| |
|
|
| Registros recuperados : 775 | |
| Registros recuperados : 775 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
19/03/2018 |
|
Data da última atualização: |
19/03/2018 |
|
Autoria: |
NOQUEIRA, R. de M. S.; SILVA, A. B.; SATO, T. P.; SÁ, J. C. de; SANTOS, A. C. G. dos; AMORIM FILHO, E. F.; VALE, T. L. do; GAZÊTA, G. S. |
|
Afiliação: |
Rita de Maria Seabra Nogueira, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Arannadia Barbosa Silva, Laboratório de Referência Nacional em Vetores das Riquetsioses - LIRN/IOC/Fiocruz; Tayra Pereira Sato, Laboratório de Referência Nacional em Vetores das Riquetsioses - LIRN/IOC/Fiocruz; Joicy Cortez de Sá, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Ana Clara Gomes dos Santos, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Edvaldo Franco Amorim Filho, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Tássia Lopes do Vale, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Gilberto Salles Gazêta, Laboratório de Referência Nacional em Vetores das Riquetsioses - LIRN/IOC/Fiocruz. |
|
Título: |
Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 12, p. 1416-1422, dezembro 2017. |
|
Idioma: |
Inglês |
|
Notas: |
Título em português: Detecção molecular e serológica de Theileria equi, Babesia caballi e Anaplasma phagocytophilum em equinos e carrapatos no Maranhão. |
|
Conteúdo: |
Equine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. equi 18S rRNA gene sequences in GenBank and 98 to 100% with B. caballi. Genetic analysis classified the obtained sequences as T. equi and B. caballi cluster, respectively. It can be concluded that these pathogens occur and are circulating in the studied area. MenosEquine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. ... Mostrar Tudo |
|
Palavras-Chave: |
Doença transmitida por vetores; Vector-borne disease. |
|
Thesagro: |
Carrapato; Cavalo. |
|
Thesaurus NAL: |
Anaplasma phagocytophilum; Babesia caballi; Brazil; Horses; Theileria equi; Ticks. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174112/1/Molecular-and-serological-detection-of-Theileria.pdf
|
|
Marc: |
LEADER 02954naa a2200337 a 4500
001 2089348
005 2018-03-19
008 2017 bl uuuu u00u1 u #d
100 1 $aNOQUEIRA, R. de M. S.
245 $aMolecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Detecção molecular e serológica de Theileria equi, Babesia caballi e Anaplasma phagocytophilum em equinos e carrapatos no Maranhão.
520 $aEquine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. equi 18S rRNA gene sequences in GenBank and 98 to 100% with B. caballi. Genetic analysis classified the obtained sequences as T. equi and B. caballi cluster, respectively. It can be concluded that these pathogens occur and are circulating in the studied area.
650 $aAnaplasma phagocytophilum
650 $aBabesia caballi
650 $aBrazil
650 $aHorses
650 $aTheileria equi
650 $aTicks
650 $aCarrapato
650 $aCavalo
653 $aDoença transmitida por vetores
653 $aVector-borne disease
700 1 $aSILVA, A. B.
700 1 $aSATO, T. P.
700 1 $aSÁ, J. C. de
700 1 $aSANTOS, A. C. G. dos
700 1 $aAMORIM FILHO, E. F.
700 1 $aVALE, T. L. do
700 1 $aGAZÊTA, G. S.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 12, p. 1416-1422, dezembro 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
