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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
14/04/2004 |
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Data da última atualização: |
14/04/2004 |
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Autoria: |
ESCOBAR, R. H; PALACIO, J. D; RANGEL, M. P; ROCA, W. M. |
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Título: |
Cassava cryopreservation of shoot tips through encapsulation-dehydration technique. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v.17, p.50, nov, 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cryopreservation germplasm collection requires simple conditions, low cost and guarantee of genetic integrity. The development of a simple and reliable method for cryopreservation would allow widespread use of the methodology. Cassava (Manihot esculenta Crantz) shoot tips were successfully cryopreseved using and encapsulation-dehydration technique. Cassava shoot tip were encapsulated in 3% sodium alginate and dropped into 0:1M cacl2. Them beads were pretreated in sucrose medium for 3 days, dehydrated slowly down to 15-20% water content and plunged directly in liquid nitrogen. Dehydrtation on silica gel was more consistent than in a flow chamber and is not room temperature dependente. We found that sucrose concentration and time dehydration could affect response after freezing. This protocol was tested with 3 recalcitrant cassava cultivars, resulting in increased shoot recovery rates. Encapsulation of shoot tips is being tested with a sub-core cassava collection of 64 genotypes. Later on, we will expand the range of genotypes to the entire core collection. |
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Categoria do assunto: |
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Marc: |
LEADER 01557naa a2200157 a 4500
001 1651863
005 2004-04-14
008 1998 bl uuuu u00u1 u #d
100 1 $aESCOBAR, R. H
245 $aCassava cryopreservation of shoot tips through encapsulation-dehydration technique.
260 $c1998
520 $aAbstract: Cryopreservation germplasm collection requires simple conditions, low cost and guarantee of genetic integrity. The development of a simple and reliable method for cryopreservation would allow widespread use of the methodology. Cassava (Manihot esculenta Crantz) shoot tips were successfully cryopreseved using and encapsulation-dehydration technique. Cassava shoot tip were encapsulated in 3% sodium alginate and dropped into 0:1M cacl2. Them beads were pretreated in sucrose medium for 3 days, dehydrated slowly down to 15-20% water content and plunged directly in liquid nitrogen. Dehydrtation on silica gel was more consistent than in a flow chamber and is not room temperature dependente. We found that sucrose concentration and time dehydration could affect response after freezing. This protocol was tested with 3 recalcitrant cassava cultivars, resulting in increased shoot recovery rates. Encapsulation of shoot tips is being tested with a sub-core cassava collection of 64 genotypes. Later on, we will expand the range of genotypes to the entire core collection.
700 1 $aPALACIO, J. D
700 1 $aRANGEL, M. P
700 1 $aROCA, W. M.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv.17, p.50, nov, 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 7 | |
| 1. |  | ESCOBAR, R. Formação de mudas de dendê. Trabalho apresentado no Curso de Atualização em Produção de Sementes e Mudas, 1982, Manaus. Não paginado.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 5. | | SOH, A. C.; MAYES, S.; ROBERTS, J.; SILVA, E. B. da; AMBLARD, P.; ALVARADO, A.; ALVARADO, J. H.; ESCOBAR, R.; SRITHARAN, K.; SUBRAMANIAM, M.; ARULANDOO, X. Elaeis oleifera x Elaeis guineensis interspecific hybrid improvement. In: SOH, A. C.; MAYES, S.; ROBERTS, J. A. (Ed.). Oil palm breeding: genetics and genomics. Boca Raton: CRS Press, 2017. p. 283-296.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 6. | | FERNANDES, P. B.; PORCIUNCULA, G. C. da; COSTA, J. A. A. da; GRUSKA, J. F.; FERREIRA, M. B.; ESCOBAR, R. B. de S. V. O temperamento de ovelhas do grupo genético pantaneiro em sistemas de manejo distintos. In: CONGRESSO DE PESQUISA E PÓS-GRADUAÇÃO DO CAMPUS RIO VERDE, 5., 2016, Rio Verde. Anais. Rio Verde: Instituto Federal Goiano, Campus Rio Verde, 2016. 2 f.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | PORCIUNCULA, G. C. da; FISCHER, V.; COSTA, J. A. A. da; FERNANDES, P. B.; GRUSKA, J. F.; FERREIRA, M. B.; ESCOBAR, R. B. de S. V.; GAMES, N. G. R. Teste de Arena para avaliação do temperamento de ovelhas pantaneiras. In: SIMPÓSIO DE OVINOCULTURA DA UEL, 4., [2016], Londrina. [Resumos...]. Londrina: UEL, [2016] 1 f.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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