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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
14/07/1995 |
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Data da última atualização: |
14/07/1995 |
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Autoria: |
FLORES, R. |
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Afiliação: |
Instituto de Agroquimica y Tecnologia de Alimentos (CSIC), Valencia, Spain. |
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Título: |
Detection of citrus exocortis viroid in crude extracts by dot-blot hybridization: Conditions for reducing spurious hybridization results and for enhancing the sensitivity of the technique. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
Journal of Virological methods v.13, p.161-169, 1986. |
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Idioma: |
Espanhol |
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Conteúdo: |
Dot-blot assays to detect citrus exocortis viroid (CEV), in clarified sap and unfractionated total nucleic acid preparations of CEV- infected Gynura aurantiaca and chrysanthemun, were impaired by the non-specific binding of the radioactive probe shown by the healty controls. This non-specific background was considerably reduced by the addition to the hybridization mixture, of the fraction of nucleic from healthy plants which are insoluble in 2 M LiCI (containing mainly the large ribosomal RNAs). Sample denaturation with formaldehyde was found to provide a high increase of hybridization, when compared with samples either denatured with formamide or directly spotted.Nitrocellulose was observed to be a better solid support than charge-modified nylon, in terms of the sensitivity of viroid detection by spot hybridization. |
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Palavras-Chave: |
cDNA; Dot-blot assay; Nucleic acid hybrization. |
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Thesaurus Nal: |
viroids. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01408naa a2200169 a 4500
001 1646675
005 1995-07-14
008 1986 bl --- 0-- u #d
100 1 $aFLORES, R.
245 $aDetection of citrus exocortis viroid in crude extracts by dot-blot hybridization$bConditions for reducing spurious hybridization results and for enhancing the sensitivity of the technique.
260 $c1986
520 $aDot-blot assays to detect citrus exocortis viroid (CEV), in clarified sap and unfractionated total nucleic acid preparations of CEV- infected Gynura aurantiaca and chrysanthemun, were impaired by the non-specific binding of the radioactive probe shown by the healty controls. This non-specific background was considerably reduced by the addition to the hybridization mixture, of the fraction of nucleic from healthy plants which are insoluble in 2 M LiCI (containing mainly the large ribosomal RNAs). Sample denaturation with formaldehyde was found to provide a high increase of hybridization, when compared with samples either denatured with formamide or directly spotted.Nitrocellulose was observed to be a better solid support than charge-modified nylon, in terms of the sensitivity of viroid detection by spot hybridization.
650 $aviroids
653 $acDNA
653 $aDot-blot assay
653 $aNucleic acid hybrization
773 $tJournal of Virological methods$gv.13, p.161-169, 1986.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio-Norte. Para informações adicionais entre em contato com cpamn.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
19/02/1997 |
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Data da última atualização: |
08/01/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PINHEIRO, R. R.; ALVES, F. S. F.; GIRAO, E. S.; MEDEIROS, L. P.; GIRAO, R. N. |
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Título: |
Presenca da artrite encefalite caprina (CAEV) em Teresina, Piaui. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MEDICINA VETERINARIA, 24., 1996, Goiania. Anais.... Goiania, 1996. p. 161. |
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Idioma: |
Português |
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Palavras-Chave: |
Artrite encefalite; Brasil; CAEV; Goat; Piaui. |
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Thesagro: |
Caprino. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00629nam a2200217 a 4500
001 1053464
005 2024-01-08
008 1996 bl uuuu u00u1 u #d
100 1 $aPINHEIRO, R. R.
245 $aPresenca da artrite encefalite caprina (CAEV) em Teresina, Piaui.
260 $aIn: CONGRESSO BRASILEIRO DE MEDICINA VETERINARIA, 24., 1996, Goiania. Anais.... Goiania, 1996. p. 161.$c1996
650 $aCaprino
653 $aArtrite encefalite
653 $aBrasil
653 $aCAEV
653 $aGoat
653 $aPiaui
700 1 $aALVES, F. S. F.
700 1 $aGIRAO, E. S.
700 1 $aMEDEIROS, L. P.
700 1 $aGIRAO, R. N.
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