| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
|
Data corrente: |
14/12/2020 |
|
Data da última atualização: |
14/12/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FIGUEIRO, M. R.; GARCIA, J. M.; LIMA, M. R. de; COLLADO, M. del; SARAIVA, N. Z. |
|
Afiliação: |
MARIVALDO RODRIGUES FIGUEIRO, CPATU; Joaquim Mansano Garcia, UNESP; Marina Ragagnin de Lima, UNESP; Maite del Collado, USP; NAIARA ZOCCAL SARAIVA, CNPGL. |
|
Título: |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes? |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Zygote, v. 28, n. 2, p. 109-115, Apr. 2020. |
|
DOI: |
https://doi.org/10.1017/S096719941900073X |
|
Idioma: |
Inglês |
|
Conteúdo: |
In vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production. MenosIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 ... Mostrar Tudo |
|
Palavras-Chave: |
Desenvolvimento embrionário; Soro fetal bovino. |
|
Thesagro: |
Búfalo; Lipídio. |
|
Thesaurus Nal: |
Buffaloes; Lipid metabolism. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02374naa a2200253 a 4500
001 2128094
005 2020-12-14
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S096719941900073X$2DOI
100 1 $aFIGUEIRO, M. R.
245 $aIs it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes?$h[electronic resource]
260 $c2020
520 $aIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production.
650 $aBuffaloes
650 $aLipid metabolism
650 $aBúfalo
650 $aLipídio
653 $aDesenvolvimento embrionário
653 $aSoro fetal bovino
700 1 $aGARCIA, J. M.
700 1 $aLIMA, M. R. de
700 1 $aCOLLADO, M. del
700 1 $aSARAIVA, N. Z.
773 $tZygote$gv. 28, n. 2, p. 109-115, Apr. 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Amazônia Oriental (CPATU) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 57 | |
| 7. |  | ALMEIDA, E. N. de; FIGUEIRO, M. R.; SARAIVA, N. Z.; MORAES, A. J. G. de. Avaliação de adoção de boas práticas para melhoramento genético da pecuária bubalina no município de Cachoeira do Arari, Ilha do Marajó, estado do Pará. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 58., 2020, Foz do Iguaçu. Cooperativismo, inovação e sustentabilidade para o desenvolvimento rural: anais... Foz do Iguaçu: UNIOESTE, 2020. 58º SOBER.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|    |
| 11. | | TAPPEMBECK, M. de F. C.; FIGUEIRÓ, M. R.; SARAIVA, N. Z.; MARQUES, J. R. F. Núcleo de conservação de recursos genéticos animais da Amazônia Oriental (BAGAM). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 18.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 2., 2014, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2014. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 12. | | MARQUES, J. R. F.; FIGUEIRO, M. R.; COSTA, J. S.; MARQUES, L. C.; COSTA, M. R. O mini-cavalo (Equus caballus) puruca da ilha de Marajó - Brasil. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 14. | | BRITO, L. G.; BARBIERI, F. da S.; FIGUEIRO, M. R.; SILVA, W. C. da. Perfil da pecuária leiteira em Rondônia. In: CONGRESSO INTERNACIONAL DO LEITE, 12.; WORKSHOP DE POLÍTICAS PÚBLICAS, 12.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 13., 2013, Porto Velho. Anais... Brasília, DF: Embrapa, 2013. 4p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Rondônia. |
| |
| 16. | | MAROSO, R. D.; BARBIERI, F. da S.; BRITO, L. G.; FIGUEIRO, M. R.; BANDEIRA, P. F.; LANZONI, M. M. Identificação das espécies do gênero Eimeria em bezerros bovinos e bubalinos criados em Rondônia. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande. [Anais...] Campo Grande: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
| |
| 17. | | COSTA, J. da S.; FIGUEIRÓ, M. R.; MARQUES, L. C.; SALES, R. L.; SCHIERHOLT, A. S.; MARQUES, J. R. F. Comportamento produtivo de muçuãs (Kinosternon scorpioides spp. Linnaeus, 1766) na ilha de Marajó, estado do Pará. Amazônia: Ciência e Desenvolvimento, Belém, PA, v. 11, n. 21, jul./dez. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 18. | | MATOS, A. S.; FIGUEIRO, M. R.; BRITO, L. G.; MARCONDES, C. R.; MARQUES, J. R. F.; PAZ, C. C. da. Desenvolvimento ponderal e correlação com produção de leite em rebanho de búfalas sob controle leiteiro no estado de Rondônia. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 46., 2009, Maringá. Inovação científica e tecnológica em zootecnia: anais dos resumos. Maringa: SBZ: UEM, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
| |
| 20. | | GAMA, M. de M. B.; ROCHA, R. B.; SALMAN, A. K. D.; MENDES, A. M.; FIGUEIRO, M. R. Reforestation feasibility in area formerly used for cattle rasing in the state of Rondonia, Northwest Brasilian Amazon. Revista Árvore, Viçosa-MG, v.37, n.6, p.1001-1010, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Rondônia. |
| |
| Registros recuperados : 57 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
