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| Registros recuperados : 20 | |
| Registros recuperados : 20 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
23/02/2018 |
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Data da última atualização: |
23/02/2018 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
PAES, R. de C. da S.; FONSECA JUNIOR, A. A.; MONTEIRO, L. A. R. C.; JARDIM, G. C.; PIOVEZAN, U.; HERRERA, H. M.; MAURO, R. de A.; VIEIRA-DA-MOTA, O. |
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Afiliação: |
Rita de Cássia da Silva Paes, Agência Estadual de Defesa Sanitária Animal e Vegetal - Iagro/MS; Agência Estadual de Defesa Sanitária Animal e Vegetal - Iagro/MS; Agência Estadual de Defesa Sanitária Animal e Vegetal - Iagro/MS; Agência Estadual de Defesa Sanitária Animal e Vegetal – Iagro/MS; UBIRATAN PIOVEZAN, CPATC; Agência Estadual de Defesa Sanitária Animal e Vegetal - Iagro/MS; RODINEY DE ARRUDA MAURO, CNPGC; Agência Estadual de Defesa Sanitária Animal e Vegetal - Iagro/MS. |
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Título: |
Serological and molecular investigation of the prevalence of Aujeszky?s disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Veterinary Microbiology, v.165, n. 3-4, p. 448-454, 2013 |
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Idioma: |
Português |
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Notas: |
Short communication |
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Conteúdo: |
The feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the wetlands of MS, Brazil. MenosThe feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the we... Mostrar Tudo |
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Palavras-Chave: |
Ensaio de seroneutralização; Feral swine; Seroneutralization assay. |
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Thesagro: |
ELISA; Glicoproteína; Suíno. |
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Thesaurus NAL: |
Enzyme-linked immunosorbent assay; Glycoproteins. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/173049/1/55-Serological-and-molecular-investigation-of-the-prevalence-of-Aujeszkys-disease-in-feral-swine-Sus-scrofa-in-the-subregions-of-the-Pantanal-wetland-Brazil-20.pdf
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Marc: |
LEADER 02513nam a2200301 a 4500
001 2088072
005 2018-02-23
008 2013 bl uuuu u00u1 u #d
100 1 $aPAES, R. de C. da S.
245 $aSerological and molecular investigation of the prevalence of Aujeszky?s disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil.$h[electronic resource]
260 $aVeterinary Microbiology, v.165, n. 3-4, p. 448-454, 2013$c2013
500 $aShort communication
520 $aThe feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the wetlands of MS, Brazil.
650 $aEnzyme-linked immunosorbent assay
650 $aGlycoproteins
650 $aELISA
650 $aGlicoproteína
650 $aSuíno
653 $aEnsaio de seroneutralização
653 $aFeral swine
653 $aSeroneutralization assay
700 1 $aFONSECA JUNIOR, A. A.
700 1 $aMONTEIRO, L. A. R. C.
700 1 $aJARDIM, G. C.
700 1 $aPIOVEZAN, U.
700 1 $aHERRERA, H. M.
700 1 $aMAURO, R. de A.
700 1 $aVIEIRA-DA-MOTA, O.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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