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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
21/01/2019 |
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Data da última atualização: |
12/02/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALVES-FREITAS, D. M. T.; PINHEIRO-LIMA, B.; FARIA, J. C.; LACORTE, C.; RIBEIRO, S. G.; MELO, F. L. |
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Afiliação: |
DIONE M. T. ALVES-FREITAS; BRUNA PINHEIRO-LIMA; JOSIAS CORREA DE FARIA, CNPAF; CRISTIANO CASTRO LACORTE, Cenargen; SIMONE DA GRACA RIBEIRO, Cenargen; FERNANDO L. MELO, UNB. |
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Título: |
Double-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Viruses, v. 11, n. 1, Jan. 2019. |
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ISSN: |
1999-4915 |
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DOI: |
10.3390/v11010090 |
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Idioma: |
Inglês |
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Conteúdo: |
Using double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15?39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean. |
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Palavras-Chave: |
Rhabdovirus. |
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Thesagro: |
Feijão; Mosaico Dourado; Organismo Transgênico; Phaseolus Vulgaris; Vírus. |
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Thesaurus Nal: |
Bean golden mosaic virus; Beans; Cytorhabdovirus; DsRNA viruses; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191029/1/CNPAF-2019-viruses.pdf
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Marc: |
LEADER 02429naa a2200337 a 4500
001 2104582
005 2020-02-12
008 2019 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $a10.3390/v11010090$2DOI
100 1 $aALVES-FREITAS, D. M. T.
245 $aDouble-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line.$h[electronic resource]
260 $c2019
520 $aUsing double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15?39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean.
650 $aBean golden mosaic virus
650 $aBeans
650 $aCytorhabdovirus
650 $aDsRNA viruses
650 $aTransgenic plants
650 $aFeijão
650 $aMosaico Dourado
650 $aOrganismo Transgênico
650 $aPhaseolus Vulgaris
650 $aVírus
653 $aRhabdovirus
700 1 $aPINHEIRO-LIMA, B.
700 1 $aFARIA, J. C.
700 1 $aLACORTE, C.
700 1 $aRIBEIRO, S. G.
700 1 $aMELO, F. L.
773 $tViruses$gv. 11, n. 1, Jan. 2019.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
14/08/2000 |
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Data da última atualização: |
21/11/2019 |
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Autoria: |
MARTIUS, C.; HOFER, H.; FORSTER, B. |
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Afiliação: |
ZEF; SMNK, University of Gottingen; ECT Oekotoxikologie GmbH; Embrapa Amazonia Ocidental. |
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Título: |
Towards modelling of soil fauna and litter decomposition in agroforestry sites in Amazonia. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
In: SHIFT PROJECT ENV 52 (Manaus-AB). Soil fauna and litter decomposition in primary and secondary forests and a mixed culture system in Amazonia. Manaus: SMNK/Embrapa Amazonia Ocidental, 1999. |
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Páginas: |
p. 285-290. |
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Idioma: |
Inglês |
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Notas: |
Report Final 1996-1999. |
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Conteúdo: |
An overview over the approaches to modelling used to analyse the project data. It allows to detect factors that might be used to classify situation (e.g., extreme microclimatic factors, soil temperature thresholds) critical for the survival (biomass) of the soil fauna. |
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Palavras-Chave: |
Amazonas; Brasil; Floresta tropial umida; Manaus. |
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Thesagro: |
Agrossilvicultura; Cultivo Multiplo; Dióxido de Carbono; Fauna Edáfica; Programa de Computador. |
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Thesaurus NAL: |
agroforestry; carbon dioxide; computer software; multiple cropping; soil fauna; tropical rain forests. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01373naa a2200349 a 4500
001 1669570
005 2019-11-21
008 1999 bl uuuu u00u1 u #d
100 1 $aMARTIUS, C.
245 $aTowards modelling of soil fauna and litter decomposition in agroforestry sites in Amazonia.
260 $c1999
300 $ap. 285-290.
500 $aReport Final 1996-1999.
520 $aAn overview over the approaches to modelling used to analyse the project data. It allows to detect factors that might be used to classify situation (e.g., extreme microclimatic factors, soil temperature thresholds) critical for the survival (biomass) of the soil fauna.
650 $aagroforestry
650 $acarbon dioxide
650 $acomputer software
650 $amultiple cropping
650 $asoil fauna
650 $atropical rain forests
650 $aAgrossilvicultura
650 $aCultivo Multiplo
650 $aDióxido de Carbono
650 $aFauna Edáfica
650 $aPrograma de Computador
653 $aAmazonas
653 $aBrasil
653 $aFloresta tropial umida
653 $aManaus
700 1 $aHOFER, H.
700 1 $aFORSTER, B.
773 $tIn: SHIFT PROJECT ENV 52 (Manaus-AB). Soil fauna and litter decomposition in primary and secondary forests and a mixed culture system in Amazonia. Manaus: SMNK/Embrapa Amazonia Ocidental, 1999.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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