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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
25/05/2009 |
Data da última atualização: |
15/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. |
Afiliação: |
NAIARA ZOCCAL SARAIVA, UNESP/JABOTICABAL; FELIPE PERECIN, FZEA/USP; SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, FZEA/USP; TATIANA ALMEIDA DRUMOND TETZNER, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
Título: |
Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009. |
DOI: |
10.1089/clo.2008.0044 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine. MenosThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst ... Mostrar Tudo |
Thesaurus Nal: |
oocytes. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02377naa a2200205 a 4500 001 1048984 005 2023-03-15 008 2009 bl uuuu u00u1 u #d 024 7 $a10.1089/clo.2008.0044$2DOI 100 1 $aSARAIVA, N. Z. 245 $aDemecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes.$h[electronic resource] 260 $c2009 520 $aThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine. 650 $aoocytes 700 1 $aPERECIN, F. 700 1 $aMÉO, S. C. 700 1 $aFERREIRA, C. R. 700 1 $aTETZNER, T. A. D. 700 1 $aGARCIA, J. M. 773 $tCloning and Stem Cells$gv. 11, n. 1, p. 141-152, mar. 2009.
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7. | | FERREIRA, C. R.; SCORIZA, R. N.; CORREIA, M. E. F.; ROUWS, J. R. C.; RESENDE, A. S. de. Composição da fauna edáfica em diferentes coberturas vegetais no Rio de Janeiro. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG, 2011.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agrobiologia. |
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8. | | FERREIRA, C. R. P.; MARTINS-DA-SILVA, R. C. V.; MARGALHO, L. F.; XAVIER JUNIOR, S. R.; ILKIU-BORGES, F. Levantamento preliminar das espécies da Tribo Coussareae (Rubiaceae) ocorrentes no Estado do Pará. In: JORNADA DE CIÊNCIAS BIOLÓGICAS, 1., 2008, Belém, PA. Biodescobertas: ciência, ambiente e sociedade. Belém, PA: CEFET-PA: MPEG, 2008.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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10. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; VANTINI, R.; GARCIA, J. M. Efeitos da demecolcina sobre a cinética da maturação nuclear e a migração dos grânulos corticais em oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1276, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007. p. 1276.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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11. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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12. | | MÉO, S. C.; YAMAZAKI, W.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; LEAL, C. L. V.; GARCIA, J. M. Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments. Zygote, v. 15, p. 295-306, nov. 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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13. | | BALTAZAR, L. L. de; SANTOS, A. dos; DURANTE, L. G. Y.; CARNEIRO, T.; FERREIRA, C. R.; CORREA, A. M.; TORRES, F. E.; MELO, C. L. P. de. Avaliação de arquitetura de planta de genótipos de feijoeiro comum cultivado em Aquidauana, MS. In: SEMANA AGRONÔMICA, 6.; ENCONTRO TÉCNICO-CIENTÍFICO, 1., 2009, Aquidauana. [Resumos...]. Aquidauana: UEMS, 2009. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agropecuária Oeste. |
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14. | | LANDIM JR, L. P.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; MIGLINO, M. A.; GARCIA, J. M. Análise da expressao gênica do VEGF (Vascular Endothelial Growth Factor) em placentas bovinas de animais clonados, produzidos in vitro e in vivo, pelo método de RT-PCR semiquantitativo. ARS VETERINARIA, v. 22, n. 3, p. 217-222, 2006.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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15. | | CHIARATTI, M. R.; BRESSAN, F. F.; FERREIRA, C. R.; MONZANI, P. S.; MESQUITA, L. G.; CAETANO, A. R.; SMITH, L. C.; VERCESI, A. E.; MEIRELLES, F. V. Correlação positiva entre a quantidade de DNA mitocondrial no zigoto e seu potencial de desenvolvimento a blastocisto. Acta Scientiae Veterinariae, v. 36, supl. 2, p. S522, 2008.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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16. | | NICIURA, S. C. M.; FERREIRA, C. R.; CHIARATTI, M. R.; MEIRELLES, F. V.; REGITANO, L. C. de A.; ALENCAR, M. M. de; BARBOSA, P. F. Caracterização do genótipo mitocondrial de um rebanho da raça bovina Canchim. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 45., 2008, Lavras. Anais... Lavras: UFLA; SBZ, 2008. 1 CD-ROMTipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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17. | | MÉO, S. C.; FERREIRA, C. R.; CHIARATTI, M. R.; MEIRELLES, F. V.; REGITANO, L. C. de A.; ALENCAR, M. M. de; BARBOSA, P. F. Characterization of mitochondrial genotypes in the foundation herd of the Canchim beef cattle breed. Genetics and Molecular Research, v. 8, n. 1, p. 261-267, 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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18. | | BIASE, W. K. F. S.; CAETANO, A. R.; BIASE, F. H.; MERIGHE, G. K. F.; ACCORSI, M.; STRANIERI, P.; WATANABE, Y. F.; FERREIRA, C. R.; MEIRELLES, F. V. Identificação de SNPs nos genes Bmp15, Bmprii, Fgf8 e Fgf10 em vacas Nelore com histórico de aspiração folicular. In: CONGRESSO BRASILEIRO DE GENÉTICA, 53., 2007, Águas de Lindóia. Resumos... Águas de Lindóia: Sociedade Brasileira de Genética, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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19. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes. Genetics and Molecular Research, v. 8, n. 1, p. 76-85, 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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20. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; BIASE, F. H.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas. Reproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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