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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
08/12/2020 |
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Data da última atualização: |
08/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CATTANI, A. M.; FALAVIGNA, V. da S.; SILVEIRA, C. P.; BUFFON, V.; MARASCHIN, F. dos S.; PASQUALI, G.; REVERS, L. F. |
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Afiliação: |
AMANDA MALVESSI CATTANI, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; VÍTOR DA SILVEIRA FALAVIGNA, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Köln, Germany; CAROLINA PEREIRA SILVEIRA, Laboratory of Plant Molecular Genetics, Embrapa Uva e Vinho, Bento Gonçalves, RS, Brazil; VANESSA BUFFON, CNPUV; FELIPE DOS SANTOS MARASCHIN, Departament of Botany, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; GIANCARLO PASQUALI, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 39, p. 1687-1703, 2020. |
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DOI: |
10.1007_s00299-020-02595-z.pdf |
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Idioma: |
Inglês |
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Conteúdo: |
Key Message Cytokinin together with MdoBRR1, MdoBRR8 and MdoBRR10 genes participate in the downregulation of MdoDAM1, contributing to the transition from endo- to ecodormancy in apple buds. Abstract The fnal step of cytokinin (CK) signaling pathway culminates in the activation of type-B response regulators (BRRs), important transcriptional factors in the modulation of CK-responsive genes. In this study, we performed a genomewide analysis aiming to identify apple BRR family members and understand their involvement in bud dormancy control. The investigation identifed ten MdoBRR protein-coding genes. A higher expression of three MdoBRR (MdoBRR1, MdoBRR9 and MdoBRR10) was observed in dormant buds in comparison to other developmental stages. Interestingly, in ecodormant buds these three MdoBRR genes were upregulated in a CK-dependent manner. Transcription profles, determined during dormancy cycle under feld and artifcially controlled conditions, revealed that MdoBRR1 and MdoBRR8 played important roles in the transition from endo- to ecodormancy, probably mediated by endogenous CK stimuli. The expression of MdoBRR7, MdoBRR9, and MdoBRR10 was induced in ecodormant buds exposed to warm temperatures, indicating a putative role in growth resumption after chilling requirement fulfllment. Contrasting expression patternsin vivo between MdoBRRs and MdoDAM1, an essential dormancy establishment regulator, were observed during dormancy cycle and in CK-treated buds. Thereafter, in vivo transactivation assays showed that CK stimuli combined with transient overexpression of MdoBRR1, MdoBRR8, and MdoBRR10 resulted in downregulation of the reporter gene gusA driven by the MdoDAM1 promoter. These pieces of evidences point to the integration of CK-triggered responses through MdoBRRs that are able to downregulate MdoDAM1, contributing to dormancy release in apple. Keywords MdoBRR · Cytokinin · Bud dormancy · MdoDAM1 · Apple MenosKey Message Cytokinin together with MdoBRR1, MdoBRR8 and MdoBRR10 genes participate in the downregulation of MdoDAM1, contributing to the transition from endo- to ecodormancy in apple buds. Abstract The fnal step of cytokinin (CK) signaling pathway culminates in the activation of type-B response regulators (BRRs), important transcriptional factors in the modulation of CK-responsive genes. In this study, we performed a genomewide analysis aiming to identify apple BRR family members and understand their involvement in bud dormancy control. The investigation identifed ten MdoBRR protein-coding genes. A higher expression of three MdoBRR (MdoBRR1, MdoBRR9 and MdoBRR10) was observed in dormant buds in comparison to other developmental stages. Interestingly, in ecodormant buds these three MdoBRR genes were upregulated in a CK-dependent manner. Transcription profles, determined during dormancy cycle under feld and artifcially controlled conditions, revealed that MdoBRR1 and MdoBRR8 played important roles in the transition from endo- to ecodormancy, probably mediated by endogenous CK stimuli. The expression of MdoBRR7, MdoBRR9, and MdoBRR10 was induced in ecodormant buds exposed to warm temperatures, indicating a putative role in growth resumption after chilling requirement fulfllment. Contrasting expression patternsin vivo between MdoBRRs and MdoDAM1, an essential dormancy establishment regulator, were observed during dormancy cycle and in CK-treated buds. Thereafter, in vivo transa... Mostrar Tudo |
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Palavras-Chave: |
Bud dormancy; Cytokinin; MdoBRR; MdoDAM1. |
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Thesaurus Nal: |
Apples. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218972/1/10.1007-s00299-020-02595-z.pdf
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Marc: |
LEADER 02741naa a2200265 a 4500
001 2127859
005 2020-12-08
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007_s00299-020-02595-z.pdf$2DOI
100 1 $aCATTANI, A. M.
245 $aType-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.$h[electronic resource]
260 $c2020
520 $aKey Message Cytokinin together with MdoBRR1, MdoBRR8 and MdoBRR10 genes participate in the downregulation of MdoDAM1, contributing to the transition from endo- to ecodormancy in apple buds. Abstract The fnal step of cytokinin (CK) signaling pathway culminates in the activation of type-B response regulators (BRRs), important transcriptional factors in the modulation of CK-responsive genes. In this study, we performed a genomewide analysis aiming to identify apple BRR family members and understand their involvement in bud dormancy control. The investigation identifed ten MdoBRR protein-coding genes. A higher expression of three MdoBRR (MdoBRR1, MdoBRR9 and MdoBRR10) was observed in dormant buds in comparison to other developmental stages. Interestingly, in ecodormant buds these three MdoBRR genes were upregulated in a CK-dependent manner. Transcription profles, determined during dormancy cycle under feld and artifcially controlled conditions, revealed that MdoBRR1 and MdoBRR8 played important roles in the transition from endo- to ecodormancy, probably mediated by endogenous CK stimuli. The expression of MdoBRR7, MdoBRR9, and MdoBRR10 was induced in ecodormant buds exposed to warm temperatures, indicating a putative role in growth resumption after chilling requirement fulfllment. Contrasting expression patternsin vivo between MdoBRRs and MdoDAM1, an essential dormancy establishment regulator, were observed during dormancy cycle and in CK-treated buds. Thereafter, in vivo transactivation assays showed that CK stimuli combined with transient overexpression of MdoBRR1, MdoBRR8, and MdoBRR10 resulted in downregulation of the reporter gene gusA driven by the MdoDAM1 promoter. These pieces of evidences point to the integration of CK-triggered responses through MdoBRRs that are able to downregulate MdoDAM1, contributing to dormancy release in apple. Keywords MdoBRR · Cytokinin · Bud dormancy · MdoDAM1 · Apple
650 $aApples
653 $aBud dormancy
653 $aCytokinin
653 $aMdoBRR
653 $aMdoDAM1
700 1 $aFALAVIGNA, V. da S.
700 1 $aSILVEIRA, C. P.
700 1 $aBUFFON, V.
700 1 $aMARASCHIN, F. dos S.
700 1 $aPASQUALI, G.
700 1 $aREVERS, L. F.
773 $tPlant Cell Reports$gv. 39, p. 1687-1703, 2020.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| Registros recuperados : 34 | |
| 4. |  | FALAVIGNA, V. da S.; PORTO, D. D.; REVERS, L. F.; PEZZOTTI, M. Análise global da expressão de genes Dof em videira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 12., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 8., 2014, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2014. p. 40| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 6. | | FALAVIGNA, V. da S.; PORTO, D. D.; BUFFON, V.; BEKER, S. A.; REVERS, L. F. Caracterização da dormência de gemas em macieira: obtenção de bibliotecas supressivas substrativas para identificação de genes associados ao requerimento de frio hibernal. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 7.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 3., 2009, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2009. p. 54.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 7. | | CATTANI, A. N.; FALAVIGNA, V. da S.; ARENHART, R. A.; PASQUALI, G.; REVERS, L. F. Caracterização estrutural e funcional da subfamília gênica ARR-tipo B em macieira In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 19. (Embrapa Uva e Vinho. Documentos, 99)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | MIOTTO, Y. E.; CUSIN, R.; FALAVIGNA, V. da S.; PORTO, D. D.; REVERS, L. F. Identificação e análise de promotores de desidrinas no genoma da macieira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 9.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 5., 2011, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2011. p. 24. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 13. | | CUSIN, R.; SILVEIRA, C. P.; FALAVIGNA, V. da S.; ALVES, S. A. M.; REVERS, L. F.; MASCHIN, F. dos S. Transferring scab resistance to susceptible apple cultivars by virus-derived episomal plant plasmid expression. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 553.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 14. | | MIOTTO, Y. E.; FALAVIGNA, V. da S.; PORTO, D. D.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Identificação, classificação e caracterização transcricional das desidrinas de macieira. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, p. 620_623, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 16. | | FALAVIGNA, V. da S.; PORTO, D. D.; BUFFON, V.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Perfil transcricional em gemas dormentes de duas cultivares de macieira com requerimento de frio contrastante. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 10.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 6., 2012, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2012. p. 13. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 17. | | FALAVIGNA, V. da S.; MIOTTO, Y. E.; PORTO, D. D.; ANZANELLO, R.; FIALHO, F. B.; SANTOS, H. P. dos; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Análise da expressão de genes codificadores de desidrinas durante a dormência de gemas em macieira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 11.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 7., Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2013. p. 20. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 18. | | ALMEIDA, G. S. d'; BRBETON, M. C.; MARTINS, P. D.; FALAVIGNA, V. da S.; TURCHETTO-ZOLET, A. C.; REVERS, L. F.; PASQUALI, G. Characterization of eucalyptus grandis dof gene family of transcription factors. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 17. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 19. | | ALMEIDA, G. S. d’; BRETON, M. C.; MARTINS, P. D.; FALAVIGNA, V. da S.; TURCHETTO-ZOLET, A. C.; REVERS, L. F.; PASQUALI, G. Characterization of Eucalyptus grandis' DOF gene family of transcription factors. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 17. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 34 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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