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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
15/08/2016 |
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Data da última atualização: |
15/08/2016 |
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Autoria: |
SPANAMBERG, A.; FERREIRO, L.; MACHADO, G.; FRAGA, C. F.; ARAUJO, R. |
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Afiliação: |
ANDRÉIA SPANAMBERG, PPGCV/UFRGS; LAERTE FERREIRO, UFRGS; GUSTAVO MACHADO, PPGCV/UFRGS; CIBELE FLORIANO FRAGA, PPGCV/UFRGS; RICARDO ARAUJO, IPATIMUP/Universidade do Porto. |
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Título: |
Identification and characterization of Aspergillus fumigatus isolates from broilers. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v.36, n. 7, p. 591-594, jul. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Aspergillosis is one of the main causes of mortality in birds. The pulmonary system is most frequently affected, with lesions observed in the air sacs and lungs of a wide variety of bird species. The aim of this study was to confirm by molecular methods the identification and the genetic diversity of Aspergillus fumigatus isolates of lung?s samples from healthy broilers (Galus galus domesticus). Forty-four (9.5%) isolates of lung?s samples were confirmed as A. fumigatus by polymerase chain reaction (PCR) multiplex (amplification of ?-tub and rodA gene fragments). Microsatellite typing for A. fumigatus was used to analyse all avian isolates. Among them, 40 genotypes (90.9%) were observed only one time. The results showed a high variability and multiple genotypes of de A. fumigatus collected from lung?s samples of broilers. |
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Palavras-Chave: |
Genotipagem; Identificação molecular; Molecular identification; Multiplex PCR; PCR multiplex. |
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Thesagro: |
Aspergillus Fumigatus; Frango de corte. |
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Thesaurus Nal: |
Broiler chickens; Genotyping. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/146359/1/Identification-and-characterization.pdf
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Marc: |
LEADER 01639naa a2200277 a 4500
001 2050857
005 2016-08-15
008 2016 bl uuuu u00u1 u #d
100 1 $aSPANAMBERG, A.
245 $aIdentification and characterization of Aspergillus fumigatus isolates from broilers.
260 $c2016
520 $aAspergillosis is one of the main causes of mortality in birds. The pulmonary system is most frequently affected, with lesions observed in the air sacs and lungs of a wide variety of bird species. The aim of this study was to confirm by molecular methods the identification and the genetic diversity of Aspergillus fumigatus isolates of lung?s samples from healthy broilers (Galus galus domesticus). Forty-four (9.5%) isolates of lung?s samples were confirmed as A. fumigatus by polymerase chain reaction (PCR) multiplex (amplification of ?-tub and rodA gene fragments). Microsatellite typing for A. fumigatus was used to analyse all avian isolates. Among them, 40 genotypes (90.9%) were observed only one time. The results showed a high variability and multiple genotypes of de A. fumigatus collected from lung?s samples of broilers.
650 $aBroiler chickens
650 $aGenotyping
650 $aAspergillus Fumigatus
650 $aFrango de corte
653 $aGenotipagem
653 $aIdentificação molecular
653 $aMolecular identification
653 $aMultiplex PCR
653 $aPCR multiplex
700 1 $aFERREIRO, L.
700 1 $aMACHADO, G.
700 1 $aFRAGA, C. F.
700 1 $aARAUJO, R.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv.36, n. 7, p. 591-594, jul. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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