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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
30/01/2017 |
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Data da última atualização: |
02/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, R. C. U.; CANÇADO, L. J.; VALLE, C. B. do; CHIARI, L.; SOUZA, A. P. de. |
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Afiliação: |
REBECCA, C. U. FERREIRA, UNICAMP; LETÍCIA J. CANÇADO; CACILDA BORGES DO VALLE, CNPGC; LUCIMARA CHIARI, CNPGC; ANETE P DE SOUZA, UNICAMP. |
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Título: |
Microsatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
BMC Research Notes, v. 9, n. 152, p. 1-11, 2016. |
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Idioma: |
Português |
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Conteúdo: |
Background: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence in the allelic pool that U. decumbens is closely related to Urochloa ruziziensis and Urochloa brizantha. The genetic distance values determined using Jaccard?s coefficient ranged from 0.06 to 0.76. Conclusions: The microsatellite markers identified in this study are the first set of molecular markers for U. decumbens species. Their availability will facilitate understanding the genetics of this and other Urochloa species and breeding them, and will be useful for germplasm characterization, linkage mapping and marker-assisted selection. Keywords: Enriched library, Forage, Signalgrass, Simple sequence repeat, Transferability MenosBackground: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence ... Mostrar Tudo |
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Palavras-Chave: |
Enriched library; Transferability. |
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Thesaurus Nal: |
Forage; Microsatellite repeats; Urochloa brizantha. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154227/1/Microsatellite-loci-for-Urochloa.pdf
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Marc: |
LEADER 02829naa a2200229 a 4500
001 2062128
005 2017-03-02
008 2016 bl uuuu u00u1 u #d
100 1 $aFERREIRA, R. C. U.
245 $aMicrosatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species.$h[electronic resource]
260 $c2016
520 $aBackground: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other Urochloa species. Findings: Microsatellite loci were isolated from a previously constructed enriched library from one U. decumbens genotype. Specific primers were designed for one hundred thirteen loci, and ninety-three primer pairs successfully amplified microsatellite regions, yielding an average of 4.93 alleles per locus. The polymorphism information content ( IC) values of these loci ranged from 0.26 to 0.85 (average 0.68), and the associated discriminating power (DP) values ranged from 0.22 to 0.97 (average 0.77). Cross-amplification studies demonstrated the potential transferability of these microsatellites to four other Urochloa species. Structure analysis revealed the existence of three distinct groups, providing evidence in the allelic pool that U. decumbens is closely related to Urochloa ruziziensis and Urochloa brizantha. The genetic distance values determined using Jaccard?s coefficient ranged from 0.06 to 0.76. Conclusions: The microsatellite markers identified in this study are the first set of molecular markers for U. decumbens species. Their availability will facilitate understanding the genetics of this and other Urochloa species and breeding them, and will be useful for germplasm characterization, linkage mapping and marker-assisted selection. Keywords: Enriched library, Forage, Signalgrass, Simple sequence repeat, Transferability
650 $aForage
650 $aMicrosatellite repeats
650 $aUrochloa brizantha
653 $aEnriched library
653 $aTransferability
700 1 $aCANÇADO, L. J.
700 1 $aVALLE, C. B. do
700 1 $aCHIARI, L.
700 1 $aSOUZA, A. P. de
773 $tBMC Research Notes$gv. 9, n. 152, p. 1-11, 2016.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Gado de Leite. |
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Data corrente: |
14/11/2023 |
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Data da última atualização: |
14/11/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SCHNEIDER, S.; CASSOL, A. P.; PITALUGA, C.; DINIZ, F. H.; NEVES, J. A. S. das; SILVA, L. de J. de S. |
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Afiliação: |
SERGIO SCHNEIDER, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; ABEL PERINAZZO CASSOL, UNIVERSIDADE FEDERAL DO MARANHÃO; CHRISTIANE PITALUGA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; FABIO HOMERO DINIZ, CNPGL; JONAS ANDERSON SIMÕES DAS NEVES, UNIVERSIDADE FEDERAL DO PAMPA; LINDOMAR DE JESUS DE SOUSA SILVA, CPAA. |
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Título: |
O acesso dos agricultores familiares aos mercados alimentares: políticas públicas, autonomia e segurança alimentar no Brasil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: CONGRESSO DA SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 61., 2023, Piracicaba. Agropecuária do futuro: tecnologia, sustentabilidade e a segurança alimentar: anais. Piracicaba: ESALQ/USP, 2023. |
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Idioma: |
Português |
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Thesagro: |
Agricultura Familiar; Agricultura Sustentável; Comercio; Mercado; Políticas Públicas; Segurança Alimentar. |
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Categoria do assunto: |
E Economia e Indústria Agrícola |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1158337/1/O-acesso-dos-agricultores-familiares-aos-mercados-alimentares.pdf
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Marc: |
LEADER 00924nam a2200229 a 4500
001 2158337
005 2023-11-14
008 2023 bl uuuu u00u1 u #d
100 1 $aSCHNEIDER, S.
245 $aO acesso dos agricultores familiares aos mercados alimentares$bpolíticas públicas, autonomia e segurança alimentar no Brasil.$h[electronic resource]
260 $aIn: CONGRESSO DA SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 61., 2023, Piracicaba. Agropecuária do futuro: tecnologia, sustentabilidade e a segurança alimentar: anais. Piracicaba: ESALQ/USP$c2023
650 $aAgricultura Familiar
650 $aAgricultura Sustentável
650 $aComercio
650 $aMercado
650 $aPolíticas Públicas
650 $aSegurança Alimentar
700 1 $aCASSOL, A. P.
700 1 $aPITALUGA, C.
700 1 $aDINIZ, F. H.
700 1 $aNEVES, J. A. S. das
700 1 $aSILVA, L. de J. de S.
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