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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
22/02/2011 |
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Data da última atualização: |
10/03/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMIRO, D. A.; ESCOUTE, J.; PETITOT, A.-S.; NICOLE, M.; MALUF, M. P.; FERNANDEZ, D. |
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Afiliação: |
INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT; CIRAD; INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT; INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT; MIRIAN PEREZ MALUF, SAPC; INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT. |
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Título: |
Biphasic haustorial differentiation of coffee rust (Hemileia vastatrix race II) associated with defense responses in resistant and susceptible coffee cultivars. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In.: PLANT PATHOLOGY, v. 58, p. 944-955, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced ?pioneer? haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed ?secondary haustoria? which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. Transcripts of the pathogenesis-related (PR) genes CaPR1b and CaPR10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. In contrast, the CaWRKY1 gene and the CaRLK gene were only induced in the susceptible cultivar. These results indicated that the specific resistance of cv. Tupi was expressed after differentiation of the H. vastatrix secondary haustoria. Analysis showed no evidence of specific recognition of coffee rust at the pioneer haustoria stage, suggesting that haustoria components are not recognized by, or not secreted into, the subsidiary and adjacent cells of the stomata. Additionally, the present study provides new insights into the colonization process of the coffee rust fungus. MenosThe objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced ?pioneer? haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed ?secondary haustoria? which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. Transcripts of the pathogenesis-related (PR) genes CaPR1b and CaPR10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. In contrast, the CaWRKY1 gene and the CaRLK gene were only induced in the susceptible cultivar. These results indicated that the specific resistance of cv. Tupi was expressed after differentiation of the H. vastatrix secondary haustoria. Analysis showed no evidence of specific recognition of coffee rust at the pioneer haustoria stage, suggesting that haustoria components are not recognized by, or not secrete... Mostrar Tudo |
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Palavras-Chave: |
Pathogenesis-related genes; Quantitative real-time PCR. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Reactive oxygen species. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29268/1/Biphasic-haustorial.pdf
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Marc: |
LEADER 02423naa a2200229 a 4500
001 1880368
005 2011-03-10
008 2009 bl uuuu u00u1 u #d
100 1 $aRAMIRO, D. A.
245 $aBiphasic haustorial differentiation of coffee rust (Hemileia vastatrix race II) associated with defense responses in resistant and susceptible coffee cultivars.$h[electronic resource]
260 $c2009
520 $aThe objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced ?pioneer? haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed ?secondary haustoria? which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. Transcripts of the pathogenesis-related (PR) genes CaPR1b and CaPR10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. In contrast, the CaWRKY1 gene and the CaRLK gene were only induced in the susceptible cultivar. These results indicated that the specific resistance of cv. Tupi was expressed after differentiation of the H. vastatrix secondary haustoria. Analysis showed no evidence of specific recognition of coffee rust at the pioneer haustoria stage, suggesting that haustoria components are not recognized by, or not secreted into, the subsidiary and adjacent cells of the stomata. Additionally, the present study provides new insights into the colonization process of the coffee rust fungus.
650 $aReactive oxygen species
650 $aCoffea Arábica
653 $aPathogenesis-related genes
653 $aQuantitative real-time PCR
700 1 $aESCOUTE, J.
700 1 $aPETITOT, A.-S.
700 1 $aNICOLE, M.
700 1 $aMALUF, M. P.
700 1 $aFERNANDEZ, D.
773 $tIn.: PLANT PATHOLOGY$gv. 58, p. 944-955, 2009.
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Registro original: |
Embrapa Café (CNPCa) |
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Biblioteca |
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| Registros recuperados : 36 | |
| 7. |  | SANTOS, A. da S. dos; SANTOS, J. L. dos; FERNANDEZ, D.; CURADO, F. F. Participatory evaluation of varieties of maize landraces in the state of Paraiba, Brazil. In: ANNUAL MEETING OF THE SOCIETY FOR ECONOMIC BOTANY, 51., 2010, Xalapa. Agrobiodiversity: lessons form conservation and local development: proceedings. Xalapa, Veracruz: Society for Economic Botany, 2010. p. 93. 1 resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 8. | | RAMIRO, D. A.; TOMA-BRAGHINI, M.; PETITOT, A. -S.; MALUF, M. P.; FERNANDEZ, D. Use of leaf-disk technique for gene expression analysis of the coffee responses to Hemileia vastatrix Infection. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
|  |
| 9. | | ALBUQUERQUE, E. V. S.; LECOULS, A. C.; PETITOT, A. S.; GROSSI DE SÁ, M. F.; FERNANDEZ, D. Early resistance responses of coffee (coffea arabica) to root-knot nematode (Meloidogyne spp.) infection. In: INTERNATIONAL CONGRESS OF NEMATOLOGY, 5, 2008, Brisbane, Australia. Nematodes: 5ICN: down under. [S.l.]: Australasian Association of Nematologists, 2008. p. 128.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | BARROS, E. V. S. A.; LECOULS, A. C.; OLIVEIRA, P. E. F.; SILVA, F. R. da; CARNEIRO, R. M. D. G.; FERNANDEZ, D.; GROSSI de SÁ, M. F. Construção e análise de uma biblioteca subtrativa de cDNAs de plantas Coffea arabica inoculada e não inoculada com o nematóide da galha Meloidogyne paranaensis. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. A era da genômica: da bioestatística à bioinformática: anais. Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2005. p. 449.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | BARROS, E. V. S. A.; LECOULS, A. C.; OLIVEIRA, P. E. F.; SILVA, F. R.; CARNEIRO, R. M. D. G.; FERNANDEZ, D.; GROSSI-de-SÁ, M. F. Construção e análise de uma biblioteca subtrativa de cDNAs de plantas Coffea arabica inoculada e não inoculada com o nematóide da galha Meloidogyne paranaensis. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 59.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | MATTOS, V. da S.; MULET, K.; CARES, J. E.; GOMES, C. B.; FERNANDEZ, D.; SA, M. F. G. de; CARNEIRO, R. M. D. G.; CASTAGNONE-SERENO, P. Development of Diagnostic SCAR Markers for Meloidogyne graminicola, M. Oryzae, and M. salasi associated with irrigated rice fields in Americas. Plant Disease, v. 103, p. 83-88, Jan. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | VIDAL, L. de A.; ALMEIDA, J. D. de; PINTO, E. R. de C.; VEIGA, A. D.; FERNANDEZ, D.; GUERREIRO FILHO, O.; ALBUQUERQUE, E. V. S. de. Obtenção de RNA de bicho-mineiro (Leucoptera coffeella) para transcritômica e silenciamento gênico. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café, 2019.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | LIMA, J. D.; MAIGRET, B.; FERNANDEZ, D.; DECLOQUEMENT, J.; PINHO, D.; ALBUQUERQUE, E. V. S.; RODRIGUES, M. O.; MARTINS, N. F. Searching in silico novel targets for specific coffee rust disease control. Lecture Notes in Computer Science, 11347 LNBI, p. 109-115, 2020. Including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 17. | | ALBUQUERQUE, E. V. S.; CUNHA, W. G.; BARBOSA, A. E. A. D.; COSTA, P. M.; TEIXEIRA, J. B.; VIANNA, G. R.; CABRAL, G. B.; FERNANDEZ, D. Transgenic coffee fruits from Coffea arabica genetically modified by bombardment. In Vitro Cellular & Developmental Biology: plant, v.45, n.5, p. 532-539, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | MATTOS, V. S.; MULLET, K.; JUVENIL ENRIQUE, C.; GOMES, C. B.; FERNANDEZ, D.; DE SÁ, M. F. G.; CARNEIRO, R. M. D. G.; CASTAGNONE-SERENO, P. Variabilidade intraespecífica de isolados de meloidogyne spp. do arroz e marcadores scar para identificação de m. graminicola, M. oryzae E M. salasi. In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 35, 2018, Bento Gonçalves, RS.; Nematologia: problemas emergentes e estratégias de manejo. Anais, palestras e resumos... Pelotas: Embrapa Clima Temperado: Sociedade Brasileira de Nematologia: Bento Gonçalves, 2018. p. 145-146| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | MATTOS, V. S.; MULLET, K.; JUVENIL ENRIQUE, C.; GOMES, C. B.; FERNANDEZ, D.; DE SÁ, M. F. G.; CARNEIRO, R. M. D. G.; CASTAGNONE-SERENO, P. Variabilidade intraespecífica de isolados de meloidogyne spp. do arroz e marcadores scar para identificação de m. graminicola, M. oryzae E M. salasi. In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 35, 2018, Bento Gonçalves, RS.; Nematologia: problemas emergentes e estratégias de manejo. Anais, palestras e resumos... Pelotas: Embrapa Clima Temperado: Sociedade Brasileira de Nematologia: Bento Gonçalves, 2018. 239 p. p. 145-146| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 20. | | VIDAL, L. de A.; GRYNBERG, P.; PETITOT, A.-S.; MOTA, A. P. Z.; TOGAWA, R. C.; FERNANDEZ, D.; FREIRE, E. V. S. A. Validação de sequências candidatas de silenciamento gênico de Meloidogyne Incógnita. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café, 2019.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 36 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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