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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
22/03/2007 |
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Data da última atualização: |
25/05/2018 |
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Autoria: |
KARAM, D.; WESTRA, P.; NIESSEN, S. J.; WARD, S. M.; FIGUEIREDO, J. E. F. |
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Afiliação: |
DECIO KARAM, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
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Título: |
Assessment of silver-stained AFLP markers for studying DNA polymorphism in proso millet (Panicum miliaceum L.). |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Revista Brasileira de Botânica, São Paulo, v. 29, n. 4, p. 609-615, out./dez. 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
Proso millet (Panicum miliaceum) is a serious weed in North America. A high number of wild proso millet biotypes are known but the genetic basis of its phenotypic variation is poorly understood. In the present study, a non-radioactive silver staining method for PCR Amplified Fragment Length Polymorphism (AFLP) was evaluated for studying genetic polymorphism in American proso millet biotypes. Twelve biotypes and 8 primer combinations with two/three and three/three selective nucleotides were used. Pair of primers with two/three selective nucleotides produced the highest number of amplified DNA fragments, while pair of primers with three/three selective nucleotides were more effective for revealing more polymorphic DNA fragments. The two better primer combinations were EcoR-AAC/Mse-CTT and EcoR-ACT/Mse-CAA with 7 and 11 polymorphic DNA fragments, respectively. In a total of 450 amplified fragments, at least 339 appeared well separated in a silver stained acrylamide gel and 39 polymorphic DNA bands were scored. The level of polymorphic DNA (11.5%) using only 8 pairs of primers were effective for grouping proso millet biotypes in two clusters but insufficient for separating hybrid biotypes from wild and crop. Nevertheless, the present result indicates that silver stained AFLP markers could be a cheap and important tool for studying genetic relationships in proso millet.. |
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Palavras-Chave: |
Diversidade genética. |
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Thesagro: |
Marcador Molecular. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/50807/1/Assessment-silver.pdf
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Marc: |
LEADER 02023naa a2200193 a 4500
001 1490478
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008 2006 bl uuuu u00u1 u #d
100 1 $aKARAM, D.
245 $aAssessment of silver-stained AFLP markers for studying DNA polymorphism in proso millet (Panicum miliaceum L.).$h[electronic resource]
260 $c2006
520 $aProso millet (Panicum miliaceum) is a serious weed in North America. A high number of wild proso millet biotypes are known but the genetic basis of its phenotypic variation is poorly understood. In the present study, a non-radioactive silver staining method for PCR Amplified Fragment Length Polymorphism (AFLP) was evaluated for studying genetic polymorphism in American proso millet biotypes. Twelve biotypes and 8 primer combinations with two/three and three/three selective nucleotides were used. Pair of primers with two/three selective nucleotides produced the highest number of amplified DNA fragments, while pair of primers with three/three selective nucleotides were more effective for revealing more polymorphic DNA fragments. The two better primer combinations were EcoR-AAC/Mse-CTT and EcoR-ACT/Mse-CAA with 7 and 11 polymorphic DNA fragments, respectively. In a total of 450 amplified fragments, at least 339 appeared well separated in a silver stained acrylamide gel and 39 polymorphic DNA bands were scored. The level of polymorphic DNA (11.5%) using only 8 pairs of primers were effective for grouping proso millet biotypes in two clusters but insufficient for separating hybrid biotypes from wild and crop. Nevertheless, the present result indicates that silver stained AFLP markers could be a cheap and important tool for studying genetic relationships in proso millet..
650 $aMarcador Molecular
653 $aDiversidade genética
700 1 $aWESTRA, P.
700 1 $aNIESSEN, S. J.
700 1 $aWARD, S. M.
700 1 $aFIGUEIREDO, J. E. F.
773 $tRevista Brasileira de Botânica, São Paulo$gv. 29, n. 4, p. 609-615, out./dez. 2006.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 158 | |
| 29. |  | KARAM, D.; WESTRA, P.; NIESSEN, S. J.; WARD, S. M.; FIGUEIREDO, J. E. F. Assessment of silver-stained AFLP markers for studying DNA polymorphism in proso millet (Panicum miliaceum L.). Revista Brasileira de Botânica, São Paulo, v. 29, n. 4, p. 609-615, out./dez. 2006.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 39. | | KARAM, D.; WESTRA, P.; NISSEN, S. J.; WARD, S. M.; FIGUEIREDO, J. E. F. Genetic diversity among proso millet (Panicum miliaceum) biotypes assessed by AFLP technique. Planta Daninha, Viçosa, v. 22, n. 2, p. 167-174, 2004.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 158 | |
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