Data/hora: 20/06/2024 13:49:11

Biblioteca(s): | Embrapa Agrossilvipastoril; Embrapa Meio Ambiente.
Data corrente: | 20/02/2017
Data da última atualização: | 02/03/2017
Autoria: | ROMÃO-DUMARESQ, A. S.; DOURADO, M. N.; FAVARO, L. C. de L.; MENDES, R.; FERREIRA, A.; ARAÚJO, W. L.
Afiliação: | ALINE SILVA ROMÃO-DUMARESQ; MANUELLA NÓBREGA DOURADO; LEIA CECILIA DE LIMA FAVARO, CNPAE; RODRIGO MENDES, CNPMA; ANDERSON FERREIRA, ESALQ-USP; WELINGTON LUIZ ARAÚJO, ICB-USP.
Título: | Diversity of cultivated fungi associated with conventional and transgenic sugarcane and the interaction between endophytic trichoderma virens and host plant.
Ano de publicação: | 2016
Fonte/Imprenta: | Plos One, v. 11, n. 7, p. e0158974, 2016.
Idioma: | Inglês
Conteúdo: | Abstract: Plant-associated fungi are considered a vast source for biotechnological processes whose potential has been poorly explored. The interactions and diversity of sugarcane, one of the most important crops in Brazil, have been rarely studied, mainly concerning fungal communities and their interactions with transgenic plants. Taking this into consideration, the purpose of this study was, based on culture dependent strategy, to determine the structure and diversity of the fungal community (root endophytes and rhizosphere) associated with two varieties of sugarcane, a non-genetically modified (SP80-1842) variety and its genetically modified counterpart (IMI-1, expressing imazapyr herbicide resistance). For this, the sugarcane varieties were evaluated in three sampling times (3, 10 and 17 months after planting) under two crop management (weeding and herbicide treatments). In addition, a strain of Trichoderma virens, an endophyte isolated from sugarcane with great potential as a biological control, growth promotion and enzyme production agent, was selected for the fungal-plant interaction assays. The results of the isolation, characterization and evaluation of fungal community changes showed that the sugarcane fungal community is composed of at least 35 different genera, mostly in the phylum Ascomycota. Many genera are observed at very low frequencies among a few most abundant genera, some of which were isolated from specific plant sites (e.g., the roots or the rhizosphere). An assessment of the possible effects upon the fungal community showed that the plant growth stage was the only factor that significantly affected the community?s structure. Moreover, if transgenic effects are present, they may be minor compared to other natural sources of variation. The results of interaction studies using the Green fluorescent protein (GFP)-expressing T. virens strain T.v.223 revealed that this fungus did not promote any phenotypic changes in the host plant and was found mostly in the roots where it formed a dense mycelial cover and was able to penetrate the intercellular spaces of the root epidermis upper layers. The ability of T. virens to colonize plant roots suggests a potential for protecting plant health, inhibiting pathogens or inducing systemic resistance.
Palavras-Chave: | Cana-de-açúcar convencional; Cana-de-açúcar transgênica; Diversidade de fungos cultivados; Trichoderma virens endofítico.
Thesagro: | Planta Hospedeira.
Categoria do assunto: | --
Registro original: | Embrapa Meio Ambiente (CNPMA)

Exemplares:

CNPMA - (15307-1) - UPC - SP - DD -  -  -  - DIS
CPAMT - (773-1) - UPC - SP - DD -  -  -  - DIS