Data/hora: 19/06/2024 19:36:33

Biblioteca(s): 	Embrapa Semiárido.	
Data corrente: 	04/03/1998	
Data da última atualização: 	16/06/2017	
Autoria: 	MITYKO, J.; FARI, M.	
Título: 	Problems and results of doubled haploid plant production in pepper (Capsicum annum L.) via anther - and microspore culture.	
Ano de publicação: 	1997	
Fonte/Imprenta: 	Acta Horticulturae, Leuven, n. 447, p.281-287, 1997.	
Idioma: 	Inglês	
Notas: 	Edição do Proceedings of the 3 International Symposium on in Vitro Culture and Horticultural Breeding, Jerusalem, Israel, oct. 1996.	
Conteúdo: 	More than 500 pepper varieties, breeding lines and F1 hybrids were investigated in anther culture. A decreasing androgenic response was obtained in wax-type, dark green blocky-type, tomato-shaped and Cayenne;chilli-type peppers (from 76 to 0 plants/100 cultured anthers). Although bell peppers generated a high? and reproducible response, spice peppers proved to be less or non-responsive. An in vitro colchicine treatment was applied to the haploid regenerated plants for chromosome doubling with a 75% rediplolidization rate. One responsive (Feherozon) and two poorly responsive (No. 40017 R-13 and No. 40017 RK-II) genotypes were selected for microspore culture experiments. Three basic culture media were used with different carbohydrate and growth regulator supplements. the highest frequency of microspore divisions was obtained on NLN-2-15 medium (modified LIchter, 1981 medium containing 2% saccharose, 0,5 mg/l NAA and 0,5 mg/l BA). Sporophytic divisions resulted in some multicellular pollens, but enbryo development from these structures has not been obtained ywt. Stigma co-culture, exine wall degestioni and the colchicine effect on microspore divisions were investigated. Although neither the stigma co-culture nor the enzyme treatment could increase the cell division frequency , the positive effect of the drug (25 mg;l for 24 h) on the microspore development was clear.	
Palavras-Chave: 	Colchicina in vitro; In vitro colchicine; Production; Treatment.	
Thesagro: 	Capsicum Annuum; Cultura de Tecido; Melhoramento; Pimentão; Produção; Tratamento.	
Thesaurus Nal: 	breeding; pepper; tissue culture.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Semiárido (CPATSA)	
Exemplares: 	CPATSA - (7605-1) - ADD - AP - PP -  -  -  - DIS