Data/hora: 12/06/2024 08:54:35

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 04/09/2023
Data da última atualização: | 06/09/2023
Autoria: | SILVA, G. H. da; FRANQUI, L. S.; FARIAS, M. A. de; CASTRO, V. L. S. S. de; BYRNE, H. J.; MARTINEZ, D. S.; MONTEIRO, R. T.; CASEY, A.
Afiliação: | GABRIELA H. DA SILVA, CENTRO NACIONAL DE PESQUISA EM ENERGIA E MATERIAIS; LIDIANE SILVA FRANQUI, CENTRO NACIONAL DE PESQUISA EM ENERGIA E MATERIAIS; MARCELO A. DE FARIAS, CENTRO NACIONAL DE PESQUISA EM ENERGIA E MATERIAIS; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; HUGH J. BYRNE, FOCAS RESEARCH INSTITUTE; DIEGO S.T. MARTINEZ, UNIVERSIDADE DE SÃO PAULO; REGINA T.R. MONTEIRO, UNIVERSIDADE DE SÃO PAULO; ALAN CASEY, FOCAS RESEARCH INSTITUTE.
Título: | TiO2-MWCNT nanohybrid: Cytotoxicity, protein corona formation and cellular internalisation in RTG-2 fish cell line
Ano de publicação: | 2023
Fonte/Imprenta: | Aquatic Toxicology, v.257, article106434, 2023.
ISSN: | 0166-445X
DOI: | https://doi.org/10.1016/j.aquatox.2023.106434
Idioma: | Português
Conteúdo: | Abstract: Titanium dioxide nanoparticles-multiwalled carbon nanotubes (TiO2-MWCNT) nanohydrid has an enhanced photocatalytic activity across the visible light with promising applications in environmental remediation, solar energy devices and antimicrobial technologies. However, it is necessary to evaluate the toxicological effects of TiO2-MWCNT towards safe and sustainable development of nanohybrids. In this work, we studied the cytotoxicity, protein corona formation and cellular internalisation of TiO2-MWCNT on fibroblasts derived from gonadal rainbow trout tissue (RTG-2) for the first time. This nanohydrid did not show any toxicity effect on RTG-2 cells up to 100 mg L-1 after 24 h of exposure as monitored by alamar blue, neutral red and trypan blue assays (in presence or absence of foetal bovine serum, FBS). Futhermore, cryo-transmission electron microscopy analysis demonstrated that TiO2 particles is attached on nanotube surface after FBS-protein corona formation in cell culture medium. Raman spectroscopy imaging showed that TiO2-MWCNT can be internalised by RTG-2 cells. This work is a novel contribution towards better understanding the nanobiointeractions of nanohydrids linked to their in vitro effects on fish cells in aquatic nanoecotoxicology.
Palavras-Chave: | Nanosafety; Protein corona.
Thesagro: | Composto de Carbono; Peixe de Água Doce; Toxidez.
Thesaurus Nal: | Carbon nanotubes; Ecotoxicology; Nanoparticles; Raman spectroscopy; Titanium dioxide.
Categoria do assunto: | W Química e Física
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (17447-1) - UPC - AP - DD -  -  -  - DIS