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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
28/12/2015 |
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Data da última atualização: |
03/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEZERRA JUNIOR, R. Q.; ELOY, A. M. X.; PEREIRA, E. P.; FURTADO, J. R.; SOUZA, K. C. de; LIMA, A. R.; PINHEIRO, R. R.; ANDRIOLI, A.; TEIXEIRA, M. F. da S. |
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Afiliação: |
Rosivaldo Quirino Bezerra Júnior, Pós-graduação - Laboratório de Virologia (LABOVIR), Universidade Estadual do Ceará (UECE), Fortaleza, CE; ANGELA MARIA XAVIER ELOY, CNPC; Elizângela Pinheiro Pereira, Pós-graduação - Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE; JOAO RICARDO FURTADO, CNPC; Kelma Costa de Souza, Pós-graduação - Laboratório de Virologia (LABOVIR), Universidade Estadual do Ceará (UECE), Fortaleza, CE; ADRIANO RODRIGUES LIMA, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; ALICE ANDRIOLI, CNPC; Maria Fátima da Silva Teixeira. |
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Título: |
Avaliação das metaloproteinases de matriz no sangue de reprodutores caprinos naturalmente infectados com Artrite Encefalite Caprina na Região Semiárida do Brasil. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 43, pub. 1258, p. 1-7, 2015. |
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Idioma: |
Português |
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Conteúdo: |
[Evaluation of Matrix Metalloproteinases in Blood from Buck Naturally Infected with Caprine Arthritis Encephalitis in Semiarid Region of Brazil]. Background: Studies have attempted to understand the importance of metalloproteinase (MMPs) in the pathogenesis of diseases caused by lentiviruses, being the human immunodeficiency virus (HIV) the most investigated. Despite advances in studies with MMPs in others diseases, research correlating the presence and activity of gelatinases in animals affected by caprine arthritis encephalitis virus (CAEV), a lentiviruses, are incipient and there is a need for research aiming to understand the dynamic of these enzymes in animals infected and its relation to pathological condition. The aim of this study was to evaluate the presence and activity of the MMPs in blood serum of chronically infected bucks by CAEV. Materials, Methods & Results: The experiment was constituted by two groups (n = 5 each group). The first one was composed of five naturally infected bucks (4-5 years) and second group constituted of five seronegative bucks (3-4 years) for CAE. Serology was performed using the Western Blotting (WB) and confirmed by Polymerase Chain Reaction (PCR). These bucks belong to the experimental flock at Embrapa Goats and Sheep and the seropositive bucks were confirmed for CAE in the first two years. Blood samples were collected by puncturing the jugular vein from animals and evaluated by zymography (SDS-PAGE) using gelatin as substratum. Clinical examination was performed by evaluating the temperature (T), cardiac frequency (FC), respiratory frequency (FR) and clinical articular index (IAC). The IAC was calculated by measuring the circumference of carpal joint and metacarpal bone height (difference between greater extent carpal and metacarpal lesser extent). In infected and control groups were found molecular mass bands of 66 kDa (MMP-2), 72 kDa (pro-MMP-2), 86 kDa (MMP-9) and 92 kDa (pro-MMP-9). The correlation analysis between respiratory frequency (RF), cardiac frequency (CF) and temperature (T°C), with MMPs activity (pro-MMP-2 and MMP-2), showed a negative and not significant correlation for RF (r = -0.1285) and T (r = -0.2588) and positive for CF (r = 0.01038), but not significant. Using the gel densitometry analysis it was observed higher activity (P < 0.05) of the pro-MMP-2 and MMP-2 in seropositive group. Discussion: The caprine arthritis encephalitis (CAE) is a multisystem infectious disease that causes production losses when present in flocks, especially the occurrence of arthritis. The higher activity of the MMPs (MMP-2 and MMP-9) in infected group to CAE suggests a role of these proteases in the chronic inflammatory process in goats. It can be explained by the fact that matrix metalloproteinases are regarded to be the key role molecules in inflammation and are involved in pathophysiological remodeling of the vascular wall. The variable presence of active and latent forms of MMP-9 in our study combined to an increased in the seropositive group activity corroborates the findings of other studies. In this study, only one seropositive animal demonstrated apparent arthritis although showed similar MMPs (MMP-2 and MMP-9) activity to others in the seropositive group, leads us to suggest that all the seropositive animals were stricken with an inflammatory process. Although there was statistical difference between the groups regarding to MMPs activity, this was not reflected on clinical symptoms in chronically infecetd animals, requiring further studies on this subject. Menos[Evaluation of Matrix Metalloproteinases in Blood from Buck Naturally Infected with Caprine Arthritis Encephalitis in Semiarid Region of Brazil]. Background: Studies have attempted to understand the importance of metalloproteinase (MMPs) in the pathogenesis of diseases caused by lentiviruses, being the human immunodeficiency virus (HIV) the most investigated. Despite advances in studies with MMPs in others diseases, research correlating the presence and activity of gelatinases in animals affected by caprine arthritis encephalitis virus (CAEV), a lentiviruses, are incipient and there is a need for research aiming to understand the dynamic of these enzymes in animals infected and its relation to pathological condition. The aim of this study was to evaluate the presence and activity of the MMPs in blood serum of chronically infected bucks by CAEV. Materials, Methods & Results: The experiment was constituted by two groups (n = 5 each group). The first one was composed of five naturally infected bucks (4-5 years) and second group constituted of five seronegative bucks (3-4 years) for CAE. Serology was performed using the Western Blotting (WB) and confirmed by Polymerase Chain Reaction (PCR). These bucks belong to the experimental flock at Embrapa Goats and Sheep and the seropositive bucks were confirmed for CAE in the first two years. Blood samples were collected by puncturing the jugular vein from animals and evaluated by zymography (SDS-PAGE) using gelatin as substratum. Clinic... Mostrar Tudo |
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Palavras-Chave: |
Artrite Encefalite Caprina; CAE; CAEV; Metalloproteinase; Metaloproteinase; MMP-2; MMP-9; Proteases; Zimografia; Zymography. |
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Thesagro: |
Caprino; Doença animal; Protease. |
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Thesaurus Nal: |
Caprine arthritis encephalitis virus; Goats; Metalloproteins. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136223/1/cnpc-2015-Avaliacao-das-metaloproteinases.pdf
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Marc: |
LEADER 04766naa a2200409 a 4500
001 2032515
005 2019-04-03
008 2015 bl uuuu u00u1 u #d
100 1 $aBEZERRA JUNIOR, R. Q.
245 $aAvaliação das metaloproteinases de matriz no sangue de reprodutores caprinos naturalmente infectados com Artrite Encefalite Caprina na Região Semiárida do Brasil.$h[electronic resource]
260 $c2015
520 $a[Evaluation of Matrix Metalloproteinases in Blood from Buck Naturally Infected with Caprine Arthritis Encephalitis in Semiarid Region of Brazil]. Background: Studies have attempted to understand the importance of metalloproteinase (MMPs) in the pathogenesis of diseases caused by lentiviruses, being the human immunodeficiency virus (HIV) the most investigated. Despite advances in studies with MMPs in others diseases, research correlating the presence and activity of gelatinases in animals affected by caprine arthritis encephalitis virus (CAEV), a lentiviruses, are incipient and there is a need for research aiming to understand the dynamic of these enzymes in animals infected and its relation to pathological condition. The aim of this study was to evaluate the presence and activity of the MMPs in blood serum of chronically infected bucks by CAEV. Materials, Methods & Results: The experiment was constituted by two groups (n = 5 each group). The first one was composed of five naturally infected bucks (4-5 years) and second group constituted of five seronegative bucks (3-4 years) for CAE. Serology was performed using the Western Blotting (WB) and confirmed by Polymerase Chain Reaction (PCR). These bucks belong to the experimental flock at Embrapa Goats and Sheep and the seropositive bucks were confirmed for CAE in the first two years. Blood samples were collected by puncturing the jugular vein from animals and evaluated by zymography (SDS-PAGE) using gelatin as substratum. Clinical examination was performed by evaluating the temperature (T), cardiac frequency (FC), respiratory frequency (FR) and clinical articular index (IAC). The IAC was calculated by measuring the circumference of carpal joint and metacarpal bone height (difference between greater extent carpal and metacarpal lesser extent). In infected and control groups were found molecular mass bands of 66 kDa (MMP-2), 72 kDa (pro-MMP-2), 86 kDa (MMP-9) and 92 kDa (pro-MMP-9). The correlation analysis between respiratory frequency (RF), cardiac frequency (CF) and temperature (T°C), with MMPs activity (pro-MMP-2 and MMP-2), showed a negative and not significant correlation for RF (r = -0.1285) and T (r = -0.2588) and positive for CF (r = 0.01038), but not significant. Using the gel densitometry analysis it was observed higher activity (P < 0.05) of the pro-MMP-2 and MMP-2 in seropositive group. Discussion: The caprine arthritis encephalitis (CAE) is a multisystem infectious disease that causes production losses when present in flocks, especially the occurrence of arthritis. The higher activity of the MMPs (MMP-2 and MMP-9) in infected group to CAE suggests a role of these proteases in the chronic inflammatory process in goats. It can be explained by the fact that matrix metalloproteinases are regarded to be the key role molecules in inflammation and are involved in pathophysiological remodeling of the vascular wall. The variable presence of active and latent forms of MMP-9 in our study combined to an increased in the seropositive group activity corroborates the findings of other studies. In this study, only one seropositive animal demonstrated apparent arthritis although showed similar MMPs (MMP-2 and MMP-9) activity to others in the seropositive group, leads us to suggest that all the seropositive animals were stricken with an inflammatory process. Although there was statistical difference between the groups regarding to MMPs activity, this was not reflected on clinical symptoms in chronically infecetd animals, requiring further studies on this subject.
650 $aCaprine arthritis encephalitis virus
650 $aGoats
650 $aMetalloproteins
650 $aCaprino
650 $aDoença animal
650 $aProtease
653 $aArtrite Encefalite Caprina
653 $aCAE
653 $aCAEV
653 $aMetalloproteinase
653 $aMetaloproteinase
653 $aMMP-2
653 $aMMP-9
653 $aProteases
653 $aZimografia
653 $aZymography
700 1 $aELOY, A. M. X.
700 1 $aPEREIRA, E. P.
700 1 $aFURTADO, J. R.
700 1 $aSOUZA, K. C. de
700 1 $aLIMA, A. R.
700 1 $aPINHEIRO, R. R.
700 1 $aANDRIOLI, A.
700 1 $aTEIXEIRA, M. F. da S.
773 $tActa Scientiae Veterinariae$gv. 43, pub. 1258, p. 1-7, 2015.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
24/11/2016 |
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Data da última atualização: |
24/11/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PUGLIESI, G.; SANTOS, F. B.; LOPES, E.; NOGUEIRA, E.; MAIO, J. R. G.; BINELLI, M. |
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Afiliação: |
G. PUGLIESI, University of São Paulo; F. B. SANTOS, UNIVERSITY OF SÃO PAULO; E. LOPES, UNIVERSITY OF SÃO PAULO; ERIKLIS NOGUEIRA, CPAP; J. R. G. MAIO, OURO FINO SAÚDE ANIMAL; M. BINELLI., UNIVERSITY OF SÃO PAULO. |
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Título: |
Improved fertility in suckled beef cows ovulating large follicles or supplemented with long-acting progesterone after timed-AI. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Theriogenology, n. 85, n. 7, p. 1239-1248, April 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
We aimed to evaluate the effects and the interaction of size of the preovulatory follicle (POF) and long-acting progesterone (P4) supplementation after timed-AI on CL function and pregnancy success in beef cows. In experiment 1, ovulations of beef cows were synchronized starting on Day 10, and cows were split to receive sodium cloprostenol (large follicle group; LF; n ¼ 31) or nothing (small follicle group; SF; n ¼ 35). Ovulations were induced on Day 0, and cows were inseminated. Ovulated cows were assigned to receive placebo (LF/control group, n ¼ 14; and SF/control group, n ¼ 9) or 150 mg of long-acting P4 on Day 4.5 (LF/P4 group, n ¼ 13; and SF/P4 group, n ¼ 12). Diameter of POF, blood flow in POF wall, ovulation rate, and size and vascularization of CL were greater (P < 0.05) in LF group. In experiments 2 (unknown cyclic status) and 4 (noncycling), ovulations were synchronized, and beef cows received placebo or 150 mg of long-acting P4 on Day 4 after timed-artificial insemination. In experiment 2, pregnancy/AI (P/AI) did not differ (P > 0.1) between P4-treated (53.2%; 209/393) and control cows (56.2%; 219/390), but P/AI was greater in cows with a CL < 0.9 cm2 on Day 4 that were P4-treated (57.9%, 22/38) versus placebo-treated (40.4%, 21/52; P < 0.05). In Experiment 4, P/AI was greater (P < 0.05) in P4-treated cows (55.6%, 105/189 vs. 46.0%, 86/187). In Experiment 3, cyclic?suckled beef cows were treated as described in Experiment 1 to generate animals with small (SF; n ¼ 111) or large POF (LF; n ¼ 109), and subdivided to receive placebo or P4 on Day 4. POF size, ovulation rate, CL area, and P/AI were greater (P < 0.007) in the LF group. Pregnancy/AI in ovulated cows were lower (P ¼ 0.05) in the SF/control group (41.5%, 17/41) compared to LF/control group (62%, 31/50) and were similar for the SF/P4 group (55.6%, 25/45) and LF/P4 group (57%, 28/49) compared to others. In summary, smaller and less vascularized POF results in less functional CL and reduces ovulatory rate and P/AI in cyclic beef cows; the long-acting P4 injection on Day 4 after timed-artificial insemination may attenuate the negative effects of small POF/CL; and postovulatory P4 supplementation improved fertility in anestrous beef cattle. MenosWe aimed to evaluate the effects and the interaction of size of the preovulatory follicle (POF) and long-acting progesterone (P4) supplementation after timed-AI on CL function and pregnancy success in beef cows. In experiment 1, ovulations of beef cows were synchronized starting on Day 10, and cows were split to receive sodium cloprostenol (large follicle group; LF; n ¼ 31) or nothing (small follicle group; SF; n ¼ 35). Ovulations were induced on Day 0, and cows were inseminated. Ovulated cows were assigned to receive placebo (LF/control group, n ¼ 14; and SF/control group, n ¼ 9) or 150 mg of long-acting P4 on Day 4.5 (LF/P4 group, n ¼ 13; and SF/P4 group, n ¼ 12). Diameter of POF, blood flow in POF wall, ovulation rate, and size and vascularization of CL were greater (P < 0.05) in LF group. In experiments 2 (unknown cyclic status) and 4 (noncycling), ovulations were synchronized, and beef cows received placebo or 150 mg of long-acting P4 on Day 4 after timed-artificial insemination. In experiment 2, pregnancy/AI (P/AI) did not differ (P > 0.1) between P4-treated (53.2%; 209/393) and control cows (56.2%; 219/390), but P/AI was greater in cows with a CL < 0.9 cm2 on Day 4 that were P4-treated (57.9%, 22/38) versus placebo-treated (40.4%, 21/52; P < 0.05). In Experiment 4, P/AI was greater (P < 0.05) in P4-treated cows (55.6%, 105/189 vs. 46.0%, 86/187). In Experiment 3, cyclic?suckled beef cows were treated as described in Experiment 1 to generate animals with small (SF; n ¼... Mostrar Tudo |
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Thesagro: |
Gado de corte; Ovulacao; Reproducao animal. |
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Thesaurus NAL: |
Animal reproduction; beef; Beef cows; cows; Ovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03031naa a2200277 a 4500
001 2057066
005 2016-11-24
008 2016 bl uuuu u00u1 u #d
100 1 $aPUGLIESI, G.
245 $aImproved fertility in suckled beef cows ovulating large follicles or supplemented with long-acting progesterone after timed-AI.
260 $c2016
520 $aWe aimed to evaluate the effects and the interaction of size of the preovulatory follicle (POF) and long-acting progesterone (P4) supplementation after timed-AI on CL function and pregnancy success in beef cows. In experiment 1, ovulations of beef cows were synchronized starting on Day 10, and cows were split to receive sodium cloprostenol (large follicle group; LF; n ¼ 31) or nothing (small follicle group; SF; n ¼ 35). Ovulations were induced on Day 0, and cows were inseminated. Ovulated cows were assigned to receive placebo (LF/control group, n ¼ 14; and SF/control group, n ¼ 9) or 150 mg of long-acting P4 on Day 4.5 (LF/P4 group, n ¼ 13; and SF/P4 group, n ¼ 12). Diameter of POF, blood flow in POF wall, ovulation rate, and size and vascularization of CL were greater (P < 0.05) in LF group. In experiments 2 (unknown cyclic status) and 4 (noncycling), ovulations were synchronized, and beef cows received placebo or 150 mg of long-acting P4 on Day 4 after timed-artificial insemination. In experiment 2, pregnancy/AI (P/AI) did not differ (P > 0.1) between P4-treated (53.2%; 209/393) and control cows (56.2%; 219/390), but P/AI was greater in cows with a CL < 0.9 cm2 on Day 4 that were P4-treated (57.9%, 22/38) versus placebo-treated (40.4%, 21/52; P < 0.05). In Experiment 4, P/AI was greater (P < 0.05) in P4-treated cows (55.6%, 105/189 vs. 46.0%, 86/187). In Experiment 3, cyclic?suckled beef cows were treated as described in Experiment 1 to generate animals with small (SF; n ¼ 111) or large POF (LF; n ¼ 109), and subdivided to receive placebo or P4 on Day 4. POF size, ovulation rate, CL area, and P/AI were greater (P < 0.007) in the LF group. Pregnancy/AI in ovulated cows were lower (P ¼ 0.05) in the SF/control group (41.5%, 17/41) compared to LF/control group (62%, 31/50) and were similar for the SF/P4 group (55.6%, 25/45) and LF/P4 group (57%, 28/49) compared to others. In summary, smaller and less vascularized POF results in less functional CL and reduces ovulatory rate and P/AI in cyclic beef cows; the long-acting P4 injection on Day 4 after timed-artificial insemination may attenuate the negative effects of small POF/CL; and postovulatory P4 supplementation improved fertility in anestrous beef cattle.
650 $aAnimal reproduction
650 $abeef
650 $aBeef cows
650 $acows
650 $aOvulation
650 $aGado de corte
650 $aOvulacao
650 $aReproducao animal
700 1 $aSANTOS, F. B.
700 1 $aLOPES, E.
700 1 $aNOGUEIRA, E.
700 1 $aMAIO, J. R. G.
700 1 $aBINELLI, M.
773 $tTheriogenology$gn. 85, n. 7, p. 1239-1248, April 2016.
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