Data/hora: 11/06/2024 09:03:17

Biblioteca(s): 	Embrapa Cerrados.	
Data corrente: 	03/09/1996	
Data da última atualização: 	03/09/1996	
Autoria: 	CARVALHO, L. J. C. B.; CASCARDO, J. C. M.; FERREIRA, M. A.; LOUREIRO, M. E.	
Título: 	Studies on proteins and enzymes related to tuberization and starch biosynthesis in cassava roots.	
Ano de publicação: 	1993	
Fonte/Imprenta: 	In: INTERNATIONAL SCIENTIFIC MEETING CASSAVA BIOTECHNOLOGY NETWORK, 1., 1992, Cartagena de Indias, Colombia. Proceedings. Cali: CIAT, 1993.	
Páginas: 	p.234-238.	
Idioma: 	Inglês	
Conteúdo: 	A method has been developed to isolate intact cassava root amyloplast through protoplast preparation. Protoplasts were obtained after incubating cassava root slices in a medium containing cellulase and pectolyase for more than 10 hours at room temperature. After purification iln a discontinuous ficoll density agradient, the protoplasts were ruptured by forcing the suspension through modified disposable syringe. Amyloplast ilntactness was observed in a fluorescence microscopy and activity of amyloplast marker enzymes before and after rupture of the amyloplast membrane. Starch branching enzyme, ADPG-pyrophosphorylase, and starch symthetase were used as amyloplast marker enzymes. The results of the experiment are discussed in a broad starch biosynthesis and tube-rization project that is undergoing in CENARGEN.	
Palavras-Chave: 	Byosynthesis.	
Thesagro: 	Amido; Biossíntese; Enzima; Mandioca; Manihot Esculenta; Proteína.	
Thesaurus Nal: 	cassava; enzymes; proteins; starch.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Cerrados (CPAC)	
Exemplares: 	CPAC - (13198-1) - ADD - PL - -- - S5267 -  - S5267 - DIS