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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
19/07/2021 |
Data da última atualização: |
19/07/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VASCONCELOS, M. J. V. de; SCHAFFERT, R. E.; OLIVEIRA, M. F. de; JAIN, A.; FIGUEIREDO, J. E. F.; RAGHOTHAMA, K. G. |
Afiliação: |
MARIA JOSE VILACA DE VASCONCELOS, CNPMS; ROBERT EUGENE SCHAFFERT, CNPMS; MAURILIO FERNANDES DE OLIVEIRA, CNPMS; A. JAIN, National Research Centre on Plant Biotechnology; JOSE EDSON FONTES FIGUEIREDO, CNPMS; K.G. RAGHOTHAMA, Purdue University. |
Título: |
Isolation of high-affinity phosphate transporters SbPT1 AND SbPT2 in Sorghum bicolor and their characterization in contrasting genotypes. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 20, n. 2, gmr18717, 2021. |
DOI: |
http://dx.doi.org/10.4238/gmr18717 |
Idioma: |
Inglês |
Conteúdo: |
Phosphate (Pi) availability is highly limited in the acidic soils of the Brazilian savannahs (Cerrado) used for sorghum cultivation. Although several sorghum genotypes contrasting for P use-efficiency have been developed from natural genetic variants, the Pi transport pathway mechanisms in these plants remain unclear. High-affinity Pi transporters play a pivotal role in Pi acquisition by roots and its subsequent mobilization to aerial parts of the plant. We investigated the potential roles of high-affinity Pi transporters in Pi use efficiency in contrasting genotypes of sorghum. A cDNA library prepared from Pi-deprived sorghum seedlings was screened with heterologous Zea mays (maize) Pi transporters ZmPTs, leading to isolation of two homologous sorghum genes referred to as SbPT 1 and SbPT2. Southern analysis revealed that a small gene family represents the SbPTs genes in the sorghum genome. There were significant increases in the transcription levels of SbPT1 and SbPT2 in roots of Pi-deprived seedlings of both Pi-use efficient (101B) and Pi-use inefficient (136B) genotypes. A decrease in the transcript levels of these transporters in 101B and 136B upon Pi replenishment suggested their transcriptional regulation by Pi. Although SbPT1 and SbPT2 were induced in the roots, and in young and old leaves of Pi-deprived sorghum, high transcription levels were observed exclusively in the stems of Pi-efficient genotype 101B under Pi-deprivation. This suggests a role of SbPTs in the efficient mobilization of Pi from the root to the shoot, which could be one of the factors conferring higher Pi-use efficiency in this genotype. MenosPhosphate (Pi) availability is highly limited in the acidic soils of the Brazilian savannahs (Cerrado) used for sorghum cultivation. Although several sorghum genotypes contrasting for P use-efficiency have been developed from natural genetic variants, the Pi transport pathway mechanisms in these plants remain unclear. High-affinity Pi transporters play a pivotal role in Pi acquisition by roots and its subsequent mobilization to aerial parts of the plant. We investigated the potential roles of high-affinity Pi transporters in Pi use efficiency in contrasting genotypes of sorghum. A cDNA library prepared from Pi-deprived sorghum seedlings was screened with heterologous Zea mays (maize) Pi transporters ZmPTs, leading to isolation of two homologous sorghum genes referred to as SbPT 1 and SbPT2. Southern analysis revealed that a small gene family represents the SbPTs genes in the sorghum genome. There were significant increases in the transcription levels of SbPT1 and SbPT2 in roots of Pi-deprived seedlings of both Pi-use efficient (101B) and Pi-use inefficient (136B) genotypes. A decrease in the transcript levels of these transporters in 101B and 136B upon Pi replenishment suggested their transcriptional regulation by Pi. Although SbPT1 and SbPT2 were induced in the roots, and in young and old leaves of Pi-deprived sorghum, high transcription levels were observed exclusively in the stems of Pi-efficient genotype 101B under Pi-deprivation. This suggests a role of SbPTs in the eff... Mostrar Tudo |
Thesagro: |
Fosfato; Genótipo; Sorghum Bicolor. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224540/1/Isolation-of-high-affinity.pdf
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Marc: |
LEADER 02398naa a2200229 a 4500 001 2133015 005 2021-07-19 008 2021 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.4238/gmr18717$2DOI 100 1 $aVASCONCELOS, M. J. V. de 245 $aIsolation of high-affinity phosphate transporters SbPT1 AND SbPT2 in Sorghum bicolor and their characterization in contrasting genotypes.$h[electronic resource] 260 $c2021 520 $aPhosphate (Pi) availability is highly limited in the acidic soils of the Brazilian savannahs (Cerrado) used for sorghum cultivation. Although several sorghum genotypes contrasting for P use-efficiency have been developed from natural genetic variants, the Pi transport pathway mechanisms in these plants remain unclear. High-affinity Pi transporters play a pivotal role in Pi acquisition by roots and its subsequent mobilization to aerial parts of the plant. We investigated the potential roles of high-affinity Pi transporters in Pi use efficiency in contrasting genotypes of sorghum. A cDNA library prepared from Pi-deprived sorghum seedlings was screened with heterologous Zea mays (maize) Pi transporters ZmPTs, leading to isolation of two homologous sorghum genes referred to as SbPT 1 and SbPT2. Southern analysis revealed that a small gene family represents the SbPTs genes in the sorghum genome. There were significant increases in the transcription levels of SbPT1 and SbPT2 in roots of Pi-deprived seedlings of both Pi-use efficient (101B) and Pi-use inefficient (136B) genotypes. A decrease in the transcript levels of these transporters in 101B and 136B upon Pi replenishment suggested their transcriptional regulation by Pi. Although SbPT1 and SbPT2 were induced in the roots, and in young and old leaves of Pi-deprived sorghum, high transcription levels were observed exclusively in the stems of Pi-efficient genotype 101B under Pi-deprivation. This suggests a role of SbPTs in the efficient mobilization of Pi from the root to the shoot, which could be one of the factors conferring higher Pi-use efficiency in this genotype. 650 $aFosfato 650 $aGenótipo 650 $aSorghum Bicolor 700 1 $aSCHAFFERT, R. E. 700 1 $aOLIVEIRA, M. F. de 700 1 $aJAIN, A. 700 1 $aFIGUEIREDO, J. E. F. 700 1 $aRAGHOTHAMA, K. G. 773 $tGenetics and Molecular Research$gv. 20, n. 2, gmr18717, 2021.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
03/12/2012 |
Data da última atualização: |
25/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
VIEIRA, L. de J.; SOARES, T. L.; ROSSI, M. L.; ALVES, A. A. C.; SANTOS, F. de A. R. dos; SOUZA, F. V. D. |
Afiliação: |
LÍVIA DE JESUS VIEIRA, UFRB; TALIANE LEILA SOARES, UFRB; MÔNICA LANZONI ROSSI, USP; ALFREDO AUGUSTO CUNHA ALVES, SRI; FRANCISCO DE ASSIS RIBEIRO DOS SANTOS, UEFS; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
Título: |
Viability, production and morphology of pollen grains for diff erent species in the genus Manihot (Euphorbiaceae). |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Acta Botanica Brasilica, v. 26, n. 2, p. 350-356, 2012. |
ISSN: |
0102-3306 |
Idioma: |
Inglês |
Conteúdo: |
(Viability, production and morphology of pollen grains for different species in the genus Manihot (Euphorbiaceae)). Th e objective of this work was to characterize the viability, production and morphology of pollen for different species in the genus Manihot. Floral buds from Manihot accessions were collected from two germplasm banks at Embrapa Cassava & Fruits. The viability of the pollen was assessed via colorimetric, in vitro and in vivo assays. The diameter of the pollen grains was determined by measuring the transversal length of the grain. The experimental design was entirely randomized. Studies on pollen ultrastructure were performed via scanning electron microscopy. Pollen viability was high in the colorimetric tests and intermediate in vivo tests; there was no germination in the in vitro tests. The average production for all accessions was 1,253 pollen grains per fl oral bud. The size of the pollen grains varied from 132 to 163 µm in the wild accessions, and 129 to 146 µm in the cultivated accessions. Th e pollen grains for all accessions were very large, apolar, spherical as well as inaperturate, with an exine ornamented with pila organized in a Croton pattern. Th e wild accessions, in general, produced more and larger pollen grains compared with the cultivated accessions. |
Palavras-Chave: |
Melhoramento genético. |
Thesagro: |
Mandioca; Manihot Esculenta. |
Thesaurus NAL: |
Breeding and Genetic Improvement; Genetic resources. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02093naa a2200253 a 4500 001 1940940 005 2023-05-25 008 2012 bl uuuu u00u1 u #d 022 $a0102-3306 100 1 $aVIEIRA, L. de J. 245 $aViability, production and morphology of pollen grains for diff erent species in the genus Manihot (Euphorbiaceae).$h[electronic resource] 260 $c2012 520 $a(Viability, production and morphology of pollen grains for different species in the genus Manihot (Euphorbiaceae)). Th e objective of this work was to characterize the viability, production and morphology of pollen for different species in the genus Manihot. Floral buds from Manihot accessions were collected from two germplasm banks at Embrapa Cassava & Fruits. The viability of the pollen was assessed via colorimetric, in vitro and in vivo assays. The diameter of the pollen grains was determined by measuring the transversal length of the grain. The experimental design was entirely randomized. Studies on pollen ultrastructure were performed via scanning electron microscopy. Pollen viability was high in the colorimetric tests and intermediate in vivo tests; there was no germination in the in vitro tests. The average production for all accessions was 1,253 pollen grains per fl oral bud. The size of the pollen grains varied from 132 to 163 µm in the wild accessions, and 129 to 146 µm in the cultivated accessions. Th e pollen grains for all accessions were very large, apolar, spherical as well as inaperturate, with an exine ornamented with pila organized in a Croton pattern. Th e wild accessions, in general, produced more and larger pollen grains compared with the cultivated accessions. 650 $aBreeding and Genetic Improvement 650 $aGenetic resources 650 $aMandioca 650 $aManihot Esculenta 653 $aMelhoramento genético 700 1 $aSOARES, T. L. 700 1 $aROSSI, M. L. 700 1 $aALVES, A. A. C. 700 1 $aSANTOS, F. de A. R. dos 700 1 $aSOUZA, F. V. D. 773 $tActa Botanica Brasilica$gv. 26, n. 2, p. 350-356, 2012.
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