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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
31/05/2013 |
Data da última atualização: |
15/04/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OLIVEIRA, J. K.; MARTINS, G.; ESTEVES, L. V.; PENNA, B.; HAMOND, C.; FONSECA, J. F. da; RODRIGUES, A. L.; BRANDÃO, F. Z.; LILENBAUM, W. |
Afiliação: |
Faculty of Veterinary Medicine, Universidade Federal Fluminense - Niterói, RJ, Brazil; Laboratory of Veterinary Bacteriology, Universidade Federal Fluminense - Niterói, RJ, Brazil; Faculty of Veterinary Medicine, Universidade Federal Fluminense - Niterói, RJ, Brazil; Laboratory of Veterinary Bacteriology, Universidade Federal Fluminense - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; Faculty of Veterinary Medicine, Universidade Federal Fluminense - Niterói, RJ, Brazil; Faculty of Veterinary Medicine, Universidade Federal Fluminense - Niterói, RJ, Brazil; Laboratory of Veterinary Bacteriology, Universidade Federal Fluminense - Niterói, RJ, Brazil. |
Título: |
Changes in the vaginal flora of goats following a short-term protocol of oestrus induction and synchronisation with intravaginal sponges as well as their antimicrobial sensitivity. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Small Ruminant Research, Amsterdam, v. 113, n. 1, p. 162-166, June, 2013. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The aim of this study was to characterise the changes in the vaginal flora of goats subjected to the induction and synchronisation of oestrus using intravaginal sponges (IVSs). Mucous samples were collected from the vaginas of 32 Toggenburg goats using sterile swabs. Samples were obtained on the day that the IVSs inserted (D0), the day they were withdrawn (D6), and 24 h (D7), 48 h (D8), and a week (D13) after the sponges were withdrawn. The samples were transferred to the laboratory in transport medium, inoculated on 5% sheep blood agar, and incubated at 37 ?C (aerobic culture only). The susceptibility of the cultured bacteria to eight of the most frequently employed antimicrobial agents was determined using the disc diffusion method in Mueller?Hinton agar. Fewer colony-forming units (CFUs) were obtained from the samples that were taken before sponge insertion (D0) compared with those obtained on the other days. After the sponges were inserted, the number of CFUs obtained from the vaginal samples increased, reaching the highest value at the time of sponge withdrawal (D6), when 62.1% (18/29) of the samples yielded ?105 CFU/ml. At this time, more than one type (genus) of bacterial colony was detected. The most prevalent bacterium belonged to the genus Staphylococcus sp., except at the time of sponge withdrawal, when the most prevalent bacterium was Escherichia coli. The results of this study demonstrated that changes in the prevalent bacterial isolate and the number of CFUs in the vaginal flora of goats subjected to a short-term protocol of oestrus induction and synchronisation using IVS occurred while the IVSs were present and that there was a rapid re-establishment of the normal microbiota after the IVSs were removed. MenosAbstract: The aim of this study was to characterise the changes in the vaginal flora of goats subjected to the induction and synchronisation of oestrus using intravaginal sponges (IVSs). Mucous samples were collected from the vaginas of 32 Toggenburg goats using sterile swabs. Samples were obtained on the day that the IVSs inserted (D0), the day they were withdrawn (D6), and 24 h (D7), 48 h (D8), and a week (D13) after the sponges were withdrawn. The samples were transferred to the laboratory in transport medium, inoculated on 5% sheep blood agar, and incubated at 37 ?C (aerobic culture only). The susceptibility of the cultured bacteria to eight of the most frequently employed antimicrobial agents was determined using the disc diffusion method in Mueller?Hinton agar. Fewer colony-forming units (CFUs) were obtained from the samples that were taken before sponge insertion (D0) compared with those obtained on the other days. After the sponges were inserted, the number of CFUs obtained from the vaginal samples increased, reaching the highest value at the time of sponge withdrawal (D6), when 62.1% (18/29) of the samples yielded ?105 CFU/ml. At this time, more than one type (genus) of bacterial colony was detected. The most prevalent bacterium belonged to the genus Staphylococcus sp., except at the time of sponge withdrawal, when the most prevalent bacterium was Escherichia coli. The results of this study demonstrated that changes in the prevalent bacterial isolate and the number ... Mostrar Tudo |
Palavras-Chave: |
Estro; Flora vaginal; Induction; Intravaginal sponges; Oestrus synchronization; Raça Toggenburg; Vaginal flora. |
Thesagro: |
Cabra leiteira; Caprino; Ciclo estral; Indução; Reprodução animal; Sincronização do cio. |
Thesaurus Nal: |
Goats. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02925naa a2200385 a 4500 001 1959079 005 2015-04-15 008 2013 bl uuuu u00u1 u #d 100 1 $aOLIVEIRA, J. K. 245 $aChanges in the vaginal flora of goats following a short-term protocol of oestrus induction and synchronisation with intravaginal sponges as well as their antimicrobial sensitivity.$h[electronic resource] 260 $c2013 520 $aAbstract: The aim of this study was to characterise the changes in the vaginal flora of goats subjected to the induction and synchronisation of oestrus using intravaginal sponges (IVSs). Mucous samples were collected from the vaginas of 32 Toggenburg goats using sterile swabs. Samples were obtained on the day that the IVSs inserted (D0), the day they were withdrawn (D6), and 24 h (D7), 48 h (D8), and a week (D13) after the sponges were withdrawn. The samples were transferred to the laboratory in transport medium, inoculated on 5% sheep blood agar, and incubated at 37 ?C (aerobic culture only). The susceptibility of the cultured bacteria to eight of the most frequently employed antimicrobial agents was determined using the disc diffusion method in Mueller?Hinton agar. Fewer colony-forming units (CFUs) were obtained from the samples that were taken before sponge insertion (D0) compared with those obtained on the other days. After the sponges were inserted, the number of CFUs obtained from the vaginal samples increased, reaching the highest value at the time of sponge withdrawal (D6), when 62.1% (18/29) of the samples yielded ?105 CFU/ml. At this time, more than one type (genus) of bacterial colony was detected. The most prevalent bacterium belonged to the genus Staphylococcus sp., except at the time of sponge withdrawal, when the most prevalent bacterium was Escherichia coli. The results of this study demonstrated that changes in the prevalent bacterial isolate and the number of CFUs in the vaginal flora of goats subjected to a short-term protocol of oestrus induction and synchronisation using IVS occurred while the IVSs were present and that there was a rapid re-establishment of the normal microbiota after the IVSs were removed. 650 $aGoats 650 $aCabra leiteira 650 $aCaprino 650 $aCiclo estral 650 $aIndução 650 $aReprodução animal 650 $aSincronização do cio 653 $aEstro 653 $aFlora vaginal 653 $aInduction 653 $aIntravaginal sponges 653 $aOestrus synchronization 653 $aRaça Toggenburg 653 $aVaginal flora 700 1 $aMARTINS, G. 700 1 $aESTEVES, L. V. 700 1 $aPENNA, B. 700 1 $aHAMOND, C. 700 1 $aFONSECA, J. F. da 700 1 $aRODRIGUES, A. L. 700 1 $aBRANDÃO, F. Z. 700 1 $aLILENBAUM, W. 773 $tSmall Ruminant Research, Amsterdam$gv. 113, n. 1, p. 162-166, June, 2013.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registros recuperados : 33 | |
2. | | ZAMBRINI, F. N.; GUIMARÃES, J. D.; ESTEVES, L. V.; FONSECA, J. F. da. Cervix dilation and transcervical embryo recovery in cervical Santa Inês sheep. Animal Reproduction, v. 11, n. 3, p. 139, Jul./Sept. 2014. Proceedings of the 28. Annual Meeting of The Brazilian Embryo Technology Society - SBTE, Natal, RN, Brasil, ago. 2014.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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6. | | FONSECA, J. F. da; CRUZ, R. C.; MANCIO, A. B.; ESTEVES, L. V.; BRANDÃO, F. Z.; FACO, O. Comportamento estral, dinâmica ovulatória e descarga de muco cervical em cabras Toggenburg submetidas à indução de estro com diferentes gonadotrofinas. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012. SBTE 067. p. 361.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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7. | | ZAMBRINI, F. N.; GUIMARÃES, J. D.; ESTEVES, L. V.; CASTRO, A. C. R. de; FONSECA, J. F. da. Cervix dilation and transcervical embryo recovery in cervical Santa Inês sheep. Animal Reproduction, v. 11, n. 3, p. 139, 2014. Edição dos proceedings do 28º Annual Meeting of the Brazilian Embryo Technology Society, 2014, Natal.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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8. | | ZANBRINI, F. N.; GUIMARÃES, J. D.; ESTEVES, L. V.; CASTRO, A. C. R.; FONSECA, J. F. da. Cervix dilation and transcervical embryo recovery in cervical Santa Inês sheep. Animal Reproduction, v. 11, n. 3, p. 419, Jul./Sept. 2014. Abstract A139. Proceedings of the 28th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), August, 2014, Natal, RN, Brazil. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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9. | | PINTO, P. H. N.; FREITAS, J. A. de; BRANDÃO, F. Z.; ESTEVES, L. V.; FONSECA, J. F. da. Possíveis impactos do projeto: "Uso de sêmen resfriado e inseminação artificial em caprinos leiteiros na República de Cabo Verde". Synergismus scyentifica UTFPR, Pato Branco, v. 7, n. 1, p. 1-3, 2012. Anais do 15o. Simpósio Paranaense de Ovinocultura, 3o. Simpósio Paranaense de Caprinocultura, 3o. Simpósio Sul Brasileiro de Ovinos e Caprinos, Pato Branco, PR, out., 2011.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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10. | | PINTO, P. H. N.; FREITAS, J. A.; FONSECA, J. D.; PILE, E. A.; ESTEVES, L. V.; SOUZA, V. L.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Artificial insemination in Cape Verdean Goats with cooled semen stored for 24 or 48 Hours. Australian Journal of Basic and Applied Sciences, v. 8, n. 18, p. 473-478, Dec. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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11. | | OLIVEIRA, J. K.; MARTINS, G.; ESTEVES, L. V.; PENNA, B.; HAMOND, C.; FONSECA, J. F. da; RODRIGUES, A. L.; BRANDÃO, F. Z.; LILENBAUM, W. Changes in the vaginal flora of goats following a short-term protocol of oestrus induction and synchronisation with intravaginal sponges as well as their antimicrobial sensitivity. Small Ruminant Research, Amsterdam, v. 113, n. 1, p. 162-166, June, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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12. | | OLIVEIRA, J. S. K.; FONSECA, J. F. da; ESTEVES, L. V.; MARTINS, G.; PENNA, B.; LILENBAUM, W.; FIGUEIRA, L. M.; BRANDÃO, F. Z. Mudanças na biota vaginal de cabras submetidas a protocolo de indução de estro sincronizado. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 19., 2011, Recife. Anais... Belo Horizonte: CBRA, 2011. p. 227. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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13. | | LIMA, J. T. M.; FONSECA, J. F. da; ESTEVES, L. V.; PENEIRAS, A. B. V.; LIMA, M. S. D.; BRANDÃO, F. Z. Duration of estrus and time of ovulation in superovulated Santa Inês ewes. Reproduction, Fertility and Development, v. 25, n. 1, p. 151, 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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14. | | ARASHIRO, E. K. N.; FONSECA, J. F. da; HENRY, M.; FIGUEIRA, L. M.; MAGÃO, J. V. P.; OLIVEIRA, D. R.; ESTEVES, L. V.; BRANDÃO, F. Z. Efeito do protocolo de sincronização da primeira onda folicular sobre a resposta a superovulação em ovelhas da raça Santa Inês. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 18., 2009, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2009. p. 433. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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16. | | ESTEVES, L. V.; BRANDÃO, F. Z.; CRUZ, R. C.; SOUZA, J. M. G.; OBA, E.; FACO, O.; FONSECA, J. F. da. Reproductive parameters of dairy goats submitted to estrus synchronization with prostaglandin F2alfa associated or not to hCG at estrous onset. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 65, n. 6, p. 1585-1592, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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17. | | ESTEVES, L. V.; BRANDÃO, F. Z.; ZAMBRINI, F. N.; SIQUEIRA, L. G. B.; FERREIRA, M. I. C.; FONSECA, J. F. da. Superovulação e coleta de embriões pela via transcervical em cabras da raça Toggenburg durante a estação reprodutiva. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 20., 2013, Uberlandia. Anais... Belo Horizonte: CBRA, 2013. p. 57.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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18. | | ESTEVES, L. V.; BRANDÃO, F. Z.; ZAMBRINI, F. N.; SIQUEIRA, L. G. B.; FERREIRA, M. I. C.; FONSECA, J. F. da. Superovulação e coleta de embriões pela via transcervical em cabras da raça Toggenburg durante a estação reprodutiva. CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 20., 2013, Uberlandia. Anais... Belo Horizonte: CBRA, 2013. p. 57Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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19. | | FONSECA, J. F. da; ESTEVES, L. V.; ZAMBRINI, F. N.; FERREIRA, M. I. C.; SIQUEIRA, L. G. B.; BRANDÃO, F. Z. Superovulation and embryo recovery by transcervical technique in Toggenburg goats during seasonal anestrus. In: ANNUAL MEETING - AETE, 29., 2013, Istanbul, Turkey. Colloque cientifique. Istanbu: Association Europeenne de Transfert Embryonnaire, 2013. p. 130.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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20. | | CASTRO, A. C. R.; NUNES, Z. F. N.; GUIMARÃES, A. de S. L.; ESTEVES, L. V.; ARASHIRO, E. K. N.; FONSECA, J. F. da. Uso de diferentes tipos de estradiol para a transposição cervical e lavagem uterina em fêmeas Santa Inês. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 3., 2014, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2014. p. 35.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 33 | |
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