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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
16/10/2015 |
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Data da última atualização: |
16/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIANA, J. F. C.; CARRIJO, J.; FREITAS, C. G.; PAUL, A.; ALCARAZ, J.; LACORTE, C. C.; MIGLIOLO, L.; ANDRADE, C. A.; FALCAO, R.; SANTOS, N. C.; GONÇALVES, S.; OTERO-GONZÁLES, A. J.; KHADEMHOSSEINI, A.; DIAS, S. C.; FRANCO, O. L. |
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Afiliação: |
Juliane F. C. Viana, UnB; Jéssica Carrijo, UCB; Camila G. Freitas, UCB; Arghya Paul, Massachusetts Institute of Technology, Cambridge, USA; Jarib Alcaraz, Universidade Federal de Pernambuco; CRISTIANO CASTRO LACORTE, CENARGEN; Ludovico Migliolo, UCB; César A. Andrade, Universidade Federal de Pernambuco; ROSANA FALCAO, CENARGEN; Nuno C. Santos, Universidade de Lisboa; Sónia Gonçalves, Universidade de Lisboa; Anselmo J. Otero-González, Universidad de La Habana; Ali Khademhosseini, Massachusetts Institute of Technology, Cambridge, USA; Simoni C. Dias, UCB; Octávio L. Franco, UnB. |
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Título: |
Antifungal nanofibers made by controlled release of sea animal derived peptide. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Nanoscale, v. 7, p. 6238-6246, 2015. |
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Idioma: |
Português |
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Conteúdo: |
Candida albicans is a common human-pathogenic fungal species with the ability to cause several diseases including surface infections. Despite the clear difficulties of Candida control, antimicrobial peptides (AMPs) have emerged as an alternative strategy for fungal control. In this report, different concentrations of antifungal Cm-p1 (Cencritchis muricatus peptide 1) were electrospun into nanofibers for drug delivery. The nanofibers were characterized by mass spectrometry confirming the presence of the peptide on the scaffold. Atomic force microscopy and scanning electronic microscopy were used to measure the diameters, showing that Cm-p1 affects fiber morphology as well as the diameter and scaffold thickness. The Cm-p1 release behavior from the nanofibers demonstrated peptide release from 30 min to three days, leading to effective yeast control in the first 24 hours. Moreover, the biocompatibility of the fibers were evaluated through a MTS assay as well as ROS production by using a HUVEC model, showing that the fibers do not affect cell viability and only nanofibers containing 10% Cm-p1?PVA improved ROS generation. In addition, the secretion of pro-inflammatory cytokines IL-6 and TNF-? by the HUVECs was also slightly modified by the 10% Cm-p1?PVA nanofibers. In conclusion, the electrospinning technique applied here allowed for the manufacture of biodegradable biomimetic nanofibrous extracellular membranes with the ability to control fungal infection. |
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Palavras-Chave: |
Controle de fungos; Fungos; Nanofibras. |
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Thesagro: |
Cândida Albicans. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156226/1/c5nr00767d.pdf
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Marc: |
LEADER 02381naa a2200337 a 4500
001 2026638
005 2023-03-16
008 2015 bl uuuu u00u1 u #d
100 1 $aVIANA, J. F. C.
245 $aAntifungal nanofibers made by controlled release of sea animal derived peptide.$h[electronic resource]
260 $c2015
520 $aCandida albicans is a common human-pathogenic fungal species with the ability to cause several diseases including surface infections. Despite the clear difficulties of Candida control, antimicrobial peptides (AMPs) have emerged as an alternative strategy for fungal control. In this report, different concentrations of antifungal Cm-p1 (Cencritchis muricatus peptide 1) were electrospun into nanofibers for drug delivery. The nanofibers were characterized by mass spectrometry confirming the presence of the peptide on the scaffold. Atomic force microscopy and scanning electronic microscopy were used to measure the diameters, showing that Cm-p1 affects fiber morphology as well as the diameter and scaffold thickness. The Cm-p1 release behavior from the nanofibers demonstrated peptide release from 30 min to three days, leading to effective yeast control in the first 24 hours. Moreover, the biocompatibility of the fibers were evaluated through a MTS assay as well as ROS production by using a HUVEC model, showing that the fibers do not affect cell viability and only nanofibers containing 10% Cm-p1?PVA improved ROS generation. In addition, the secretion of pro-inflammatory cytokines IL-6 and TNF-? by the HUVECs was also slightly modified by the 10% Cm-p1?PVA nanofibers. In conclusion, the electrospinning technique applied here allowed for the manufacture of biodegradable biomimetic nanofibrous extracellular membranes with the ability to control fungal infection.
650 $aCândida Albicans
653 $aControle de fungos
653 $aFungos
653 $aNanofibras
700 1 $aCARRIJO, J.
700 1 $aFREITAS, C. G.
700 1 $aPAUL, A.
700 1 $aALCARAZ, J.
700 1 $aLACORTE, C. C.
700 1 $aMIGLIOLO, L.
700 1 $aANDRADE, C. A.
700 1 $aFALCAO, R.
700 1 $aSANTOS, N. C.
700 1 $aGONÇALVES, S.
700 1 $aOTERO-GONZÁLES, A. J.
700 1 $aKHADEMHOSSEINI, A.
700 1 $aDIAS, S. C.
700 1 $aFRANCO, O. L.
773 $tNanoscale$gv. 7, p. 6238-6246, 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
15/06/2023 |
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Data da última atualização: |
15/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
VILVERT, J. C.; SANTOS, L. F. dos; CARDOSO, A. D.; LOPES, P. R. C.; AMARANTE, C. V. T. do; FREITAS, S. T. de. |
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Afiliação: |
JOÃO CLAUDIO VILVERT, State University of Southwestern Bahia, Vitória da Conquista-BA; LUANA FERREIRA DOS SANTOS; ADRIANA DIAS CARDOSO, State University of Southwestern Bahia, Vitória da Conquista-BA; PAULO ROBERTO COELHO LOPES, CPATSA; CASSANDRO VIDAL TALAMINI DO AMARANTE, State University of Santa Catarina, Lages-SC; SERGIO TONETTO DE FREITAS, CPATSA. |
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Título: |
Non-destructive assessment of quality traits in apples and pears using near infrared spectroscopy and chemometrics. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Revista Brasileira de Fruticultura, v. 45, e-969, Mar./Apr. 2023. |
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DOI: |
https://dx.doi.org/10.1590/0100-29452023969 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to evaluate the performance of a hand-held NIR spectrometer for non-destructive quality analysis of apples and pears produced in the Brazilian Semi-arid region. NIR spectra were acquired with a portable spectrometer in the wavelength range of 750–1065 nm and reference analyses of dry matter content (DMC) and soluble solids content (SSC) were measured weekly during 10 weeks of storage at 0.5 °C. Spectra were pre-processed with standard normal variate and used to develop DMC and SSC models using partial least squares re- gression with full cross-validation. The models were validated using data not included in the calibration. Satisfactory prediction results were obtained for SSC in apples (R² = 0.58) and pears (R² = 0.55), and for DMC in apples (R² = 0.55) and pears (R² = 0.65). All prediction models showed a relative root mean square error of prediction lower than 8%. These findings indicate that the NIR spectrometer is a promising tool to be used for a rapid and non-destructive determination of internal quality traits in apples and pears. |
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Palavras-Chave: |
Espectrômetro portátil NIR; Qualidade do fruto; Quimiometria; Regressão de mínimos quadrados parciais; Regressão multivariada; Sólidos solúveis. |
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Thesagro: |
Maçã; Matéria Seca; Pêra; Pós-Colheita. |
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Thesaurus NAL: |
Apples; Dry matter intake; Fruit quality; Pears; Total soluble solids. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154443/1/Non-destructive-assessment-of-quality-traits-in-apples-and-pears-using-near-infrared-spectroscopy-and-chemometrics-2023.pdf
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Marc: |
LEADER 02248naa a2200373 a 4500
001 2154443
005 2023-06-15
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://dx.doi.org/10.1590/0100-29452023969$2DOI
100 1 $aVILVERT, J. C.
245 $aNon-destructive assessment of quality traits in apples and pears using near infrared spectroscopy and chemometrics.$h[electronic resource]
260 $c2023
520 $aThe objective of this study was to evaluate the performance of a hand-held NIR spectrometer for non-destructive quality analysis of apples and pears produced in the Brazilian Semi-arid region. NIR spectra were acquired with a portable spectrometer in the wavelength range of 750–1065 nm and reference analyses of dry matter content (DMC) and soluble solids content (SSC) were measured weekly during 10 weeks of storage at 0.5 °C. Spectra were pre-processed with standard normal variate and used to develop DMC and SSC models using partial least squares re- gression with full cross-validation. The models were validated using data not included in the calibration. Satisfactory prediction results were obtained for SSC in apples (R² = 0.58) and pears (R² = 0.55), and for DMC in apples (R² = 0.55) and pears (R² = 0.65). All prediction models showed a relative root mean square error of prediction lower than 8%. These findings indicate that the NIR spectrometer is a promising tool to be used for a rapid and non-destructive determination of internal quality traits in apples and pears.
650 $aApples
650 $aDry matter intake
650 $aFruit quality
650 $aPears
650 $aTotal soluble solids
650 $aMaçã
650 $aMatéria Seca
650 $aPêra
650 $aPós-Colheita
653 $aEspectrômetro portátil NIR
653 $aQualidade do fruto
653 $aQuimiometria
653 $aRegressão de mínimos quadrados parciais
653 $aRegressão multivariada
653 $aSólidos solúveis
700 1 $aSANTOS, L. F. dos
700 1 $aCARDOSO, A. D.
700 1 $aLOPES, P. R. C.
700 1 $aAMARANTE, C. V. T. do
700 1 $aFREITAS, S. T. de
773 $tRevista Brasileira de Fruticultura$gv. 45, e-969, Mar./Apr. 2023.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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