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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
25/03/2003 |
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Data da última atualização: |
25/03/2003 |
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Autoria: |
MADRUGA, C. R.; LEAL, C. R. B.; FERREIRA, A. M. T.; ARAÚJO, F. R.; BONATO, A. L. V.; KESSLER, R. H.; SCHENK, M. A. M.; SOARES, C. O. |
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Afiliação: |
Embrapa Gado de Corte (Campo Grande, MS). |
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Título: |
Genetic and antigenic analysis of Babesia bigemina isolates from five geographical regios of Brazil. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 22, n. 4, p. 153-160, out./dez. 2002. |
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Idioma: |
Inglês |
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Notas: |
CNPGC. |
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Conteúdo: |
A molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed antigenic diversity. However, B cell epitopes on rhoptries and infected erythrocytes were conserved among all isolates studied. In this study it was possible to identify variable and conserved antigens, which had already been described as potential immunogens. Considering that an attenuated Babesia clone used as immunogen selected populations capable of evading the immunity induced by this vaccine, it is necessary to evaluate more deeply the cross-protection conferred by genetically more distant Brazilian B. bigemina isolates and make an evaluation of the polymorphism degree of variable antigens such as MSA-1 and MSA-2. MenosA molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surfac... Mostrar Tudo |
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Palavras-Chave: |
Antigenic polymorphism; Brasil; Polimorfismo entigenico. |
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Thesagro: |
Babesia Bigemina; Polimorfismo Genético. |
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Thesaurus Nal: |
genetic polymorphism. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03058naa a2200289 a 4500
001 1325152
005 2003-03-25
008 2002 bl uuuu u00u1 u #d
100 1 $aMADRUGA, C. R.
245 $aGenetic and antigenic analysis of Babesia bigemina isolates from five geographical regios of Brazil.
260 $c2002
500 $aCNPGC.
520 $aA molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed antigenic diversity. However, B cell epitopes on rhoptries and infected erythrocytes were conserved among all isolates studied. In this study it was possible to identify variable and conserved antigens, which had already been described as potential immunogens. Considering that an attenuated Babesia clone used as immunogen selected populations capable of evading the immunity induced by this vaccine, it is necessary to evaluate more deeply the cross-protection conferred by genetically more distant Brazilian B. bigemina isolates and make an evaluation of the polymorphism degree of variable antigens such as MSA-1 and MSA-2.
650 $agenetic polymorphism
650 $aBabesia Bigemina
650 $aPolimorfismo Genético
653 $aAntigenic polymorphism
653 $aBrasil
653 $aPolimorfismo entigenico
700 1 $aLEAL, C. R. B.
700 1 $aFERREIRA, A. M. T.
700 1 $aARAÚJO, F. R.
700 1 $aBONATO, A. L. V.
700 1 $aKESSLER, R. H.
700 1 $aSCHENK, M. A. M.
700 1 $aSOARES, C. O.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 22, n. 4, p. 153-160, out./dez. 2002.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 3 | |
| 2. |  | MADRUGA, C. R.; LEAL, C. R. B.; FERREIRA, A. M. T.; ARAUJO, F. R.; BONATO, A. L. V.; KESSLER, R. H.; SCHENK, M. A. M.; SOARES, C. O. Genetic and antigenic analysis of Babesia bigemina isolates from five geographical regions of Brazil. Pesquisa Veterinária Brasileira, v. 22, n. 4, p. 153-160, out./dez. 2002.| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | MADRUGA, C. R.; LEAL, C. R. B.; FERREIRA, A. M. T.; ARAÚJO, F. R.; BONATO, A. L. V.; KESSLER, R. H.; SCHENK, M. A. M.; SOARES, C. O. Genetic and antigenic analysis of Babesia bigemina isolates from five geographical regios of Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 22, n. 4, p. 153-160, out./dez. 2002. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 3 | |
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