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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
22/04/2019 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROCHA, L. C.; FERREIRA, M. T. M.; CUNHA, I. M. F.; MITTELMANN, A.; TECHIO, V. H. |
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Afiliação: |
Laiane Corsini Rocha, UFLA; Marco Túlio Mendes Ferreira, UFLA; Isabela Martinez Fontes Cunha, UFLA; ANDREA MITTELMANN, CNPGL; Vânia Helena Techio, UFLA. |
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Título: |
45S rDNA sites in meiosis of Lolium multiflorum Lam.: variability, non-homologous associations and lack of fragility. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Protoplasma, v. 256, n. 1, p. 227-235, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract In this study, we evaluated the behavior of 45S ribosomal DNA (rDNA) sites during the meiosis of Lolium multiflorum. The reason to study it in this species is that 45S rDNA sites are usually visualized as gaps in mitotic metaphase chromosomes and were initially denominated fragile sites (FSs). In different species, FSs were related to rearrangements that alter the karyotype and affect the chromosome pairing in meiosis. However, our findings show that the chromosome pairing in L. multiflorum is regular and, as in mitosis, the number of sites is variable. In diakinesis with five sites, one of the bivalents was in hemizygous state while, in diakinesis with seven sites, one of the bivalents had three conspicuous signals, two in syntheny in one of the homologous. Only four cells had gaps in the region of the 45S rDNA. Owing to the lower number of signals observed at the initial stages of meiosis, it is assumed that they are involved both in homologous and non-homologous associations and that they might assist the chromosome pairing. Regarding segregation, only meiocytes with five and six 45S rDNA signals were observed, and they were characterized by the segregation of 2/3 signals in the poles of anaphases I up to metaphases II; 2/2 and 3/3 in anaphases II and telophases II; and also 2/2 and 4/4 in the nuclei of tetrads, unlike the number of 45S signals expected. The numerical nonequivalence of sites among nuclei at later stages of meiosis is explained by the presence of chromosomes with hemizygous sites. MenosAbstract In this study, we evaluated the behavior of 45S ribosomal DNA (rDNA) sites during the meiosis of Lolium multiflorum. The reason to study it in this species is that 45S rDNA sites are usually visualized as gaps in mitotic metaphase chromosomes and were initially denominated fragile sites (FSs). In different species, FSs were related to rearrangements that alter the karyotype and affect the chromosome pairing in meiosis. However, our findings show that the chromosome pairing in L. multiflorum is regular and, as in mitosis, the number of sites is variable. In diakinesis with five sites, one of the bivalents was in hemizygous state while, in diakinesis with seven sites, one of the bivalents had three conspicuous signals, two in syntheny in one of the homologous. Only four cells had gaps in the region of the 45S rDNA. Owing to the lower number of signals observed at the initial stages of meiosis, it is assumed that they are involved both in homologous and non-homologous associations and that they might assist the chromosome pairing. Regarding segregation, only meiocytes with five and six 45S rDNA signals were observed, and they were characterized by the segregation of 2/3 signals in the poles of anaphases I up to metaphases II; 2/2 and 3/3 in anaphases II and telophases II; and also 2/2 and 4/4 in the nuclei of tetrads, unlike the number of 45S signals expected. The numerical nonequivalence of sites among nuclei at later stages of meiosis is explained by the presence of ... Mostrar Tudo |
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Palavras-Chave: |
Fragile sites; Hemizygosis; Syntheny of rDNA genes. |
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Thesaurus Nal: |
Chromosome segregation; Forage grasses. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02235naa a2200229 a 4500
001 2108361
005 2023-01-24
008 2019 bl uuuu u00u1 u #d
100 1 $aROCHA, L. C.
245 $a45S rDNA sites in meiosis of Lolium multiflorum Lam.$bvariability, non-homologous associations and lack of fragility.$h[electronic resource]
260 $c2019
520 $aAbstract In this study, we evaluated the behavior of 45S ribosomal DNA (rDNA) sites during the meiosis of Lolium multiflorum. The reason to study it in this species is that 45S rDNA sites are usually visualized as gaps in mitotic metaphase chromosomes and were initially denominated fragile sites (FSs). In different species, FSs were related to rearrangements that alter the karyotype and affect the chromosome pairing in meiosis. However, our findings show that the chromosome pairing in L. multiflorum is regular and, as in mitosis, the number of sites is variable. In diakinesis with five sites, one of the bivalents was in hemizygous state while, in diakinesis with seven sites, one of the bivalents had three conspicuous signals, two in syntheny in one of the homologous. Only four cells had gaps in the region of the 45S rDNA. Owing to the lower number of signals observed at the initial stages of meiosis, it is assumed that they are involved both in homologous and non-homologous associations and that they might assist the chromosome pairing. Regarding segregation, only meiocytes with five and six 45S rDNA signals were observed, and they were characterized by the segregation of 2/3 signals in the poles of anaphases I up to metaphases II; 2/2 and 3/3 in anaphases II and telophases II; and also 2/2 and 4/4 in the nuclei of tetrads, unlike the number of 45S signals expected. The numerical nonequivalence of sites among nuclei at later stages of meiosis is explained by the presence of chromosomes with hemizygous sites.
650 $aChromosome segregation
650 $aForage grasses
653 $aFragile sites
653 $aHemizygosis
653 $aSyntheny of rDNA genes
700 1 $aFERREIRA, M. T. M.
700 1 $aCUNHA, I. M. F.
700 1 $aMITTELMANN, A.
700 1 $aTECHIO, V. H.
773 $tProtoplasma$gv. 256, n. 1, p. 227-235, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
27/10/2017 |
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Data da última atualização: |
27/10/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
TORATI, L. S.; MIGAUD, H.; DOHERTY, M. K.; SIWY, J.; MULLEN, W.; MESQUITA, P. E. C.; ALBALAT, A. |
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Afiliação: |
LUCAS SIMON TORATI, CNPASA; HERVE MIGAUD, UNIVERSITY OF STIRLING, UK; MARY K. DOHERTY, UNIVERSITY OF THE HIGHLANDS AND ISLANDS, UK; JUSTYNA SIWY, MOSAIQUES DIAGNOSTICS GmbH, Hannover-Germany; WILLIAN MULLEN, UNIVERSITY OF GLASGOW, UK; PEDRO E. C. MESQUITA, DNOCS; AMAYA ALBALAT, UNIVERSITY OF STIRLING, UK. |
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Título: |
Comparative proteome and peptidome analysis of the cephalic fluid secreted by Arapaima gigas (Teleostei: Osteoglossidae) during and outside parental care. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plos One, v. 12, n. 10, e0186692, Oct. 2017. |
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ISSN: |
1932-6203 |
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DOI: |
10.1371/journal.pone.0186692 |
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Idioma: |
Inglês |
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Conteúdo: |
Parental investment in Arapaima gigas includes nest building and guarding, followed by a care provision when a cephalic fluid is released from the parents' head to the offspring. This fluid has presumably important functions for the offspring but so far its composition has not been characterised. In this study the proteome and peptidome of the cephalic secretion was studied in parental and non-parental fish using capillary electrophoresis coupled to mass spectrometry (CE-MS) and GeLC-MS/MS analyses. Multiple comparisons revealed 28 peptides were significantly different between males and parental males (PC-males), 126 between females and parental females (PC-females), 51 between males and females and 9 between PC-males and PC-females. Identification revealed peptides were produced in the inner ear (pcdh15b), eyes (tetraspanin and ppp2r3a), central nervous system (otud4, ribeye a, tjp1b and syn1) among others. A total of 422 proteins were also identified and gene ontology analysis revealed 28 secreted extracellular proteins. From these, 2 hormones (prolactin and stanniocalcin) and 12 proteins associated to immunological processes (serotransferrin, α-1-antitrypsin homolog, apolipoprotein A-I, and others) were identified. This study provides novel biochemical data on the lateral line fluid which will enable future hypotheses-driven experiments to better understand the physiological roles of the lateral line in chemical communication. |
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Palavras-Chave: |
Peptidome. |
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Thesagro: |
Peixe; Pirarucu. |
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Thesaurus NAL: |
Alloparental behavior; Arapaima gigas; Proteome. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165650/1/CNPASA-2017-plos.pdf
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Marc: |
LEADER 02319naa a2200289 a 4500
001 2078313
005 2017-10-27
008 2017 bl uuuu u00u1 u #d
022 $a1932-6203
024 7 $a10.1371/journal.pone.0186692$2DOI
100 1 $aTORATI, L. S.
245 $aComparative proteome and peptidome analysis of the cephalic fluid secreted by Arapaima gigas (Teleostei$bOsteoglossidae) during and outside parental care.$h[electronic resource]
260 $c2017
520 $aParental investment in Arapaima gigas includes nest building and guarding, followed by a care provision when a cephalic fluid is released from the parents' head to the offspring. This fluid has presumably important functions for the offspring but so far its composition has not been characterised. In this study the proteome and peptidome of the cephalic secretion was studied in parental and non-parental fish using capillary electrophoresis coupled to mass spectrometry (CE-MS) and GeLC-MS/MS analyses. Multiple comparisons revealed 28 peptides were significantly different between males and parental males (PC-males), 126 between females and parental females (PC-females), 51 between males and females and 9 between PC-males and PC-females. Identification revealed peptides were produced in the inner ear (pcdh15b), eyes (tetraspanin and ppp2r3a), central nervous system (otud4, ribeye a, tjp1b and syn1) among others. A total of 422 proteins were also identified and gene ontology analysis revealed 28 secreted extracellular proteins. From these, 2 hormones (prolactin and stanniocalcin) and 12 proteins associated to immunological processes (serotransferrin, α-1-antitrypsin homolog, apolipoprotein A-I, and others) were identified. This study provides novel biochemical data on the lateral line fluid which will enable future hypotheses-driven experiments to better understand the physiological roles of the lateral line in chemical communication.
650 $aAlloparental behavior
650 $aArapaima gigas
650 $aProteome
650 $aPeixe
650 $aPirarucu
653 $aPeptidome
700 1 $aMIGAUD, H.
700 1 $aDOHERTY, M. K.
700 1 $aSIWY, J.
700 1 $aMULLEN, W.
700 1 $aMESQUITA, P. E. C.
700 1 $aALBALAT, A.
773 $tPlos One$gv. 12, n. 10, e0186692, Oct. 2017.
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Embrapa Pesca e Aquicultura (CNPASA) |
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