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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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Data corrente: |
06/04/2020 |
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Data da última atualização: |
20/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, F. A. de; VIGNA, B. B. Z.; SILVA, C. C. da; FAVERO, A. P.; MATTA, F. de P.; AZEVEDO, A. L. S.; SOUZA, A. P. de. |
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Afiliação: |
Fernanda A. de Oliveira, UNICAMP; BIANCA BACCILI ZANOTTO VIGNA, CPPSE; Carla C. da Silva, UNICAMP; ALESSANDRA PEREIRA FAVERO, CPPSE; FREDERICO DE PINA MATTA, CPPSE; ANA LUISA SOUSA AZEVEDO, CNPGL; Anete P. de Souza, UNICAMP. |
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Título: |
Coexpression and transcriptome analyses identify active apomixis-related genes in Paspalum notatum leaves. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
BMC Genomics, v. 21, n. 78, 2020. |
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Páginas: |
15 p. |
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DOI: |
https://doi.org/10.1186/s12864-020-6518-z |
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Idioma: |
Inglês |
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Conteúdo: |
Background - Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results - We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions - Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in this work represent rich resources for future grass breeding programs. MenosBackground - Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results - We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions - Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in thi... Mostrar Tudo |
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Palavras-Chave: |
Differential expression; Gene coexpression network; RNA sequencing. |
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Thesaurus Nal: |
Apomixis; Paspalum. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212171/1/CoexpressionTranscriptomeAnalyses.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1129868/1/Coexpression-and-transcriptome-analyses-identify-active-apomixis-related-genes-in-Paspalum.pdf
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Marc: |
LEADER 02397naa a2200277 a 4500
001 2121557
005 2020-04-20
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s12864-020-6518-z$2DOI
100 1 $aOLIVEIRA, F. A. de
245 $aCoexpression and transcriptome analyses identify active apomixis-related genes in Paspalum notatum leaves.$h[electronic resource]
260 $c2020
300 $a15 p.
520 $aBackground - Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results - We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions - Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in this work represent rich resources for future grass breeding programs.
650 $aApomixis
650 $aPaspalum
653 $aDifferential expression
653 $aGene coexpression network
653 $aRNA sequencing
700 1 $aVIGNA, B. B. Z.
700 1 $aSILVA, C. C. da
700 1 $aFAVERO, A. P.
700 1 $aMATTA, F. de P.
700 1 $aAZEVEDO, A. L. S.
700 1 $aSOUZA, A. P. de
773 $tBMC Genomics$gv. 21, n. 78, 2020.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 254 | |
| 3. |  | FAVERO, A. P. Pré melhoramento. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | FÁVERO, A. P. Pré-melhoramento de amendoim no Brasil. In: ENCONTRO DA SOCIEDADE BRASILEIRA DE MELHORAMENTO DE PLANTAS REGIONAL DF, 1., 2005, Brasília, DF. [Anais...]. Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 53. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 144).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | FAVERO, A. P.; VALLS, J. F. M. Arachis repens: grama amendoim. In: CORADIN, L.; CAMILLO, J.; PAREYN, F. G. C. (Ed.). Espécies nativas da flora brasileira de valor econômico atual ou potencial: plantas para o futuro região Nordeste. Brasília, DF: Ministério do Meio Ambiente, 2018. 1085-1088 (Biodiversidade 51)| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | FÁVERO, A. P.; GODOY, I. J. de. Pré-melhoramento de amendoim. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia: Fundação de Apoio à Pesquisa Científica e Tecnológica - FUNCREDI, 2008. p.75.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 254 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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