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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/12/2017 |
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Data da última atualização: |
04/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ERGUN; SOUZA, F. V. D. |
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Afiliação: |
ERGUN KAYA, Mugla Sitki Kocman University; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Comparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, v.53, Issue 4,p 410?417,| August 2017. |
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ISSN: |
1475-2689 |
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DOI: |
10.1007/s11627-017-9837-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Conservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stability for all cryopreserved varieties. MenosConservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stab... Mostrar Tudo |
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Thesagro: |
Criopreservação. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02196naa a2200169 a 4500
001 2081361
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022 $a1475-2689
024 7 $a10.1007/s11627-017-9837-2$2DOI
100 1 $aERGUN
245 $aComparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers.$h[electronic resource]
260 $c2017
520 $aConservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stability for all cryopreserved varieties.
650 $aCriopreservação
700 1 $aSOUZA, F. V. D.
773 $tIn Vitro Cellular & Developmental Biology - Plant$gv.53, Issue 4,p 410?417,| August 2017.
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| 43. |  | SPADOTTO, C. A. Comportamento e destino ambiental de produtos fitossanitários. Botucatu : FEPAF, 2015 In: Avanços na tecnologia de aplicação de produtos fitossanitários [recurso eletrônico] / João Paulo Arantes Rodrigues da Cunha, Ulisses Rocha Antuniassi, Cleyton Batista de Alvarenga, editores. Botucatu : FEPAF, 2015.| Tipo: Capítulo em Livro Técnico-Científico |
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| 44. | | CONCEICAO, M. C. A. Composição florística de uma floresta de várzea no município de colares no Estado do Pará. In: CONGRESSO FLORESTAL BRASILEIRO, 6., 1990, Campos do Jordão. Florestas e meio ambiente: conservação e produção, patrimônio social: anais. São José dos Campos: SBS: SBF, 1990. v. 3, p. 757-766. Edição dos Anais do Congresso Florestal Brasileiro, 6., 1990, Campos do Jordão.| Tipo: Artigo em Anais de Congresso |
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| 46. | | VELOSO, C. A. C. Café conilon (Coffea canephora). In: CRAVO, M. da S.; VIÉGAS, I. de J. M.; BRASIL, E. C. (Ed.). Recomendações de adubação e calagem para o Estado do Pará. Belém, PA: Embrapa Amazônia Oriental, 2007. pt. 3, cap. 1.| Tipo: Capítulo em Livro Técnico-Científico |
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