ralstonia Solanacearum; Tiririca; Trevo. |
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Thesaurus NAL: |
Oxalis corniculata. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00874naa a2200241 a 4500
001 1777112
005 2004-11-18
008 2004 bl uuuu u00u1 u #d
100 1 $aSILVA, C. S.
245 $aUniformidade e eficácia da desinsfestação de substratos utilizando vapor quente.
260 $c2004
500 $aTrabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 401, jul. 2004. Suplemento 1.
650 $aOxalis corniculata
650 $aBactéria
650 $aCyperus Rotundus
650 $aDesinfestação
650 $aRalstonia Solanacearum
650 $aTiririca
650 $aTrevo
653 $aSubstrato
700 1 $aNICOLAU PINTO, M.
700 1 $aSILVA, J. B. C. da
773 $tHorticultura Brasileira, Brasília$gv. 22, n. 2, jul. 2004. Suplemento 2.
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