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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZAFALON, L. F.; SANTANA, R. C. M.; ESTEVES, S. N.; FIM JÚNIOR, G. A. |
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Afiliação: |
LUIZ FRANCISCO ZAFALON, CPPSE; RAUL COSTA MASCARENHAS SANTANA, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; Guilherme Aparecido Fim Júnior, Unesp Jaboticabal. |
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Título: |
Somatic cell count in the diagnosis of subclinical mastitis in sheep of different breeds. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v.39, n.4, p.1555-1564, jul./ago. 2018 |
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ISSN: |
1679-0359 |
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DOI: |
10.5433/1679-0359.2018v39n4p1555 |
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Idioma: |
Inglês |
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Conteúdo: |
The aims of this study were to determine the occurrence of subclinical mastitis in sheep of different breeds and the values for somatic cell count (SCC) in milk for the diagnosis of the disease at lactation and weaning, a fundamental prerequisite for identifying animals in need of control measures. Milk samples were obtained from 1,457 mammary halves of Santa Inês, Texel, Ile de France, and Dorper sheep at two different periods, during the second week of lactation and at weaning. After teats antisepsis, the samples were collected, and identification of the infectious etiology of mastitis and determination of SCC were performed. Microorganisms were identified in 117/762 (15.3%) mammary halves in the second week of lactation and in 86/694 (12.4%) at weaning. Coagulase-negative staphylococci (CoNS) were the etiological agents with the highest incidence alone and in association with other microorganisms, with percentages of 58.1% and 60.6%, respectively. The Santa Inês presented a higher incidence of subclinical mastitis when compared to the other breeds. The cut-off values of SCC for subclinical mastitis were determined at both sampling periods and varied according to stage of lactation, as well breed. These results illustrate the lack of a universal value that can be used for the diagnosis of mastitis and suggests the need for permanent follow-up in herds in order to control the disease. |
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Palavras-Chave: |
Teste diagnóstico. |
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Thesagro: |
Leite; Ovelha. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182166/1/SomaticCellCount.pdf
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Marc: |
LEADER 02084naa a2200217 a 4500
001 2094820
005 2019-05-06
008 2018 bl uuuu u00u1 u #d
022 $a1679-0359
024 7 $a10.5433/1679-0359.2018v39n4p1555$2DOI
100 1 $aZAFALON, L. F.
245 $aSomatic cell count in the diagnosis of subclinical mastitis in sheep of different breeds.$h[electronic resource]
260 $c2018
520 $aThe aims of this study were to determine the occurrence of subclinical mastitis in sheep of different breeds and the values for somatic cell count (SCC) in milk for the diagnosis of the disease at lactation and weaning, a fundamental prerequisite for identifying animals in need of control measures. Milk samples were obtained from 1,457 mammary halves of Santa Inês, Texel, Ile de France, and Dorper sheep at two different periods, during the second week of lactation and at weaning. After teats antisepsis, the samples were collected, and identification of the infectious etiology of mastitis and determination of SCC were performed. Microorganisms were identified in 117/762 (15.3%) mammary halves in the second week of lactation and in 86/694 (12.4%) at weaning. Coagulase-negative staphylococci (CoNS) were the etiological agents with the highest incidence alone and in association with other microorganisms, with percentages of 58.1% and 60.6%, respectively. The Santa Inês presented a higher incidence of subclinical mastitis when compared to the other breeds. The cut-off values of SCC for subclinical mastitis were determined at both sampling periods and varied according to stage of lactation, as well breed. These results illustrate the lack of a universal value that can be used for the diagnosis of mastitis and suggests the need for permanent follow-up in herds in order to control the disease.
650 $aLeite
650 $aOvelha
653 $aTeste diagnóstico
700 1 $aSANTANA, R. C. M.
700 1 $aESTEVES, S. N.
700 1 $aFIM JÚNIOR, G. A.
773 $tSemina: Ciências Agrárias, Londrina$gv.39, n.4, p.1555-1564, jul./ago. 2018
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
12/05/2021 |
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Data da última atualização: |
13/05/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ANDRADE, J. de S.; MOREIRA, E. M.; SILVA, G. M. da; SCHNEIDER, A.; NUNES, V. R. R.; SILVA, R. R. da; PFEIFER, L. F. M. |
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Afiliação: |
JÉSSICA DE SOUZA ANDRADE, Bionorte, Programa de Pós-Graduação em Biodiversidade e Biotecnologia, Porto Velho, RO; ELIZÂNGELA MÍRIAN MOREIRA, Universidade Federal do Amazonas (UFAM); GEORGE MOREIRA DA SILVA, Universidade Federal de Rondônia (UNIR); AUGUSTO SCHNEIDER, Universidade Federal de Pelotas (UFPel); VANESSA RACHELE RIBEIRO NUNES, CPAF-RO; RENATA REIS DA SILVA, CPAF-RO; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO. |
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Título: |
Uterine health and fertility of timed AI postpartum Nelore beef cows raised in the Amazon biome. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Livestock Science, v. 249, 104528, July 2021. |
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ISSN: |
1871-1413 |
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DOI: |
https://doi.org/10.1016/j.livsci.2021.104528 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, transcription of IL-8 was greater (P = 0.02) in SCEP than HE and SCE groups. The relative transcription IL-1B was greater (P < 0.05) in SCEP than in PVD+, but the cows in the HE and SCE groups were not different from the other groups (P = 0.08). Our results demonstrate that postpartum beef cows, raised in the Amazon biome, with a higher proportion of PMN cells have lower fertility. Moreover, ~ 40% of the postpartum cows included in TAI programs early postpartum had suboptimal uterine conditions (subclinical endometritis, purulent vaginal discharge, or both). MenosThis study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, trans... Mostrar Tudo |
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Palavras-Chave: |
Citocina; Cytobrush; Inseminação artifical por tempo determinado; PMN cells; Timed artificial insemination (TAI); Uterine health. |
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Thesagro: |
Fertilidade Animal; Gado de Corte; Gado Nelore; Imunidade; Pós-Parto; Reprodução Animal; Sanidade Animal; Útero; Vaca de Corte. |
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Thesaurus NAL: |
Animal fertility; Animal reproduction; Beef cows; Cytokines; Immunity; Nellore; Postpartum period. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03446naa a2200481 a 4500
001 2131781
005 2021-05-13
008 2021 bl uuuu u00u1 u #d
022 $a1871-1413
024 7 $ahttps://doi.org/10.1016/j.livsci.2021.104528$2DOI
100 1 $aANDRADE, J. de S.
245 $aUterine health and fertility of timed AI postpartum Nelore beef cows raised in the Amazon biome.$h[electronic resource]
260 $c2021
520 $aThis study evaluated the effect of uterine health on the fertility of postpartum beef cows. Multiparous lactating Nelore cows (Bos indicus; n = 155) were subjected to a timed artificial insemination (TAI) protocol. Cows were included in a progesterone-estradiol based TAI protocol. On Day 0, before the beginning of the protocol, cows were subjected to evaluation of purulent vaginal discharge (PVD) and collection of uterine tissue using the cytobrush technique. Slides for polymorphonuclear (PMN) cells counting were prepared before the same cytobrush was used for RNA extraction and analysis of IL-1B, IL-6, IL-8, TNFa, GAPDH, and Bactin gene transcription. Cows were separated into 4 categories according to the proportion of PMN and PVD: 1) Healthy (HE; n = 87), cows with <- 4.75% PMN and PVD of 0; 2) Positive Purulent Vaginal Discharge (PVD+; n = 24), cows with < - 4.75% PMN and PVD >- 1; 3) Subclinical Endometritis (SCE; n = 29), cows with > 4.75% PMN and PVD of 0, and; 4) Subclinical Endometritis + PVD+ (SCEP; n = 15), cows with > 4.75% PMN and PVD ? 1. Cows in the HE group had a greater (P < 0.05) pregnancy per AI (P/IA) than cows in SCE and SCEP groups. However, P/AI in cows from the PVD+ group was not different in comparison to HE and SCE groups (P > 0.05). Cows from the SCE and SCEP groups had a higher (P < 0.001) proportion of PMN cells in the uterus than HE and PVD+ groups. Relative transcription of IL-6 and TNFa did not change among groups (P > 0.05). In contrast, transcription of IL-8 was greater (P = 0.02) in SCEP than HE and SCE groups. The relative transcription IL-1B was greater (P < 0.05) in SCEP than in PVD+, but the cows in the HE and SCE groups were not different from the other groups (P = 0.08). Our results demonstrate that postpartum beef cows, raised in the Amazon biome, with a higher proportion of PMN cells have lower fertility. Moreover, ~ 40% of the postpartum cows included in TAI programs early postpartum had suboptimal uterine conditions (subclinical endometritis, purulent vaginal discharge, or both).
650 $aAnimal fertility
650 $aAnimal reproduction
650 $aBeef cows
650 $aCytokines
650 $aImmunity
650 $aNellore
650 $aPostpartum period
650 $aFertilidade Animal
650 $aGado de Corte
650 $aGado Nelore
650 $aImunidade
650 $aPós-Parto
650 $aReprodução Animal
650 $aSanidade Animal
650 $aÚtero
650 $aVaca de Corte
653 $aCitocina
653 $aCytobrush
653 $aInseminação artifical por tempo determinado
653 $aPMN cells
653 $aTimed artificial insemination (TAI)
653 $aUterine health
700 1 $aMOREIRA, E. M.
700 1 $aSILVA, G. M. da
700 1 $aSCHNEIDER, A.
700 1 $aNUNES, V. R. R.
700 1 $aSILVA, R. R. da
700 1 $aPFEIFER, L. F. M.
773 $tLivestock Science$gv. 249, 104528, July 2021.
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Embrapa Rondônia (CPAF-RO) |
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