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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
20/10/2004 |
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Data da última atualização: |
26/10/2004 |
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Autoria: |
FALEIRO, F. G.; YAMADA, M. M.; LOPES, U. V.; FALEIRO, A. S. G.; BAHIA, R. de C. S.; GOMES, L. M. C.; SANTOS, R. C.; SANTOS, R. F. dos. |
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Título: |
Genetic similarity of Theobroma cacao L. accessions maintained in duplicate at the cacao research center germplasm collection based on RAPD markers. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 2, n. 3, p. 439-443, Sept. 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to use RAPD markers to analyze the genetic similarity of 18 accessions maintained in 37 rows in the germplasm collection of Cacao Research Center (CEPEC/CEPLAC), Itabuna, Brazil. Within each row a plant was chosen at random, from which genomic DNA was extracted. DNA sample of each row was amplified by the RAPD. Fifty one RAPD markers were generated, and then transformed to a matrix of binary data. These binary data were used to estimate genetic similarities and to perform cluster analyses. Genetic similarities of 0.70, 0.94 and 0.96 were observed between plants of the accessions RB 29, Ca 1 and C. Sul 4, respectively. The mistakes in the identification of accessions in different germplasm collections seems to be more common than expected. Also, considering the great importance of the germplasm collections, an international effort would be necessary for the characterization of all important cacao germplasm collections around the world. |
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Palavras-Chave: |
Banco; Cocoa breeding; DNA analysis; Molecular markers. |
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Thesagro: |
Cacau; Germoplasma. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01828naa a2200277 a 4500
001 1212483
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008 2002 bl uuuu u00u1 u #d
100 1 $aFALEIRO, F. G.
245 $aGenetic similarity of Theobroma cacao L. accessions maintained in duplicate at the cacao research center germplasm collection based on RAPD markers.
260 $c2002
520 $aThe objective of this work was to use RAPD markers to analyze the genetic similarity of 18 accessions maintained in 37 rows in the germplasm collection of Cacao Research Center (CEPEC/CEPLAC), Itabuna, Brazil. Within each row a plant was chosen at random, from which genomic DNA was extracted. DNA sample of each row was amplified by the RAPD. Fifty one RAPD markers were generated, and then transformed to a matrix of binary data. These binary data were used to estimate genetic similarities and to perform cluster analyses. Genetic similarities of 0.70, 0.94 and 0.96 were observed between plants of the accessions RB 29, Ca 1 and C. Sul 4, respectively. The mistakes in the identification of accessions in different germplasm collections seems to be more common than expected. Also, considering the great importance of the germplasm collections, an international effort would be necessary for the characterization of all important cacao germplasm collections around the world.
650 $aCacau
650 $aGermoplasma
653 $aBanco
653 $aCocoa breeding
653 $aDNA analysis
653 $aMolecular markers
700 1 $aYAMADA, M. M.
700 1 $aLOPES, U. V.
700 1 $aFALEIRO, A. S. G.
700 1 $aBAHIA, R. de C. S.
700 1 $aGOMES, L. M. C.
700 1 $aSANTOS, R. C.
700 1 $aSANTOS, R. F. dos
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 2, n. 3, p. 439-443, Sept. 2002.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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