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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
14/01/2022 |
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Data da última atualização: |
12/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROSSI, G. B.; SERAGLIO, S. K. T.; HONAISER, T. C.; TOALDO, I. M.; COSTA, A. C. de O.; FARIA, J. C. de; ARISI, A. C. M. |
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Afiliação: |
GABRIELA BARBOSA ROSSI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; SILUANA KATIA TISCHER SERAGLIO, UNIVERSIDADE FEDERAL DE SANTA CATARINA; TUANY CAMILA HONAISER, UNIVERSIDADE FEDERAL DE SANTA CATARINA; ISABELA MAIA TOALDO, UNIVERSIDADE FEDERAL DE SANTA CATARINA; ANA CAROLINA DE OLIVEIRA COSTA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JOSIAS CORREA DE FARIA, CNPAF; ANA CAROLINA MAISONNAVE ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
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Título: |
Protein profile and antioxidant capacity of processed seeds from two common bean (Phaseolus vulgaris L.) cultivars. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
International Journal of Food Science and Technology, v. 57, n. 7, p. 3934-3943, 2022. |
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ISSN: |
1306-8555 |
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DOI: |
https://doi.org/10.1111/ijfs.15537 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris L.) seeds are an economical protein source rich in bioactive compounds with antioxidant activity. In this work, we evaluated processed seeds from two common bean cultivars regarding protein profile and antioxidant capacity before and after enzymatic digestion in vitro. We provided protein maps by two-dimensional electrophoresis (2-DE) of seeds germinated during 36 h and 72 h. The principal component analysis (PCA) showed that protein abundance had more variation on germination times than the cultivar ones. Seeds germinated for 36 h showed greater antioxidant capacity compared to germination for 72 h and cooking, before and after enzymatic digestion. In protein isolates, cooking associated with digestion provided better antioxidant capacity. Germination time influences protein expression as well as the antioxidant capacity of common beans. Furthermore, in vitro digestion increases the antioxidant capacity of cooked bean protein isolates. |
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Thesagro: |
Antioxidante; Feijão; Phaseolus Vulgaris; Semente. |
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Thesaurus Nal: |
Antioxidants; Beans; Proteins; Seeds. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01917naa a2200313 a 4500
001 2139101
005 2023-12-12
008 2022 bl uuuu u00u1 u #d
022 $a1306-8555
024 7 $ahttps://doi.org/10.1111/ijfs.15537$2DOI
100 1 $aROSSI, G. B.
245 $aProtein profile and antioxidant capacity of processed seeds from two common bean (Phaseolus vulgaris L.) cultivars.$h[electronic resource]
260 $c2022
520 $aCommon bean (Phaseolus vulgaris L.) seeds are an economical protein source rich in bioactive compounds with antioxidant activity. In this work, we evaluated processed seeds from two common bean cultivars regarding protein profile and antioxidant capacity before and after enzymatic digestion in vitro. We provided protein maps by two-dimensional electrophoresis (2-DE) of seeds germinated during 36 h and 72 h. The principal component analysis (PCA) showed that protein abundance had more variation on germination times than the cultivar ones. Seeds germinated for 36 h showed greater antioxidant capacity compared to germination for 72 h and cooking, before and after enzymatic digestion. In protein isolates, cooking associated with digestion provided better antioxidant capacity. Germination time influences protein expression as well as the antioxidant capacity of common beans. Furthermore, in vitro digestion increases the antioxidant capacity of cooked bean protein isolates.
650 $aAntioxidants
650 $aBeans
650 $aProteins
650 $aSeeds
650 $aAntioxidante
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aSemente
700 1 $aSERAGLIO, S. K. T.
700 1 $aHONAISER, T. C.
700 1 $aTOALDO, I. M.
700 1 $aCOSTA, A. C. de O.
700 1 $aFARIA, J. C. de
700 1 $aARISI, A. C. M.
773 $tInternational Journal of Food Science and Technology$gv. 57, n. 7, p. 3934-3943, 2022.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
05/11/2020 |
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Data da última atualização: |
05/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ROCHA, R. F. B.; BAENA, M. M.; ESTOPA, A. de C.; GERVASIO, I. C.; IBELLI, A. M. G.; GIONBELLI, T. R. S.; GIONBELLI, M. P.; FREITAS, R. T. F. de; MEIRELLES, S. L. C. |
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Afiliação: |
RENATA DE FÁTIMA BRETANHA ROCHA, UFL; MARIELLE MOURA BAENA, UFL; ALINE DE CÁSSIA ESTOPA, UFL; IZALLY CARVALHO GERVÁSIO, ESALQ; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; TATHYANE RAMALHO SANTOS GIONBELLI, UFL; MATEUS PIES GIONBELLI, UFL; RILKE TADEU FONSECA DE FREITAS, UFL; SARAH LAGUNA CONCEIÇÃO MEIRELLES, UFL. |
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Título: |
Differential expression of HSF1 and HSPA6 genes and physiological responses in Angus and Simmental cattle breeds. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Thermal Biology, v. 84, p. 92-98, 2019. |
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DOI: |
https://doi.org/10.1016/j.jtherbio.2019.06.002 |
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Idioma: |
Alemão |
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Conteúdo: |
Abstract: The aim of this study was to identify whether more and less adapted Angus and Simmental cattle differed in physiological responses and expression of the heat shock factor 1 (HSF1) and the heat shock 70 kDa protein 6 (HSPA6), when subjected to heat stress. Thirty bulls (n=15 ANG; n=15 SIM), extremes ?more adapted" and "less adapted" within each breed were selected to the heat tolerance test. They were selected according to an index based on the average of the respiratory rate obtained on two hot summer days from one hundred bulls. Before the heat tolerance test day, animals were taken to a paddock with water, grass and shade until 7 a.m. of the following day for morning measurements. They were kept in the barn without access to water and shade until 1 p.m. for the afternoon measurements. Respiratory rate in the morning (MRR) and afternoon (ARR), hair coat surface temperature in the morning (MST) and afternoon (AST), rectal temperature in the morning (MRT) and afternoon (ART) were measured and blood samples were collected for expression analysis of the HSF1 and HSPA6 genes. The MIXED procedure of SAS was used for all statistical analysis. The more adapted Simmental group had lesser values of MRR (P=0.023) and MRT (P=0.095), but there was no difference within Angus breed. The ARR was greater (P=0.004) in less adapted animals for both breeds. The ART was lower in the Simmental breed (P < 0.001). Less adapted had greater levels of mRNA of HSF1 (P=0.06) and HSPA6 (P=0.09). In conclusion, respiratory rate, rectal temperature and expression of the HSF1 and HSPA6 genes can be indicators of thermotolerance in taurine cattle. Both breeds show physiologically similar responses under heat stress conditions. MenosAbstract: The aim of this study was to identify whether more and less adapted Angus and Simmental cattle differed in physiological responses and expression of the heat shock factor 1 (HSF1) and the heat shock 70 kDa protein 6 (HSPA6), when subjected to heat stress. Thirty bulls (n=15 ANG; n=15 SIM), extremes ?more adapted" and "less adapted" within each breed were selected to the heat tolerance test. They were selected according to an index based on the average of the respiratory rate obtained on two hot summer days from one hundred bulls. Before the heat tolerance test day, animals were taken to a paddock with water, grass and shade until 7 a.m. of the following day for morning measurements. They were kept in the barn without access to water and shade until 1 p.m. for the afternoon measurements. Respiratory rate in the morning (MRR) and afternoon (ARR), hair coat surface temperature in the morning (MST) and afternoon (AST), rectal temperature in the morning (MRT) and afternoon (ART) were measured and blood samples were collected for expression analysis of the HSF1 and HSPA6 genes. The MIXED procedure of SAS was used for all statistical analysis. The more adapted Simmental group had lesser values of MRR (P=0.023) and MRT (P=0.095), but there was no difference within Angus breed. The ARR was greater (P=0.004) in less adapted animals for both breeds. The ART was lower in the Simmental breed (P < 0.001). Less adapted had greater levels of mRNA of HSF1 (P=0.06) and HSPA6 (P=0.09... Mostrar Tudo |
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Palavras-Chave: |
Adaptabilidade; Estresse por calor; Gado Angus; Gado taurino; Heat shock 70 kDa protein 6; Heat shock factor 1; HSF1; HSP; HSPA6. |
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Thesagro: |
Clima Tropical; Gado Simental; Genética Animal; Pecuária; Produção Animal. |
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Thesaurus NAL: |
Angus; Animal genetics; Animal production; Cattle breeds; Heat shock proteins; Livestock; Simmental. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03109naa a2200481 a 4500
001 2126312
005 2020-11-05
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jtherbio.2019.06.002$2DOI
100 1 $aROCHA, R. F. B.
245 $aDifferential expression of HSF1 and HSPA6 genes and physiological responses in Angus and Simmental cattle breeds.$h[electronic resource]
260 $c2019
520 $aAbstract: The aim of this study was to identify whether more and less adapted Angus and Simmental cattle differed in physiological responses and expression of the heat shock factor 1 (HSF1) and the heat shock 70 kDa protein 6 (HSPA6), when subjected to heat stress. Thirty bulls (n=15 ANG; n=15 SIM), extremes ?more adapted" and "less adapted" within each breed were selected to the heat tolerance test. They were selected according to an index based on the average of the respiratory rate obtained on two hot summer days from one hundred bulls. Before the heat tolerance test day, animals were taken to a paddock with water, grass and shade until 7 a.m. of the following day for morning measurements. They were kept in the barn without access to water and shade until 1 p.m. for the afternoon measurements. Respiratory rate in the morning (MRR) and afternoon (ARR), hair coat surface temperature in the morning (MST) and afternoon (AST), rectal temperature in the morning (MRT) and afternoon (ART) were measured and blood samples were collected for expression analysis of the HSF1 and HSPA6 genes. The MIXED procedure of SAS was used for all statistical analysis. The more adapted Simmental group had lesser values of MRR (P=0.023) and MRT (P=0.095), but there was no difference within Angus breed. The ARR was greater (P=0.004) in less adapted animals for both breeds. The ART was lower in the Simmental breed (P < 0.001). Less adapted had greater levels of mRNA of HSF1 (P=0.06) and HSPA6 (P=0.09). In conclusion, respiratory rate, rectal temperature and expression of the HSF1 and HSPA6 genes can be indicators of thermotolerance in taurine cattle. Both breeds show physiologically similar responses under heat stress conditions.
650 $aAngus
650 $aAnimal genetics
650 $aAnimal production
650 $aCattle breeds
650 $aHeat shock proteins
650 $aLivestock
650 $aSimmental
650 $aClima Tropical
650 $aGado Simental
650 $aGenética Animal
650 $aPecuária
650 $aProdução Animal
653 $aAdaptabilidade
653 $aEstresse por calor
653 $aGado Angus
653 $aGado taurino
653 $aHeat shock 70 kDa protein 6
653 $aHeat shock factor 1
653 $aHSF1
653 $aHSP
653 $aHSPA6
700 1 $aBAENA, M. M.
700 1 $aESTOPA, A. de C.
700 1 $aGERVASIO, I. C.
700 1 $aIBELLI, A. M. G.
700 1 $aGIONBELLI, T. R. S.
700 1 $aGIONBELLI, M. P.
700 1 $aFREITAS, R. T. F. de
700 1 $aMEIRELLES, S. L. C.
773 $tJournal of Thermal Biology$gv. 84, p. 92-98, 2019.
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