| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
14/06/2018 |
|
Data da última atualização: |
14/06/2018 |
|
Autoria: |
BRAGA, P. A. C.; GONÇALVES, J. L.; BARREIRO, J. R.; FERREIRA, C. R.; TOMAZI, T.; EBERLIN, M. N.; SANTOS, M. V. |
|
Afiliação: |
Patrícia A. C. Braga, Laboratório Thomson de Espectrometria de Massas, Instituto de Química, Universidade Estadual de Campinas - Unicamp; Juliano L. Gonçalves, Departamento de Nutrição e Produção Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP; Juliana R. Barreiro, Departamento de Nutrição e Produção Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP; Christina R. Ferreira, Laboratório Thomson de Espectrometria de Massas, Instituto de Química, Universidade Estadual de Campinas - Unicamp; Tiago Tomazi, Departamento de Nutrição e Produção Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP; Marcos N. Eberlin, Laboratório Thomson de Espectrometria de Massas, Instituto de Química, Universidade Estadual de Campinas - Unicamp; Marcos V. Santos, Departamento de Nutrição e Produção Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP. |
|
Título: |
Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 586-594, abril 2018 |
|
Idioma: |
Inglês |
|
Notas: |
Título em português: Rápida identificação de agentes causadores de mastite por espectrometria de massas MALDI-TOF. |
|
Conteúdo: |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been shown to be an alternative method for identification of bacteria via their protein profile spectra, being able to identify bacteria at the genus, species and even at subspecies level. With the aim of large-scale identification of pathogens causing mastitis by this platform, a total of 305 isolates of bacteria identified from cows with subclinical mastitis were analyzed by conventional microbiological culture (MC) as well as by MALDI-TOF MS coupled with Biotyper data processing. Approximately 89% of the identifications performed by MALDI-TOF MS were consistent with results obtained by MC. From the remaining isolates (11%), 6.3% of isolates were classified as misidentified (discordance for both genus and species level), and 4.7% showed identification agreement at the genus level but not at the species level, being classified as unidentified at species level. The disagreement results were mostly associated with identification of Streptococcus and Enterococcus species probably due to the narrow phenotypic similarity between these two genera. These disagreement results suggest that biochemical assays might be prone to identification errors and, MALDI-TOF MS therefore may be an alternative to overcome incorrect species-specific identification. Standard microbiological methods for bovine mastitis diagnosis are time consuming, laborious and prone to errors for some bacteria genera. In our study, we showed that MALDI-TOF MS coupled with Biotyper may be an alternative method for large-scale identification of bacteria isolated from milk samples compared to classical microbiological routine protocols. MenosMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been shown to be an alternative method for identification of bacteria via their protein profile spectra, being able to identify bacteria at the genus, species and even at subspecies level. With the aim of large-scale identification of pathogens causing mastitis by this platform, a total of 305 isolates of bacteria identified from cows with subclinical mastitis were analyzed by conventional microbiological culture (MC) as well as by MALDI-TOF MS coupled with Biotyper data processing. Approximately 89% of the identifications performed by MALDI-TOF MS were consistent with results obtained by MC. From the remaining isolates (11%), 6.3% of isolates were classified as misidentified (discordance for both genus and species level), and 4.7% showed identification agreement at the genus level but not at the species level, being classified as unidentified at species level. The disagreement results were mostly associated with identification of Streptococcus and Enterococcus species probably due to the narrow phenotypic similarity between these two genera. These disagreement results suggest that biochemical assays might be prone to identification errors and, MALDI-TOF MS therefore may be an alternative to overcome incorrect species-specific identification. Standard microbiological methods for bovine mastitis diagnosis are time consuming, laborious and prone to errors for some bacteria genera. I... Mostrar Tudo |
|
Palavras-Chave: |
Espectrometria de massas; Identificação rápida; MALDI-TOF MS; Mastite subclínica; Perfil proteico; Rapid identification; Subclinical mastitis. |
|
Thesagro: |
Bacteriose; Bovino; Mamite. |
|
Thesaurus Nal: |
Bovine mastitis; Cattle; Mass spectrometry; Pathogens; Peptide mapping. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178661/1/Rapid-identification-of-bovine-mastitis.pdf
|
|
Marc: |
LEADER 02926naa a2200385 a 4500
001 2092505
005 2018-06-14
008 2018 bl uuuu u00u1 u #d
100 1 $aBRAGA, P. A. C.
245 $aRapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Rápida identificação de agentes causadores de mastite por espectrometria de massas MALDI-TOF.
520 $aMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been shown to be an alternative method for identification of bacteria via their protein profile spectra, being able to identify bacteria at the genus, species and even at subspecies level. With the aim of large-scale identification of pathogens causing mastitis by this platform, a total of 305 isolates of bacteria identified from cows with subclinical mastitis were analyzed by conventional microbiological culture (MC) as well as by MALDI-TOF MS coupled with Biotyper data processing. Approximately 89% of the identifications performed by MALDI-TOF MS were consistent with results obtained by MC. From the remaining isolates (11%), 6.3% of isolates were classified as misidentified (discordance for both genus and species level), and 4.7% showed identification agreement at the genus level but not at the species level, being classified as unidentified at species level. The disagreement results were mostly associated with identification of Streptococcus and Enterococcus species probably due to the narrow phenotypic similarity between these two genera. These disagreement results suggest that biochemical assays might be prone to identification errors and, MALDI-TOF MS therefore may be an alternative to overcome incorrect species-specific identification. Standard microbiological methods for bovine mastitis diagnosis are time consuming, laborious and prone to errors for some bacteria genera. In our study, we showed that MALDI-TOF MS coupled with Biotyper may be an alternative method for large-scale identification of bacteria isolated from milk samples compared to classical microbiological routine protocols.
650 $aBovine mastitis
650 $aCattle
650 $aMass spectrometry
650 $aPathogens
650 $aPeptide mapping
650 $aBacteriose
650 $aBovino
650 $aMamite
653 $aEspectrometria de massas
653 $aIdentificação rápida
653 $aMALDI-TOF MS
653 $aMastite subclínica
653 $aPerfil proteico
653 $aRapid identification
653 $aSubclinical mastitis
700 1 $aGONÇALVES, J. L.
700 1 $aBARREIRO, J. R.
700 1 $aFERREIRA, C. R.
700 1 $aTOMAZI, T.
700 1 $aEBERLIN, M. N.
700 1 $aSANTOS, M. V.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 4, p. 586-594, abril 2018
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
17/11/2016 |
|
Data da última atualização: |
03/08/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
VEIGA, M. da; PANDOLFO, C. M.; BALBINOT JUNIOR, A. A.; DURIGON, L. |
|
Afiliação: |
MILTON DA VEIGA, EPAGRI; CARLA MARIA PANDOLFO, UNOESC; ALVADI ANTONIO BALBINOT JUNIOR, CNPSO; LEANDRO DURIGON, Engenheiro Agronomo. |
|
Título: |
Effects on soil and crop properties of forms of sowing, deferral intervals and fertilisation of the annual winter forage in a crop-livestock integration system. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Journal of Agricultural Science, v. 8, n. 5, p. 15-25, 2016. |
|
ISSN: |
1916-9760 |
|
DOI: |
10.5539/jas.v8n5p15 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The use of cropland to grow annual winter forages that are managed using direct grazing can affect the quality of the soil and the yield of summer crops grown in succession. This study aimed to evaluate the effect that the form of sowing (direct sowing an d sowing + harrowing), the deferral intervals (ungrazed and grazing stopped at 14 days and 28 days before and on the day of forage desiccation) and the fertilisation of the annual winter forage (with and without application of 8 m 3 ha-1 of poultry litter) had on the chemical properties of soil, resistance to penetration, residual forage biomass, soil cover and yield of corn and soybean from the third to the sixth year of experimentation. The forms of sowing did not affect the chemical properties of the soil, resistance to penetration, the residual forage biomass and the yield of the soybean and corn grown in rotation. The grazing of annual winter forage did not affect the chemical properties of soil and the yield of soybean and corn grown in succession, but residual forage bioma ss was reduced and resistance to penetration was increased with decreasing deferral intervals. In turn, the fertilisation of winter forages improved the quality of the chemical properties of the soil and increased the residual forage biomass and crop yields in most of the four years examined in this experiment. |
|
Palavras-Chave: |
Integração lavoura-pecuária. |
|
Thesagro: |
Milho; Solo. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02081naa a2200217 a 4500
001 2056508
005 2017-08-03
008 2016 bl uuuu u00u1 u #d
022 $a1916-9760
024 7 $a10.5539/jas.v8n5p15$2DOI
100 1 $aVEIGA, M. da
245 $aEffects on soil and crop properties of forms of sowing, deferral intervals and fertilisation of the annual winter forage in a crop-livestock integration system.$h[electronic resource]
260 $c2016
520 $aThe use of cropland to grow annual winter forages that are managed using direct grazing can affect the quality of the soil and the yield of summer crops grown in succession. This study aimed to evaluate the effect that the form of sowing (direct sowing an d sowing + harrowing), the deferral intervals (ungrazed and grazing stopped at 14 days and 28 days before and on the day of forage desiccation) and the fertilisation of the annual winter forage (with and without application of 8 m 3 ha-1 of poultry litter) had on the chemical properties of soil, resistance to penetration, residual forage biomass, soil cover and yield of corn and soybean from the third to the sixth year of experimentation. The forms of sowing did not affect the chemical properties of the soil, resistance to penetration, the residual forage biomass and the yield of the soybean and corn grown in rotation. The grazing of annual winter forage did not affect the chemical properties of soil and the yield of soybean and corn grown in succession, but residual forage bioma ss was reduced and resistance to penetration was increased with decreasing deferral intervals. In turn, the fertilisation of winter forages improved the quality of the chemical properties of the soil and increased the residual forage biomass and crop yields in most of the four years examined in this experiment.
650 $aMilho
650 $aSolo
653 $aIntegração lavoura-pecuária
700 1 $aPANDOLFO, C. M.
700 1 $aBALBINOT JUNIOR, A. A.
700 1 $aDURIGON, L.
773 $tJournal of Agricultural Science$gv. 8, n. 5, p. 15-25, 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
