Data/hora: 20/06/2024 02:33:44

Biblioteca(s): 	Embrapa Agroenergia.	
Data corrente: 	15/09/2021	
Data da última atualização: 	15/09/2021	
Autoria: 	STEINDORFF, A. S.; SERRA, L. A.; FORMIGHIERI, E. F.; FARIA, F. P. de; POÇAS-FONSECA, M. J.; ALMEIDA, J. R. M. de.	
Afiliação: 	ANDREI STECCA STEINDORFF; LUANA ASSIS SERRA, Universidade de Brasília; EDUARDO FERNANDES FORMIGHIERI, CNPAE; FABRÍCIA PAULA DE FARIA, Universidade Federal de Goiás; MARCIO JOSÉ POÇAS-FONSECA, Universidade de Brasília; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE.	
Título: 	Insights into the Lignocellulose-Degrading Enzyme System of Humicola grisea var. thermoidea Based on Genome and Transcriptome Analysis.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Microbiology Spectrum, v. 9, n. 2, e01088-21, 2021.	
Descrição Física: 	PDF: il. color.	
DOI: 	https://doi.org/10.1128/Spectrum .01088-21	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Humicola grisea var. thermoidea is a thermophilic ascomycete and important enzyme producer that has an efficient enzymatic system with a broad spectrum of thermostable carbohydrate-active (CAZy) enzymes. These enzymes can be employed in lignocellulose biomass deconstruction and other industrial applications. In this work, the genome of H. grisea var. thermoidea was sequenced. The acquired sequence reads were assembled into a total length of 28.75 Mbp. Genome features correlate with what was expected for thermophilic Sordariomycetes. The transcriptomic data showed that sugar-cane bagasse significantly upregulated genes related to primary metabolism and polysaccharide deconstruction, especially hydrolases, at both pH 5 and pH 8. However, a number of exclusive and shared genes between the pH values were found, especially at pH 8. H. grisea expresses an average of 211 CAZy enzymes (CAZymes), which are capable of acting in different substrates. The top upregulated genes at both pH values represent CAZyme-encoding genes from different classes, including acetylxylan esterase, endo-1,4-b-mannosidase, exoglucanase, and endoglucanase genes. For the first time, the arsenal that the thermophilic fungus H. grisea var. thermoidea possesses to degrade the lignocellulosic biomass is shown. Carbon source and pH are of pivotal importance in regulating gene expression in this organism, and alkaline pH is a key regulatory factor for sugarcane bagasse hydrolysis. This work paves the way for the genetic manipulation and robust biotechnological applications of this fungus.	
Palavras-Chave: 	CAZy enzymes; Genome sequencing; Humicola grisea; PH regulation.	
Thesagro: 	Bagaço; Cana de Açúcar; Variação Genética.	
Thesaurus Nal: 	Genome; Humicola; Sugarcane bagasse; Transcriptome.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/226063/1/Insights-into-the-Lignocellulose-Degrading-Enzyme-System-2021.pdf	
Registro original: 	Embrapa Agroenergia (CNPAE)	
Exemplares: 	CNPAE - (3942-1) - UPC - AP - DD -  -  -  - DIS