Data/hora: 19/06/2024 23:14:47

Biblioteca(s): | Embrapa Semiárido.
Data corrente: | 04/03/1998
Data da última atualização: | 16/06/2017
Autoria: | SZASZ, A.; MITYKO, J. L.; ANDRASFALVY, A.; FARI, M.
Título: | Methodological and genetic aspects of in vitro plant regeneration and genetic transformation of the recalcitrant pepper (capsicum annuum L.).
Ano de publicação: | 1997
Fonte/Imprenta: | Acta Horticulturae, Leuven, n. 447, p.365-366, 1997.
Idioma: | Inglês
Notas: | Edição do Proceedings of the 3 International Symposium on in Vitro Culture and Horticultural Breeding, Jerusalem, Israel, oct. 1996.
Conteúdo: | The transfer of the exccellent regeneration capacity found in inbred line No. 40017 - 13 was aaattempted in commercial, non regenerable pepper varieties by sexual crossing. The obtained hybrids were genetically stabilized by the creation of dihaploid lines. results obtained in these experiments pr oved it is a way to improve the very poor regeneration ability ofparticular pepper varieties. Genetic transformation experiments were carried out on  cotyledon and rooted hypocotyl explants of the No 40017 R -13 inbred line using a disamed Agrobacterium strain. the key step in the Agrobacterium mediated genetic transformation of pepper using cotyledons as explants was the elongation of the shoots surving the first kanamycin selection. It was observed that cefotaxim used to eliminate the bacteria has a phytohormone effect detrimental to this regeneration step. Interactions between antibiotics (cefotaxim, amoxycillin) and phytohormones (BAP, TDZ) during the regeneration process in cotyledon explants were revealed.
Palavras-Chave: | Regeneracao in vitro.
Thesagro: | Capsicum Annuum; Genética; Pimentão; Planta; Reprodução Vegetal.
Thesaurus Nal: | genetic transformation; in vitro regeneration; pepper.
Categoria do assunto: | A Sistemas de Cultivo
Registro original: | Embrapa Semiárido (CPATSA)
Exemplares: | CPATSA - (8316-1) - ADD - AP - PP -  -  -  - DIS