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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
18/11/1992 |
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Data da última atualização: |
18/11/1992 |
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Autoria: |
HAMMOND, E. G.; DUVICK, D. N.; FEHR, W. R.; HILDEBRAND, D. F.; LACEFIELD, E. C.; PFEIFFER, T. W. |
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Afiliação: |
Dep. of Food Science and Human Nutrition. |
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Título: |
Rapid screening techniques for lipoxygenases in soybean seeds. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Crop Science, v.32, n.3, p.820-821, 1992. |
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Idioma: |
Inglês |
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Conteúdo: |
Soybean [Glycine max] breeders are developing cultivars that lack one or more lipoxygenase enzymes for use in certain food products, but electrophoresis for screening lipoxygenase phenotypes in a large segregating population is expensive and time-consuming. Rapid procedures were developed to screen seeds for lipoxygenase-1, -2, and - 3, based on the obility of these enzymes at the surface of cut or broken seed to form hydroperoxides when exposed to emulsions containing linoleate or linolenate. The peroxides are detected by a color test based on the oxidation of iodide-starch for lipoxygenase-1 or of ferrous thiocyanate for lipoxygenase-2 and -3. The test for lipoxygenase-3 is valid only in the absence of lipoxygenase-2; all other combinations of lipoxygenases can be tested. For more than a few samples, the new procedures aregenerally more rapid, convenient, and less costly than electrophoresis and do not require special equipment. |
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Palavras-Chave: |
Lipoxigenase; Rapid screening test; Soybean; Teste qualitativo rapido. |
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Thesagro: |
Soja. |
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Thesaurus Nal: |
lipoxygenase. |
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Categoria do assunto: |
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Marc: |
LEADER 01610naa a2200253 a 4500
001 1457701
005 1992-11-18
008 1992 bl --- 0-- u #d
100 1 $aHAMMOND, E. G.
245 $aRapid screening techniques for lipoxygenases in soybean seeds.
260 $c1992
520 $aSoybean [Glycine max] breeders are developing cultivars that lack one or more lipoxygenase enzymes for use in certain food products, but electrophoresis for screening lipoxygenase phenotypes in a large segregating population is expensive and time-consuming. Rapid procedures were developed to screen seeds for lipoxygenase-1, -2, and - 3, based on the obility of these enzymes at the surface of cut or broken seed to form hydroperoxides when exposed to emulsions containing linoleate or linolenate. The peroxides are detected by a color test based on the oxidation of iodide-starch for lipoxygenase-1 or of ferrous thiocyanate for lipoxygenase-2 and -3. The test for lipoxygenase-3 is valid only in the absence of lipoxygenase-2; all other combinations of lipoxygenases can be tested. For more than a few samples, the new procedures aregenerally more rapid, convenient, and less costly than electrophoresis and do not require special equipment.
650 $alipoxygenase
650 $aSoja
653 $aLipoxigenase
653 $aRapid screening test
653 $aSoybean
653 $aTeste qualitativo rapido
700 1 $aDUVICK, D. N.
700 1 $aFEHR, W. R.
700 1 $aHILDEBRAND, D. F.
700 1 $aLACEFIELD, E. C.
700 1 $aPFEIFFER, T. W.
773 $tCrop Science$gv.32, n.3, p.820-821, 1992.
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Embrapa Soja (CNPSO) |
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