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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
21/08/2002 |
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Data da última atualização: |
21/08/2002 |
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Autoria: |
NEPOMUCENO, A. L.; OOSTERHUIS, D.; STEWART, J. M.; TURLEY, R.; NEUMAIER, N.; FARIAS, J. R. B. |
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Título: |
Expression of heat shock protein and trehalose-6-phosphate synthase homologues induced during water deficit in cotton. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Brazilian Journal of Plant Physiology, Londrina, v. 14, n. 1, p.11-20, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Tolerance to drought in plants is not a simple trait, but a complex of mechanisms working in combination to avoid or to resist water deficit. Genotypes that differ in tolerance to water deficit may show qualitative and quantitative differences in gene expression when submitted to drought periods. Four cotton (Gossypium hirsutum L.) genotypes (Siokra L-23, Stoneville 506, CS 50 and T-1521) with contrasting responses to water deficit stress were studied using the Differential Display (DD) technique to identify and isolate genes which may differ among them. Fifty-two cDNA fragments differentially expressed during water deficit were isolated, cloned and sequenced. Search of gene bank databases showed that two cDNA clones, A12B15-6 and A12B13-1, have high homology with a heat shock protein that binds to calmodulin found in Nicotiana tabacum (2.9e-32 P(N)) and with an Arabidopsis thaliana trehalose-6-phosphate synthase enzyme (9.0e-37 P(N)), respectively. One of the presumed functions of heat shock proteins is related to prevention of protein denaturation during cellular dehydration. Trehalose-6-phosphate synthase is involved in the production of trehalose, a disaccharide known to osmotically protect cell membranes during dehydration. The HSP homologue was found to be differentially expressed during the drought period in two drought tolerant genotypes but not in drought-sensitive genotypes. The trehalose-6-phosphate synthase homologue was also up-regulated during water deficit stress, however, all four genotypes were induced to express this homologue. Ribonuclease protection assays confirmed these results. This is an important finding since there are only few reports of trehalose presence in higher plants and none in cotton. MenosTolerance to drought in plants is not a simple trait, but a complex of mechanisms working in combination to avoid or to resist water deficit. Genotypes that differ in tolerance to water deficit may show qualitative and quantitative differences in gene expression when submitted to drought periods. Four cotton (Gossypium hirsutum L.) genotypes (Siokra L-23, Stoneville 506, CS 50 and T-1521) with contrasting responses to water deficit stress were studied using the Differential Display (DD) technique to identify and isolate genes which may differ among them. Fifty-two cDNA fragments differentially expressed during water deficit were isolated, cloned and sequenced. Search of gene bank databases showed that two cDNA clones, A12B15-6 and A12B13-1, have high homology with a heat shock protein that binds to calmodulin found in Nicotiana tabacum (2.9e-32 P(N)) and with an Arabidopsis thaliana trehalose-6-phosphate synthase enzyme (9.0e-37 P(N)), respectively. One of the presumed functions of heat shock proteins is related to prevention of protein denaturation during cellular dehydration. Trehalose-6-phosphate synthase is involved in the production of trehalose, a disaccharide known to osmotically protect cell membranes during dehydration. The HSP homologue was found to be differentially expressed during the drought period in two drought tolerant genotypes but not in drought-sensitive genotypes. The trehalose-6-phosphate synthase homologue was also up-regulated during water deficit str... Mostrar Tudo |
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Categoria do assunto: |
-- |
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URL: |
https://www.scielo.br/pdf/bjpp/v14n1/11778.pdf
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Marc: |
LEADER 02321naa a2200181 a 4500
001 1444492
005 2002-08-21
008 2002 bl uuuu u00u1 u #d
100 1 $aNEPOMUCENO, A. L.
245 $aExpression of heat shock protein and trehalose-6-phosphate synthase homologues induced during water deficit in cotton.
260 $c2002
520 $aTolerance to drought in plants is not a simple trait, but a complex of mechanisms working in combination to avoid or to resist water deficit. Genotypes that differ in tolerance to water deficit may show qualitative and quantitative differences in gene expression when submitted to drought periods. Four cotton (Gossypium hirsutum L.) genotypes (Siokra L-23, Stoneville 506, CS 50 and T-1521) with contrasting responses to water deficit stress were studied using the Differential Display (DD) technique to identify and isolate genes which may differ among them. Fifty-two cDNA fragments differentially expressed during water deficit were isolated, cloned and sequenced. Search of gene bank databases showed that two cDNA clones, A12B15-6 and A12B13-1, have high homology with a heat shock protein that binds to calmodulin found in Nicotiana tabacum (2.9e-32 P(N)) and with an Arabidopsis thaliana trehalose-6-phosphate synthase enzyme (9.0e-37 P(N)), respectively. One of the presumed functions of heat shock proteins is related to prevention of protein denaturation during cellular dehydration. Trehalose-6-phosphate synthase is involved in the production of trehalose, a disaccharide known to osmotically protect cell membranes during dehydration. The HSP homologue was found to be differentially expressed during the drought period in two drought tolerant genotypes but not in drought-sensitive genotypes. The trehalose-6-phosphate synthase homologue was also up-regulated during water deficit stress, however, all four genotypes were induced to express this homologue. Ribonuclease protection assays confirmed these results. This is an important finding since there are only few reports of trehalose presence in higher plants and none in cotton.
700 1 $aOOSTERHUIS, D.
700 1 $aSTEWART, J. M.
700 1 $aTURLEY, R.
700 1 $aNEUMAIER, N.
700 1 $aFARIAS, J. R. B.
773 $tBrazilian Journal of Plant Physiology, Londrina$gv. 14, n. 1, p.11-20, 2002.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 543 | |
| 141. |  | BORKERT, C. M.; FARIAS, J. R. B.; SFREDO, G. J.; TUTIDA, F.; SPOLADORI, C. L. Resposta da soja à adubação e disponibilidade de potássio em latossolo roxo álico. Pesquisa Agropecuaria Brasileira, Brasília, v. 32, n. 11, p. 1119-1129, nov.1997.| Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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| 142. | | BORKERT, C. M.; FARIAS, J. R. B.; SFREDO, G. J.; TUTIDA, F.; SPOLADORI, C. L. Resposta da soja à adubação e disponibilidade de potássio em latossolo roxo distrófico. Pesquisa Agropecuaria Brasileira, Brasília, v. 32, n. 12, p. 1235-1249, dez.1997.| Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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| 143. | | BORKERT, C. M.; SFREDO, G. J.; FARIAS, J. R. B.; TUTIDA, F.; SPOLADORI, C. L. Resposta da soja à adubação e disponibilidade de potássio em latossolo roxo eutrófico. Pesquisa Agropecuária Brasileira, Brasília, v. 32, n. 10, p. 1009-1022, out. 1997.| Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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| 144. | | FARIAS, J. R. B.; NEPOMUCENO, A. L.; NEUMAIER, N.; TOBITA, S.; ALMEIDA, I. R. de. Restrições da disponibilidade hídrica à obtenção de elevados rendimentos de grãos de soja. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 32-33. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 145. | | FRAISSE, C.; PERONDI, D.; KARREI, M.; FARIAS, J. R. B.; BENITEZ, D. Seguro paramétrico como nova ferramenta para gerenciar o risco climático na cultura da soja. In: CONGRESSO BRASILEIRO DE SOJA, 9., 2022, Foz do iguaçu, PR. Desafios para a produtividade sustentável no Mercosul: resumos. Brasília, DF: Embrapa, 2022. Regina Maria Villas Bôas de Campos Leite, Adeney de Freitas Bueno, editores técnicos. resumo 266. p. 291.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 147. | | RAKOCEVIC, M.; NEUMAIER, N.; OLIVEIRA, G. M. de; NEPOMUCENO, A. L.; FARIAS, J. R. B. Heliotropic responses of soybean cultivars at three phenological stages and under two water regimes. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 7, p. 661-670, jul. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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| 148. | | RAKOCEVIC, M.; NEUMAIER, N.; GOUVEIA, W. M. de; FARIAS, J. R. B.; NEPOMUCENO, A. L. Heliotropismo em soja para simulações de interceptação luminosa e fotossíntese. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. p.121, trab. 208. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 150. | | NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B.; OYA, T.; RODRIGUES, O. Identificação, clonagem e sequenciamento de genes diferencialmente expressos em resposta às variações climáticas em soja (04.2000.331-03). In: HOFFMANN-CAMPO, C.B.; SARAIVA, O.F. (Org.). Resultados de pesquisa da Embrapa Soja - 2000: ecofisiologia e biologia molecular. Londrina: Embrapa Soja, 2001. p. 16-19. (Embrapa Soja. Documentos, 164).| Biblioteca(s): Embrapa Soja. |
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| 154. | | NEPOMUCENO, A. L.; STEWART, J. M.; OOSTERHUIS, D.; TURLEY, R.; NEUMAIER, N.; FARIAS, J. R. B. Isolation of a cotton NADP (H) oxidade homologue induced by drought stress. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 7, p. 1407-16, jul. 2000. Título em português: Isolamento de um cDNA em algodão homólogo a uma NADP(H) oxidase e induzido.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 155. | | NEPOMUCENO, A. L.; STEWART, J. M.; OOSTERHUIS, D.; TURLEY, R.; NEUMAIER, N.; FARIAS, J. R. B. Isolation of a cotton NADP(H) oxidase homologue induced by drought stress. Pesquisa Agropecuaria Brasileira, Brasilia, v.35, n.7, p.1407-1416, jul. 2000.| Biblioteca(s): Embrapa Soja. |
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| 156. | | NEPOMUCENO, A. L.; OOSTERHUIS, D.; STEWART, J. M.; TURLEY, R.; NEUMAIER, N.; FARIAS, J. R. B. Expression of heat shock protein and trehalose-6-phosphate synthase homologues induced during water deficit in cotton. Brazilian Journal of Plant Physiology, Londrina, v. 14, n. 1, p.11-20, 2002.| Biblioteca(s): Embrapa Soja. |
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| 157. | | NEPOMUCENO, A. L.; FARIAS, J. R. B.; NEUMAIER, N.; OYA, T.; CATTELAN, A. J.; DELATTRE, N. Estratégias para amenizar impactos decorrentes das adversidades climáticas. In: HOFFMANN-CAMPO, C. B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2001: ecofisiologia e biologia molecular. Londrina: Embrapa Soja, 2002. p. 23-24. (Embrapa Soja. Documentos, 198).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 543 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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