Data/hora: 17/06/2024 03:19:47

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 26/07/2018
Data da última atualização: | 26/07/2018
Autoria: | LIMA, J. T. M.; FONSECA, J. F. da; BALARO, M. F. A.; ESTEVES, L. V.; ASCOLI, F. O.; LEITE, R. C.; RIBEIRO, A. C. S.; DELGADO, K. F.; SOUZA-FABJAN, J. M. G.; TORRES FILHO, R. A.; BRANDÃO, F. Z.
Afiliação: | Peixoto, Renato Mesquita Peixoto, Renato Mesquita2, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; Jeferson Ferreira da Fonseca, CNPC; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.
Título: | Effect of natural mating or laparoscopic artificial insemination in superovulated Santa Inês ewes on superovulatory response, fertility and embryo viability.
Ano de publicação: | 2016
Fonte/Imprenta: | Animal Production Science, v. 56, n. 9, p. 1463-1468, 2016.
Idioma: | Inglês
Conteúdo: | Abstract: This study evaluated the effect of two mating methods (GNM: natural mating or GAI: laparoscopic artificial insemination) on superovulatory response, fertility and embryo yield in superovulated ewes. Fifteen non-pregnant Santa Inês ewes were superovulated and either mated by GNM or GAI in a crossover design. Oestrus was synchronised using intravaginal progestagen sponges for 6 days and on Day 5, 300 IU eCG and 0.0375 mg d-cloprostenol were given. Twelve hours after sponge removal, 0.025 mg gonadotropin-releasing hormone was administered. Superovulation started 48 h after gonadotropin-releasing hormone treatment, using 5 IU/kg follicle-stimulating hormone (pFSH). At the first pFSH dose, new sponges were inserted. At the fifth dose, 0.0375 mg cloprostenol was administered and the sponges were removed. The GNM was mated with rams every 12 h, until the end of oestrus. The ewes of GAI were laparoscopic inseminated with frozen?thawed semen 36 and 48 h after sponge removal. Ultrasonography was performed every 24 h from the beginning of oestrus synchronisation treatment and every 12 h from the second sponge removal to 2 days after the last pFSH dose. Six to seven days after mating, the number of corpora lutea (CL) was evaluated by laparoscopy and the females with >4CL were subjected to embryo collection. The interval from sponge removal to ovulation was shorter (P < 0.05) in the GNM. The overall superovulatory response was 63.3% (19/30), with 60.0% and 66.7% in GNM and GAI, respectively (P > 0.05). The number of recovered structures (6.4 ± 2.4 vs 4.5 ± 3.0), recovery rate (74.0 ± 16.0 vs 52.3 ± 26.5%), number of transferable embryos (3.0 ± 2.9 vs 3.6 ± 2.0) and viability rate (47.2 ± 45.3 vs 77.4 ± 37.1%) did not differ between GAI and GNM (P > 0.05). However, the GAI group showed a higher (P < 0.05) number of unfertilised oocytes (3.1 ± 3.1) and a higher non-fertilisation rate (47.1 ± 45.3%) than the GNM (0.9 ± 2.1 and 11.5 ± 21.5%). The mating method did not affect the superovulatory response, and production of viable embryos although the non-fertilisation rate has been inferior for the AI group.
Palavras-Chave: | Estrus synchronisation; MOET; Oestrus synchronization; Raça Santa Inês.
Thesagro: | Inseminação Artificial; Ovino; Reprodução Animal; Superovulação; Transferência de Embrião.
Thesaurus Nal: | Artificial insemination; Embryo transfer; Sheep; Superovulation.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (37234-1) - UPC - AP - DD -  -  -  - DIS