Data/hora: 19/06/2024 07:57:32

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	06/09/2016	
Data da última atualização: 	13/11/2017	
Autoria: 	TREML, D.; VENTURELLI, G. L.; BROD, F. C. A.; FARIA, J. C.; ARISI, A. C. M.	
Afiliação: 	DIANA TREML, UNIVERSIDADE FEDERAL DE SANTA CATARINA; GUSTAVO L. VENTURELLI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; FABIO C. A. BROD, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JOSIAS CORREA DE FARIA, CNPAF; ANA C. M. ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA.	
Título: 	Development of an event-specific hydrolysis probe quantitative real-time polymerase chain reaction assay for Embrapa 5.1 genetically modified common bean (Phaseolus vulgaris).	
Ano de publicação: 	2014	
Fonte/Imprenta: 	Journal of Agricultural and Food Chemistry, Easton, v. 62, n. 49, p. 11994-12000, Dec. 2014.	
DOI: 	10.1021/jf503928m	
Idioma: 	Inglês	
Conteúdo: 	A genetically modified (GM) common bean event, namely Embrapa 5.1, resistant to the bean golden mosaic virus (BGMV), was approved for commercialization in Brazil. Brazilian regulation for genetically modified organism (GMO) labeling requires that any food containing more than 1% GMO be labeled. The event-specific polymerase chain reaction (PCR) method has been the primary trend for GMO identification and quantitation because of its high specificity based on the flanking sequence. This work reports the development of an event-specific assay, named FGM, for Embrapa 5.1 detection and quantitation by use of SYBR Green or hydrolysis probe. The FGM assay specificity was tested for Embrapa 2.3 event (a noncommercial GM common bean also resistant to BGMV), 46 non-GM common bean varieties, and other crop species including maize, GM maize, soybean, and GM soybean. The FGM assay showed high specificity to detect the Embrapa 5.1 event. Standard curves for the FGM assay presented a mean efficiency of 95% and a limit of detection (LOD) of 100 genome copies in the presence of background DNA. The primers and probe developed are suitable for the detection and quantitation of Embrapa 5.1.	
Palavras-Chave: 	GMO; QPCR; SYBR green; TaqMan.	
Thesagro: 	Biotecnologia; Engenharia genética; Feijão; Organismo transgênico; Phaseolus vulgaris.	
Thesaurus Nal: 	Beans.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (34462-1) - UPC - AP - DD - 2014 -  - 2014 - DIS